Epigenetic regulation of Wnt7b expression by the cis-acting long noncoding RNA Lnc-Rewind in muscle stem cells

Cipriano, Andrea; Macino, Martina; Buonaiuto, Giulia; Santini, Tiziana; Biferali, Beatrice; Peruzzi, Giovanna; Colantoni, Alessio; Mozzetta, Chiara; Ballarino, Monica

doi:10.7554/elife.54782

Cited by 26 publications

(13 citation statements)

References 64 publications

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“…This result was also mirrored in vivo in cardiotoxin-induced mice in addition to a reduced number of newly formed myofibers [170]. A further example is Lnc-Rewind (Repressor of wnt induction), a chromatin-associated lncRNA previously identified by transcriptomic analysis [187] and recently shown to act as an epigenetic regulator of satellite cells proliferation and expansion [172]. Mechanistically, Lnc-Rewind directly interacts with the methyltransferase G9a to mediate the repression of its neighboring gene, Wnt7b, the expression of which is important for satellite cells' differentiation (Figure 3C).…”

Section: Nuclear Ncrnasmentioning

confidence: 78%

“…Mechanistically, Lnc-Rewind directly interacts with the methyltransferase G9a to mediate the repression of its neighboring gene, Wnt7b, the expression of which is important for satellite cells' differentiation (Figure 3C). Consistently, the depletion of Lnc-Rewind in MuSC-derived myoblasts leads to Wnt7b de-repression and to the aberrant expansion and activation of satellite pools on single myofibers [172]. Finally, we cannot fail to mention H19, one of the earliest lncRNAs to be identified and shown to exert diverse functions in varying types of cells, including satellite, cardiac, and skeletal muscle cells [188,189].…”

Section: Nuclear Ncrnasmentioning

confidence: 88%

“…In the nucleus, lncRNAs can influence gene expression at the epigenetic level through several mechanisms [65]. Examples in the figure include (B) lncRNA decoys: LncMAAT impedes SOX6 binding on the promoter of miR-29b to repress its transcription [169], Linc-YY1 binds YY1 and blocks its interaction with the PRC2 complex [170]; (C) lncRNA guides: CPR [171] and Lnc-Rewind [172] respectively interact with the DNMT3A and G9a repressive complexes and guide them on specific promoters. Dashed grey lines represent the loss of interaction and regulation.…”

Section: Cytoplasmic Ncrnasmentioning

confidence: 99%

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Muscle Regeneration and RNA: New Perspectives for Ancient Molecules

Buonaiuto

Desideri

Taliani

et al. 2021

Cells

Self Cite

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The ability of the ribonucleic acid (RNA) to self-replicate, combined with a unique cocktail of chemical properties, suggested the existence of an RNA world at the origin of life. Nowadays, this hypothesis is supported by innovative high-throughput and biochemical approaches, which definitively revealed the essential contribution of RNA-mediated mechanisms to the regulation of fundamental processes of life. With the recent development of SARS-CoV-2 mRNA-based vaccines, the potential of RNA as a therapeutic tool has received public attention. Due to its intrinsic single-stranded nature and the ease with which it is synthesized in vitro, RNA indeed represents the most suitable tool for the development of drugs encompassing every type of human pathology. The maximum effectiveness and biochemical versatility is achieved in the guise of non-coding RNAs (ncRNAs), which are emerging as multifaceted regulators of tissue specification and homeostasis. Here, we report examples of coding and ncRNAs involved in muscle regeneration and discuss their potential as therapeutic tools. Small ncRNAs, such as miRNA and siRNA, have been successfully applied in the treatment of several diseases. The use of longer molecules, such as lncRNA and circRNA, is less advanced. However, based on the peculiar properties discussed below, they represent an innovative pool of RNA biomarkers and possible targets of clinical value.

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Section: Nuclear Ncrnasmentioning

confidence: 78%

Section: Nuclear Ncrnasmentioning

confidence: 88%

Section: Cytoplasmic Ncrnasmentioning

confidence: 99%

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Muscle Regeneration and RNA: New Perspectives for Ancient Molecules

Buonaiuto

Desideri

Taliani

et al. 2021

Cells

Self Cite

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“…It interacts with G9a histone lysine methyl transferase to help recruit repressive histone marker H3K9me2 and repress the expression of Wnt7b. LncRewind depletion leads to MuSC differentiation defects (Cipriano et al 2021 ).…”

Section: Transcription and Epigenetic Regulation Of Musc Fate Transitionmentioning

confidence: 99%

Regulation of muscle stem cell fate

Zhuang

2022

Cell Regen

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Skeletal muscle plays a critical role in human health. Muscle stem cells (MuSCs) serve as the major cell type contributing to muscle regeneration by directly differentiating to mature muscle cells. MuSCs usually remain quiescent with occasionally self-renewal and are activated to enter cell cycle for proliferation followed by differentiation upon muscle injury or under pathological conditions. The quiescence maintenance, activation, proliferation, and differentiation of MuSCs are tightly regulated. The MuSC cell-intrinsic regulatory network and the microenvironments work coordinately to orchestrate the fate transition of MuSCs. The heterogeneity of MuSCs further complicates the regulation of MuSCs. This review briefly summarizes the current progress on the heterogeneity of MuSCs and the microenvironments, epigenetic, and transcription regulations of MuSCs.

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“…Numerous studies have indicated that lncRNAs exert either positive or negative effects to regulate the expression of neighboring protein-coding genes [20,21]. Therefore, we extracted the information related to the location of differential lncRNAs from the transcription file and porcine genome database (http://genome-asia.ucsc.edu) and selected mRNAs located within 10 kb upstream or downstream of these lncRNAs.…”

Section: Potential Cis-regulatory Mechanisms Between Porcine Delncrna...mentioning

confidence: 99%

Long non-coding RNAs in Sus scrofa ileum under starvation stress

Wang¹,

Ma²,

Li³

et al. 2022

Anim Biosci

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Objective: In this study, we aimed to identify long non-coding RNAs (lncRNAs) that play important roles in starvation stress, analyze their functions, and discover potential molecular targets to alleviate starvation stress, to provide a theoretical reference for subsequent in-depth research. Methods:We generated a piglet hunger stress animal model. Nine Yorkshire weaned piglets were randomly divided into a long-term hunger stress group (starved for 72 h), short-term hunger stress group (starved for 48 h), and the control group. LncRNA libraries were constructed using high-throughput sequencing of piglet ileums. Results:We obtained 11,792 lncRNAs, among which, 2500 lncRNAs were novel. In total, 509 differentially expressed (DE)lncRNAs were identified in this study. Target genes of DElncRNAs were predicted via cis and trans interactions, and functional and pathway analyses were performed. Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that lncRNA-targeted genes mainly participated in metabolic pathways, cellular processes, immune system processes, digestive systems, and transport activities. To reveal the mechanism underlying starvation stress, the interaction network between lncRNAs and their targets was constructed based on 26 DElncRNAs and 72 DE mRNAs. We performed an interaction network analysis of 121 DELncRNA-DEmRNA pairs with a Pearson correlation coefficient greater than 0.99.

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Epigenetic regulation of Wnt7b expression by the cis-acting long noncoding RNA Lnc-Rewind in muscle stem cells

Cited by 26 publications

References 64 publications

Muscle Regeneration and RNA: New Perspectives for Ancient Molecules

Muscle Regeneration and RNA: New Perspectives for Ancient Molecules

Regulation of muscle stem cell fate

Long non-coding RNAs in Sus scrofa ileum under starvation stress

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