Epigenetic Signaling of Cancer Stem Cells During Inflammation

Ren, Yuqing; Meng, Lingfang; Li, Lifeng; Beatson, Richard; Deng, Jinhai; Zhang, Tengfei; Liu, Junqi; Han, Xinwei

doi:10.3389/fcell.2021.772211

Cited by 16 publications

(8 citation statements)

References 125 publications

(142 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CSCs are the key drivers of cancer and play a role in relapse, resistance to anticancer therapies, and tumor recurrence [ 32 ]. CSCs derived from breast cancer cells with CD44+/CD24 low/− phenotype have the ability of heterogeneous differentiation, initiating diverse tumors and forming mammospheres [ 33 , 34 , 35 , 36 ]. The mammosphere formation assay has been used as a useful method for studying stem cell-like characteristics in breast cancer cell cultures [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Role of a Mixture of Polyphenol Compounds Released after Blueberry Fermentation in Chemoprevention of Mammary Carcinoma: In Vivo Involvement of miR-145

Mallet

Shahbazi

Alsadi

et al. 2023

IJMS

View full text Add to dashboard Cite

Epigenetic mechanisms such as microRNA (miRNA) deregulation seem to exert a central role in breast cancer initiation and progression. Therefore, targeting epigenetics deregulation may be an effective strategy for preventing and halting carcinogenesis. Studies have revealed the significant role of naturally occurring polyphenolic compounds derived from fermented blueberry fruits in cancer chemoprevention by modulation of cancer stem cell development through the epigenetic mechanism and regulation of cellular signaling pathways. In this study, we first investigated the phytochemical changes during the blueberry fermentation process. Fermentation favored the release of oligomers and bioactive compounds such as protocatechuic acid (PCA), gallic acid, and catechol. Next, we investigated the chemopreventive potentials of a polyphenolic mixture containing PCA, gallic acid, and catechin found in fermented blueberry juice in a breast cancer model by measuring miRNA expression and the signaling pathways involved in breast cancer stemness and invasion. To this end, 4T1 and MDA-MB-231 cell lines were treated with different doses of the polyphenolic mixture for 24 h. Additionally, female Balb/c mice were fed with this mixture for five weeks; two weeks before and three weeks after receiving 4T1 cells. Mammosphere formation was assayed in both cell lines and the single-cell suspension obtained from the tumor. Lung metastases were counted by isolating 6-thioguanine-resistant cells present in the lungs. In addition, we conducted RT-qPCR and Western blot analysis to validate the expression of targeted miRNAs and proteins, respectively. We found a significant reduction in mammosphere formation in both cell lines treated with the mixture and in tumoral primary cells isolated from mice treated with the polyphenolic compound. The number of colony-forming units of 4T1 cells in the lungs was significantly lower in the treatment group compared to the control group. miR-145 expression significantly increased in the tumor samples of mice treated with the polyphenolic mixture compared to the control group. Furthermore, a significant increase in FOXO1 levels was noted in both cell lines treated with the mixture. Overall, our results show that phenolic compounds found in fermented blueberry delay the formation of tumor-initiating cells in vitro and in vivo and reduce the spread of metastatic cells. The protective mechanisms seem to be related, at least partly, to the epigenetic modulation of mir-145 and its signaling pathways.

show abstract

Section: Discussionmentioning

confidence: 99%

Role of a Mixture of Polyphenol Compounds Released after Blueberry Fermentation in Chemoprevention of Mammary Carcinoma: In Vivo Involvement of miR-145

Mallet

Shahbazi

Alsadi

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Epigenetic changes occur simultaneously with oncogenic events in cancer progression. In GBM, epigenetic modification including DNA methylation, histone modification, and non-coding RNA regulation could modulate the inflammatory genes-oncogenes loop and associated immunosuppression (92)(93)(94)(95), providing a therapeutic strategy and comprehensively recognized pathogenesis of GBM by targeting the inflammatory TME (Table 1).…”

Section: Epigenetic Regulations Of the Inflammatory Microenvironment ...mentioning

confidence: 99%

Epigenetic Underpinnings of Inflammation: A Key to Unlock the Tumor Microenvironment in Glioblastoma

Chen

Cheng

2022

Front. Immunol.

View full text Add to dashboard Cite

Glioblastoma (GBM) is the most common malignant brain tumor in adults, and immunotherapies and genetic therapies for GBM have evolved dramatically over the past decade, but GBM therapy is still facing a dilemma due to the high recurrence rate. The inflammatory microenvironment is a general signature of tumors that accelerates epigenetic changes in GBM and helps tumors avoid immunological surveillance. GBM tumor cells and glioma-associated microglia/macrophages are the primary contributors to the inflammatory condition, meanwhile the modification of epigenetic events including DNA methylation, non-coding RNAs, and histone methylation and deacetylases involved in this pathological process of GBM, finally result in exacerbating the proliferation, invasion, and migration of GBM. On the other hand, histone deacetylase inhibitors, DNA methyltransferases inhibitors, and RNA interference could reverse the inflammatory landscapes and inhibit GBM growth and invasion. Here, we systematically review the inflammatory-associated epigenetic changes and regulations in the microenvironment of GBM, aiming to provide a comprehensive epigenetic profile underlying the recognition of inflammation in GBM.

show abstract

“…Studies suggested that epigenetic regulation exerts a pivotal role in many diseases such as cancers and CVDs ( Liu et al, 2021 ; Yang et al, 2021 ). Distinct from genetic alteration, epigenetic modification is reversible, and the inhibitor of epigenetic alteration provides a novel insight into searching pharmaceutical therapeutic targets ( Pons et al, 2009 ).…”

Section: Epigenetic Regulation Of Atherosclerosismentioning

confidence: 99%

Epigenetic Regulation in Pathology of Atherosclerosis: A Novel Perspective

et al. 2021

View full text Add to dashboard Cite

Atherosclerosis, characterized by atherosclerotic plaques, is a complex pathological process that involves different cell types and can be seen as a chronic inflammatory disease. In the advanced stage, the ruptured atherosclerotic plaque can induce deadly accidents including ischemic stroke and myocardial infarction. Epigenetics regulation, including DNA methylation, histone modification, and non-coding RNA modification. maintains cellular identity via affecting the cellular transcriptome. The epigenetic modification process, mediating by epigenetic enzymes, is dynamic under various stimuli, which can be reversely altered. Recently, numerous studies have evidenced the close relationship between atherosclerosis and epigenetic regulations in atherosclerosis, providing us with a novel perspective in researching mechanisms and finding novel therapeutic targets of this serious disease. Here, we critically review the recent discoveries between epigenetic regulation mechanisms in atherosclerosis.

show abstract

Epigenetic Signaling of Cancer Stem Cells During Inflammation

Cited by 16 publications

References 125 publications

Role of a Mixture of Polyphenol Compounds Released after Blueberry Fermentation in Chemoprevention of Mammary Carcinoma: In Vivo Involvement of miR-145

Role of a Mixture of Polyphenol Compounds Released after Blueberry Fermentation in Chemoprevention of Mammary Carcinoma: In Vivo Involvement of miR-145

Epigenetic Underpinnings of Inflammation: A Key to Unlock the Tumor Microenvironment in Glioblastoma

Epigenetic Regulation in Pathology of Atherosclerosis: A Novel Perspective

Contact Info

Product

Resources

About