Epigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates

Sparman, Michelle; Dighe, Vikas; Sritanaudomchai, Hathaitip; Ma, Hong; Ramsey, Cathy; Pedersen, Darlene; Clepper, Lisa; Nighot, Prashant K.; Wolf, Don P.; Hennebold, Jon D.; Mitalipov, Shoukhrat

doi:10.1002/stem.60

Cited by 41 publications

(45 citation statements)

References 42 publications

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“…Furthermore, we can add rabbit ES cells derived from somatic cell nuclear transfer (SCNT) for comparison because it is relatively easy to obtain blastocysts by SCNT in rabbits (21,27). In primates including humans, the generation of SCNT blastocysts is very difficult for unknown reasons, and only two SCNT-ES cell lines have been established (51). However, rabbit SCNT-ES cells are free of transgenes, and once reprogrammed to the totipotent state, they should be considered as cell resource candidates for future regenerative medicine.…”

Section: Discussionmentioning

confidence: 99%

Generation of Induced Pluripotent Stem Cells in Rabbits

Honda

Hirose

Hatori

et al. 2010

Journal of Biological Chemistry

157

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Human induced pluripotent stem (iPS) cells have the potential to establish a new field of promising regenerative medicine. Therefore, the safety and the efficiency of iPS-derived cells must be tested rigorously using appropriate animal models before human trials can commence. Here, we report the establishment of rabbit iPS cells as the first human-type iPS cells generated from a small laboratory animal species. Using lentiviral vectors, four human reprogramming genes (c-MYC, KLF4, SOX2, and OCT3/4) were introduced successfully into adult rabbit liver and stomach cells. The resulting rabbit iPS cells closely resembled human iPS cells; they formed flattened colonies with sharp edges and proliferated indefinitely in the presence of basic FGF. They expressed the endogenous pluripotency markers c-MYC, KLF4, SOX2, OCT3/4, and NANOG, whereas the introduced human genes were completely silenced. Using in vitro differentiating conditions, rabbit iPS cells readily differentiated into ectoderm, mesoderm, and endoderm. They also formed teratomas containing a variety of tissues of all three germ layers in immunodeficient mice. Thus, the rabbit iPS cells fulfilled all of the requirements for the acquisition of the fully reprogrammed state, showing high similarity to their embryonic stem cell counterparts we generated recently. However, their global gene expression analysis revealed a slight but rigid difference between these two types of rabbit pluripotent stem cells. The rabbit model should enable us to compare iPS cells and embryonic stem cells under the same standardized conditions in evaluating their ultimate feasibility for pluripotent cell-based regenerative medicine in humans.

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Section: Discussionmentioning

confidence: 99%

Generation of Induced Pluripotent Stem Cells in Rabbits

Honda

Hirose

Hatori

et al. 2010

Journal of Biological Chemistry

157

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“…Several researchers have examined the differentiation of primate ES cells into germ cells (Clark et al 2004, Chen et al 2007, Sparman et al 2009). When we examined the expression of three germ cell markers, CD9, DPPA3, and DDX4, in EBs derived from AgMES cells by RT-PCR, the expression of CD9 and DPPA3 was detected.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey

Shimozawa¹,

Nakamura²,

Takahashi³

et al. 2010

Reproduction

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Several cell types from the African green monkey (Cercopithecus aethiops), such as red blood cells, primary culture cells from kidney, and the Vero cell line, are valuable sources for biomedical research and testing. Embryonic stem (ES) cells that are established from blastocysts have pluripotency to differentiate into these and other types of cells. We examined an in vitro culture system of zygotes produced by ICSI in African green monkeys and attempted to establish ES cells. Culturing with and without a mouse embryonic fibroblast (MEF) cell monolayer resulted in the development of ICSI-derived zygotes to the blastocyst stage, while culturing with a buffalo rat liver cell monolayer yielded no development (3/14, 21.4% and 6/31, 19.4% vs 0/23, 0% respectively; P!0.05). One of the nine blastocysts, which had been one of the zygotes co-cultured with MEF cells, formed flat colonies consisting of cells with large nuclei, similar to other primate ES cell lines. The African green monkey ES (AgMES) cells expressed pluripotency markers, formed teratomas consisting of three embryonic germ layer tissues, and had a normal chromosome number. Furthermore, expression of the germ cell markers CD9 and DPPA3 (STELLA) was detected in the embryoid bodies, suggesting that AgMES cells might have the potential ability to differentiate into germ cells. The results suggested that MEF cells greatly affected the quality of the inner cell mass of the blastocysts. In addition, AgMES cells would be a precious resource for biomedical research such as other primate ES cell lines.

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“…Could this be used to shift cell type identity? Based on the findings from the field of nuclear reprograming -either by nuclear transfer, cell fusion or overexpression of master regulatory transcription factors -this would seem possible (Sparman et al 2009). …”

Section: Overviewmentioning

confidence: 99%

Programming and reprogramming neural cell types using synthetic transcription factors

Matjusaitis¹

2016

IET/SynbiCITE Engineering Biology Conference

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Epigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates

Cited by 41 publications

References 42 publications

Generation of Induced Pluripotent Stem Cells in Rabbits

Generation of Induced Pluripotent Stem Cells in Rabbits

Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey

Programming and reprogramming neural cell types using synthetic transcription factors

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