Epigenetic Characterization of the CpG Islands of Bovine Leptin and POU5F1 Genes in Cloned Bovine Fetuses

Kremenskoy, Maksym; Kremenska, Yuliya; Suzuki, Masako; Imai, Kyoichi; Takahashi, Seiya; Hashizume, Kazuyoshi; Yagi, Shusuke; Shiota, Kunio

doi:10.1262/jrd.17100

Cited by 21 publications

(15 citation statements)

References 34 publications

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“…Humans have eight CpG islands in the Oct4 DMR while chimps have only six. For the bovine Oct4 gene, we validated the DMR in Intron 1, which was previously described by others (Kremenskoy et al, 2006). For bovine NANOG, we determined the proximal promoter region of the gene and designed bisulfite primers (Table 4) with the help of Methprimer software (http://www.urogene.org/methprimer).…”

Section: Oct4 and Nanog Demethylation In Iscnt And Scnt Embryosmentioning

confidence: 99%

Bovine Ooplasm Partially Remodels Primate Somatic Nuclei following Somatic Cell Nuclear Transfer

Wang

Beyhan

Rodríguez

et al. 2009

Cloning and Stem Cells

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Interspecies somatic cell nuclear transfer (iSCNT) has the potential to become a useful tool to address basic questions about the nucleus-cytoplasm interactions between species. It has also been proposed as an alternative for the preservation of endangered species and to derive autologous embryonic stem cells. Using chimpanzee/ bovine iSCNT as our experimental model we studied the early epigenetic events that take place soon after cell fusion until embryonic genome activation (EGA). Our analysis suggested partial EGA in iSCNT embryos at the eight-cell stage, as indicated by Br-UTP incorporation and expression of chimpanzee embryonic genes. Oct4, Stella, Crabp1, CCNE2, CXCL6, PTGER4, H2AFZ, c-MYC, KLF4, and GAPDH transcripts were expressed, while Nanog, Glut1, DSC2, USF2, Adrbk1, and Lin28 failed to be activated. Although development of iSCNT embryos did not progress beyond the 8- to 16-cell stage, chromatin remodeling events, monitored by H3K27 methylation, H4K5 acetylation, and global DNA methylation, were similar in both intra- and interspecies SCNT embryos. However, bisulfite sequencing indicated incomplete demethylation of Oct4 and Nanog promoters in eight-cell iSCNT embryos. ATP production levels were significantly higher in bovine SCNT embryos than in iSCNT embryos, TUNEL assays did not reveal any difference in the apoptotic status of the nuclei from both types of embryos. Collectively, our results suggest that bovine ooplasm can partially remodel chimpanzee somatic nuclei, and provides insight into some of the current barriers iSCNT must overcome if further embryonic development is to be expected.

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Section: Oct4 and Nanog Demethylation In Iscnt And Scnt Embryosmentioning

confidence: 99%

Bovine Ooplasm Partially Remodels Primate Somatic Nuclei following Somatic Cell Nuclear Transfer

Wang

Beyhan

Rodríguez

et al. 2009

Cloning and Stem Cells

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“…However, in the bovine embryo, there is again only limited evidence regarding epigenetic modification of trophoblastspecific genes (Kremenskoy et al 2006a(Kremenskoy et al , 2006b. Cattle generated by somatic cell nuclear transfer demonstrate various abnormalities, including placental malformation, which are thought to be caused by aberrant epigenetic regulation , Hall et al 2005, Arnold et al 2006b, Rybouchkin et al 2006, and suggests that trophoblast cell differentiation is under the control of epigenetic regulation as well.…”

Section: Roles Of Trophoblast-specific Genes From Gastrulation To Permentioning

confidence: 99%

Blastocyst elongation, trophoblastic differentiation, and embryonic pattern formation

Blomberg¹,

Hashizume²,

Viebahn³

2008

Reproduction

Self Cite

107

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The molecular basis of ungulate and non-rodent conceptus elongation and gastrulation remains poorly understood; however, use of stateof-the-art genomic technologies is beginning to elucidate the mechanisms regulating these complicated processes. For instance, transcriptome analysis of elongating porcine concepti indicates that protein synthesis and trafficking, cell growth and proliferation, and cellular morphology are major regulated processes. Furthermore, potential autocrine roles of estrogen and interleukin-1-b in regulating porcine conceptus growth and remodeling and metabolism have become evident. The importance of estrogen in pig is emphasized by the altered expression of essential steroidogenic and trophoblast factors in lagging ovoid concepti. In ruminants, the characteristic mononucleate trophoblast cells differentiate into a second lineage important for implantation, the binucleate trophoblast, and transcriptome profiling of bovine concepti has revealed a gene cluster associated with rapid trophoblast proliferation and differentiation. Gene cluster analysis has also provided evidence of correlated spatiotemporal expression and emphasized the significance of the bovine trophoblast cell lineage and the regulatory mechanism of trophoblast function. As a part of the gastrulation process in the mammalian conceptus, specification of the germ layers and hence definitive body axes occur in advance of primitive streak formation. Processing of the transforming growth factor-b-signaling molecules nodal and BMP4 by specific proteases is emerging as a decisive step in the initial patterning of the pre-gastrulation embryo. The topography of expression of these and other secreted molecules with reference to embryonic and extraembryonic tissues determines their local interaction potential. Their ensuing signaling leads to the specification of axial epiblast and hypoblast compartments through cellular migration and differentiation and, in particular, the specification of the early germ layer tissues in the epiblast via gene expression characteristic of endoderm and mesoderm precursor cells.

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“…Ce niveau de méthylation spermatique joue un rôle important dans l'obtention d'embryons évolutifs en AMP. Voilà qui n'est pas sans évoquer les problèmes rencontrés lors du clonage des animaux, où un défaut de méthyla-tion est souvent incriminé dans les échecs de clonage [17]. Reste que le mode de reprogrammation du matériel génétique des pronucléi n'est pas connu, non plus que les mécanismes inhérents à la mise en place de l'empreinte parentale.…”

Section: Resultsunclassified

Méthylation du spermatozoïde en Assistance médicale à la procréation (AMP)

Benchaïb

Ajina

Braun

et al. 2006

Gynécologie Obstétrique & Fertilité

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Epigenetic Characterization of the CpG Islands of Bovine Leptin and POU5F1 Genes in Cloned Bovine Fetuses

Cited by 21 publications

References 34 publications

Bovine Ooplasm Partially Remodels Primate Somatic Nuclei following Somatic Cell Nuclear Transfer

Bovine Ooplasm Partially Remodels Primate Somatic Nuclei following Somatic Cell Nuclear Transfer

Blastocyst elongation, trophoblastic differentiation, and embryonic pattern formation

Méthylation du spermatozoïde en Assistance médicale à la procréation (AMP)

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