(‐)‐Epigallocatechin gallate‐loaded polycaprolactone scaffolds fabricated using a 3D integrated moulding method alleviate immune stress and induce neurogenesis

Abstract

Objectives In peripheral neuropathy, the underlying mechanisms of nerve and muscle degeneration include chronic inflammation and oxidative stress in fibrotic tissues. (‐)‐Epigallocatechin gallate (EGCG) is a major, active component in green tea and may scavenge free radical oxygen and attenuate inflammation. Conservative treatments such as steroid injection only deal with early, asymptomatic, peripheral neuropathy. In contrast, neurolysis and nerve conduit implantation work effectively for treating advanced stages. Materials and methods An EGCG‐loaded polycaprolactone (PCL) porous scaffold was fabricated using an integrated moulding method. We evaluated proliferative, oxidative and inflammatory activity of rat Schwann cells (RSCs) and rat skeletal muscle cells (RSMCs) cultured on different scaffolds in vitro. In a rat radiation injury model, we assessed the morphological, electrophysiological and functional performance of regenerated sciatic nerves and gastrocnemius muscles, as well as oxidative stress and inflammation state. Results RSCs and RSMCs exhibited higher proliferative, anti‐oxidant and anti‐inflammatory states in an EGCG/PCL scaffold. In vivo studies showed improved nerve and muscle recovery in the EGCG/PCL group, with increased nerve myelination and muscle fibre proliferation and reduced macrophage infiltration, lipid peroxidation, inflammation and oxidative stress indicators. Conclusions The EGCG‐modified PCL porous nerve scaffold alleviates cellular oxidative stress and repairs peripheral nerve and muscle structure in rats. It attenuates oxidative stress and inflammation in vivo and may provide further insights into peripheral nerve repair in the future.

Epigallocatechin-3-Gallate (EGCG), an Active Compound of Green Tea Attenuates Acute Lung Injury Regulating Macrophage Polarization and Krüpple-Like-Factor 4 (KLF4) Expression

“…We found enhanced expression of Ang-1 after treatment with EGCG in LPS-induced ALI. Similar to this, other studies showed beneficial effects on the regenerative capacity of different lung cells [ 48 , 49 ]. The effect of EGCG might be mediated through KLF4, which is a well−known regulator of stem cells and has a role in cell proliferation [ 12 , 50 ].…”

Preclinical assessment on neuronal regeneration in the injury-related microenvironment of graphene-based scaffolds

“…It is biocompatible and its biodegradation products are also toxic-free in the living body. Moreover, PCL provides certain mechanical stability with its high stiffness and relatively low degradation rate 32 , 33 . Consequently, the composite scaffold guarantees long-term in vivo support of structural integrity.…”

Rectify the Injury-Induced Microenvironment Imbalance in Peripheral Nerve Repair

“…In addition, we analysed the ability of our green teaderived (-) -Epigallocatechin gallate-loaded NGC to scavenge free radical oxygen by examining similar biomarkers, and demonstrated the importance of rectifying the energy metabolic disturbance again [11].…”