Epigallocatechin gallate (EGCG) suppresses lipopolysaccharide‐induced inflammatory bone resorption, and protects against alveolar bone loss in mice

Abstract

HighlightsWe examine the role of EGCG, a major polyphenol in green tea, in bone metabolism.LPS is a pathogen-associated molecule, and induces inflammatory bone resorption.EGCG suppresses the LPS-induced PGE production in osteoblasts.EGCG suppresses the LPS-induced bone resorption of alveolar bones in vitro.In the mouse model of periodontitis, EGCG restores the loss of alveolar bone mass.

Effects of O‐methylated (−)‐epigallocatechin gallate (EGCG) on LPS‐induced osteoclastogenesis, bone resorption, and alveolar bone loss in mice

“…1). In our previous study, the treatment of 30-90 lM EGCG dose dependently suppressed LPSinduced bone resorption [15], but 3-10 lM EGCG3″ Me clearly suppressed the bone resorption in the present study. Therefore, the inhibitory potency of EGCG3″Me seems to be slightly stronger than that of EGCG in bone resorption.…”

“…Using a mouse experimental model of periodontitis, we previously showed the potential effects of the green tea catechin EGCG in preventing alveolar bone loss . Here, we showed that EGCG3″Me also prevents alveolar bone loss in mice.…”

“…In a mouse model of periodontitis, LPS‐induced bone resorption was suppressed by EGCG3″Me in organ culture of alveolar bone, and the alveolar bone loss was attenuated by the injection of EGCG3″Me into the lower gingiva in vivo . The potency of EGCG3″Me in this model is slightly stronger than that of EGCG . The mode of retention of EGCG3″Me in the gingival tissues must be examined in order to compare the effects of EGCG3″Me and EGCG in this model.…”

“…We previously reported that EGCG suppresses LPS‐induced bone resorption via the negative regulation of PGE 2 production by osteoblasts and that EGCG restored alveolar bone loss induced by LPS in a mouse model of periodontitis . However, the effects of EGCG3″Me on bone resorption are not known.…”

A functional chitosan membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regeneration in dogs

“…A cross-sectional study reported that consumption of ≥1 cup/day of green tea decreased the odds ratio for tooth loss (Koyama et al, 2010). EGCG inhibits lipopolysaccharide-induced osteoclastic bone resorption in vitro and attenuates inflammatory bone loss of the mouse mandibular alveolar bone in vivo (Tominari et al, 2015). Local delivery of green tea catechin has been used as an effective adjunctive therapy along with scaling and root planing for the treatment of 8 chronic periodontitis (Gadagi, Chava & Reddy, 2013;Hattarki, Pushpa & Bhat, 2013;Hirasawa, Takada, Makimura & Otake, 2002).…”