Epigenetic changes detected in micropropagated hop plants

Peredo, Elena L.; Arroyo-García, Rosa; Revilla, M. A.

doi:10.1016/j.jplph.2008.12.015

Cited by 48 publications

(48 citation statements)

References 35 publications

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“…Molecular analysis is being commonly used for monitoring genetic fidelity of in vitro raised plants. DNA based markers provide an effective procedure to determine tissue culture induced variations since these markers are not influenced by environmental factors (Peredo et al, 2009). PCR-based techniques such as SSR, ISSR, RAPD and AFLP have been used for as-…”

Section: Assessment Of Genetic Stability Using Issr Analysismentioning

confidence: 99%

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Samarfard¹,

Kadir²,

Kadzimin³

et al. 2013

Not Bot Hort Agrobot Cluj

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Chitosan is a carbohydrate polymer derivative of chitin which presents in shell of crustaceans. This biopolymer is a non toxic and environmentally friendly, considered as a plant growth stimulator in some plant species. The present study investigates the effects of chitosan and media types on multiplication and genetic stability of Phalaenopsis gigantea protocorm-like bodies (PLBs). PLBs were inoculated in liquid New Dogashima Medium (NDM) and Vacin and Went (VW) supplemented with various concentrations of chitosan (0, 5, 10, 15, 20 and 25 mg/L). The highest PLB multiplication was observed on VW and NDM supplemented with 10 mg/L chitosan with mean number of PLBs 177 and 147, respectively. Chitosan promoted the formation of juvenile leaves and the highest number was observed in NDM supplemented with 20 mg/L chitosan with mean number of 66 leaves after 8 weeks of culture. Genetic stability was assessed among mother plant and secondary PLBs after 2, 4, 6, and 8 weeks of culture in liquid media. 8 out of 10 ISSR markers produced a total of 275 clear and reproducible bands with mean of 6.9 bands per primer. The secondary PLBs produced during sub-culturing process of chitosan treated liquid culture were genetically uniform and similar to mother plant.

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Section: Assessment Of Genetic Stability Using Issr Analysismentioning

confidence: 99%

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Samarfard¹,

Kadir²,

Kadzimin³

et al. 2013

Not Bot Hort Agrobot Cluj

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show abstract

“…It is generally believed that in this way very little or no genetic variation is induced (De Klerk 1990). Peredo et al (2009) evaluated hop cultures with molecular techniques namely random amplified DNA polymorphism (RAPD), retrotransposon microsatellite amplified polymorphism (REMAP), and methylation-sensitive amplification polymorphism (MSAP). They did not detect any genetic variation between the original plants and propagated plants even after 12 cycles (2 years) of micropropagation.…”

Section: Detection Of Epigenetic Statusmentioning

confidence: 99%

Epigenetics in plant tissue culture

Smulders¹,

Klerk²

2010

Plant Growth Regul

220

136

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Plants produced vegetatively in tissue culture may differ from the plants from which they have been derived. Two major classes of off-types occur: genetic ones and epigenetic ones. This review is about epigenetic aberrations. We discuss recent studies that have uncovered epigenetic modifications at the molecular level, viz., changes in DNA methylation and alterations of histone methylation or acetylation. Various studies have been carried out with animals, and with plant cells or tissues that have grown in tissue culture but only little work has been done with shoots generated by axillary branching. We present various molecular methods that are being used to measure epigenetic variation. In micropropagated plants mostly differences in DNA methylation have been examined. Epigenetic changes are thought to underlie various well-known tissue-culture phenomena including rejuvenation, habituation, and morphological changes such as flower abnormalities, bushiness, and tumorous outgrowths in, among others, oil palm, gerbera, Zantedeschia and rhododendron.

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“…To study the extent of changes induced by tissue culture and inherited by the progeny different methods have been used (Hossain et al 2003;Peredo et al 2009;Linacero et al 2011;Díaz-Martínez et al 2012). Most of them involved isoschizomers differing in their sensitivity towards DNA methylation sites (Ochogavía et al 2009;Wang et al 2013;Machczyńska et al 2014).…”

Section: Introductionmentioning

confidence: 99%

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

Machczyńska

Orłowska

Mańkowski

et al. 2014

Plant Cell Tiss Organ Cult

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Doubled haploids of triticale are of interest for plant breeders due to hybrid breeding programs based on cytoplasmic male sterility Tt phenomenon. However, (epi)mutations appearing during in vitro culture regeneration may lead to a phenotypic variation that makes the uniformity of plant materials questionable. Using RP-HPLC genomic DNA methylation of donor doubled haploid plants utilized as a source of tissues for the in vitro regeneration (via androgenesis and somatic embryogenesis) of triticale cv. Bogo and their consecutive generative progeny was evaluated. It was demonstrated that in vitro cultures induced a decrease of the DNA methylation of the regenerants independently of the approach used for plant regeneration. The decrease in DNA methylation of genomic DNA proceeded up to the first/second successive generations followed by the beginning of its reestablishment. Moreover, somatic embryogenesis resulted in a higher level of genomic DNA demethylation in regenerants than androgenesis and the process of methylation seems to be affected by donor plant. It is being speculated that long term changes in genomic DNA methylation may be a source of off-type individuals that may spontaneously arise during plant breeding.

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Epigenetic changes detected in micropropagated hop plants

Cited by 48 publications

References 35 publications

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Epigenetics in plant tissue culture

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

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