Epigallocatechin gallate induced apoptosis in Sarcoma180 cells in vivo: Mediated by p53 pathway and inhibition in U1B, U4-U6 UsnRNAs expression

Manna, Sukumar; Banerjee, Soumya; Mukherjee, Sudeshna; Das, Sukta; Panda, Chinmay K.

doi:10.1007/s10495-006-0198-2

Cited by 37 publications

(22 citation statements)

References 58 publications

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“…Promotion of apoptotic cell death by EGCG in sarcoma 180 cells was induced through G2/M cell cycle arrest, down-regulation of Bcl-2 and c-myc, up-regulation of p53 and Bax and without changes in the expression of p21, p27, Bcl-x L , mdm2 and cyclin D1 [96]. Up-regulation of p21, p53 and Bax have also been reported in prostate cancer PC-3 cells together with increased levels of caspases-3, -9 and proteolytic cleavage of poly ADP-ribose polymerase (PPARc) [68].…”

Section: Apoptosismentioning

confidence: 94%

Cancer chemoprevention and chemotherapy: Dietary polyphenols and signalling pathways

Ramos¹

2008

Molecular Nutrition Food Res

628

499

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Prevention of cancer through dietary intervention recently has received an increasing interest, and dietary polyphenols have become not only important potential chemopreventive, but also therapeutic, natural agents. Polyphenols have been reported to interfere at the initiation, promotion and progression of cancer. They might lead to the modulation of proteins in diverse pathways and require the integration of different signals for the final chemopreventive or therapeutic effect. Polyphenols have been demonstrated to act on multiple key elements in signal transduction pathways related to cellular proliferation, differentiation, apoptosis, inflammation, angiogenesis and metastasis; however, these molecular mechanisms of action are not completely characterized and many features remain to be elucidated. The aim of this review is to provide insights into the molecular basis of potential chemopreventive and therapeutic activities of dietary polyphenols with emphasis in their ability to control intracellular signalling cascades considered as relevant targets in a cancer preventive approach.

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Section: Apoptosismentioning

confidence: 94%

Cancer chemoprevention and chemotherapy: Dietary polyphenols and signalling pathways

Ramos¹

2008

Molecular Nutrition Food Res

628

499

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“…The slides were washed with PBS and finally incubated with horseradish POD conjugated anti-fluorescein antibody and DAB as the chromogen for 30 mins at 37°C under humidified conditions. Stained slides were visualized and photographed under a bright field microscope (Manna et al, 2006).…”

Section: 6419 Reduction Of Proliferation and Induction Of Apoptosis mentioning

confidence: 99%

Reduction of Proliferation and Induction of Apoptosis are Associated with Shrinkage of Head and Neck Squamous Cell Carcinoma due to Neoadjuvant Chemotherapy

Sarkar

Maiti²,

Jha³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Neoadjuvant chemotherapy (NACT) is a treatment modality whereby chemotherapy is used as the initial treatment of HNSCC in patients presenting with advanced cancer that cannot be treated by other means. It leads to shrinkage of tumours to an operable size without significant compromise to essential oro-facial organs of the patients. The molecular mechanisms behind shrinkage due to NACT is not well elucidated. Materials and Methods: Eleven pairs of primary HNSCCs and adjacent normal epithelium, before and after chemotherapy were screened for cell proliferation and apoptosis. This was followed by immunohistochemical analysis of some cell cycle (LIMD1, RBSP3, CDC25A, CCND1, cMYC, RB, pRB), DNA repair (MLH1, p53) and apoptosis (BAX, BCL2) associated proteins in the same set of samples. Results: Significant decrease in proliferation index and increase in apoptotic index was observed in post-therapy tumors compared to pre-therapy. Increase in the RB/ pRB ratio, along with higher expression of RBSP3 and LIMD1 and lower expression of cMYC were observed in post-therapy tumours, while CCND1 and CDC25A remained unchanged. While MLH1 remained unchanged, p53 showed higher expression in post-therapy tumors, indicating inhibition of cell proliferation and induction of apoptosis. Increase in the BAX/BCL2 ratio was observed in post-therapy tumours, indicating up-regulation of apoptosis in response to therapy. Conclusions: Thus, modulation of the G1/S cell cycle regulatory proteins and apoptosis associated proteins might play an important role in tumour shrinkage due to NACT.

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“…The anti-tumor effect of flavonoids could also be exerted through the stimulation of apoptotic death, via activation of caspases (caspase-3, -9, and -8) and pro-apoptotic proteins (p53, p27 and Bax) as well as by inhibiting antiapoptotic proteins (Bcl-2 and Bid) and the release of cytochrome c [155][156][157][158].…”

Section: Effects On Cancer Developmentmentioning

confidence: 99%

Design and Development of Nanovehicle-Based Delivery Systems for Preventive or Therapeutic Supplementation with Flavonoids

Leonarduzzi

Testa

Sottero³

et al. 2010

CMC

128

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To date more than 4000 compounds are recognized to belong to the class of flavonoids. These natural phenolic drugs are poorly soluble in water and are rapidly degraded and metabolized in the human body, but nevertheless are very promising for their potential contribution to the prevention and therapy of major chronic diseases, including cardiovascular and neurodegenerative diseases and cancer. In recent years a number of flavanols (e.g. catechins), flavonols (e.g. quercetin, myricetin) and isoflavones (e.g. genistein, daidzein) have been confirmed to possess strong antioxidant, anti-inflammatory, anti-proliferative and anti-aging activities. Incorporation into lipidic or polymer-based nanoparticles appears to markedly help the oral delivery of flavonoids, as these particles can protect the drug from degradation in the gastrointestinal tract and, by virtue of their unique absorption mechanism through the lymphatic system, also from first-pass metabolism in the liver. In addition, both oral and parenteral administration of flavonoids exploits a pharmacologic delivery route that guarantees sustained release of the active principle at the desired site of action. A comprehensive review of studies currently available on the in vitro and in vivo experimental administration of flavonoids by means of nanovectors may be of use as a foundation for the development of advanced delivery systems for these powerful compounds, in view of their adoption in primary and secondary disease prevention.

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Epigallocatechin gallate induced apoptosis in Sarcoma180 cells in vivo: Mediated by p53 pathway and inhibition in U1B, U4-U6 UsnRNAs expression

Cited by 37 publications

References 58 publications

Cancer chemoprevention and chemotherapy: Dietary polyphenols and signalling pathways

Cancer chemoprevention and chemotherapy: Dietary polyphenols and signalling pathways

Reduction of Proliferation and Induction of Apoptosis are Associated with Shrinkage of Head and Neck Squamous Cell Carcinoma due to Neoadjuvant Chemotherapy

Design and Development of Nanovehicle-Based Delivery Systems for Preventive or Therapeutic Supplementation with Flavonoids

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