Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma

Ming, Fei; Sun, Qianqiang

doi:10.3892/mmr.2017.6665

Cited by 15 publications

(14 citation statements)

References 33 publications

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“…We and others reported that methylation is a crucial mechanism that determines PTPRO expression (15)(16)(17)(18). We, therefore, investigated whether PTPRO was methylated in renal cancer cell lines.…”

Section: Methylation Of Ptpro Was Associated With Its Expression and mentioning

confidence: 96%

PTPRO predicts patient prognosis and correlates with immune infiltrates in human clear cell renal cell carcinoma

Gan¹,

Zhang²

2020

Transl Cancer Res TCR

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Background: The tumor-suppressive role of protein tyrosine phosphatase receptor type O (PTPRO) has been described in a variety of human cancers; however, the clinical significance of PTPRO in human clear cell renal cell carcinoma (ccRCC) remains unclear.Methods: PTPRO expression in renal cell carcinoma (RCC) was analyzed via the Oncomine database, Gene Expression Omnibus (GEO) datasets, and The Cancer Genome Atlas (TCGA) datasets. The Kaplan-Meier curves and Cox proportional hazards model were used to evaluate the relationship of PTPRO with overall survival in ccRCC. Gene ontology (GO) analysis and gene set enrichment analysis (GSEA) were performed to explore the signaling pathways in which PTPRO may be involved. The correlation between PTPRO and immune infiltrates in ccRCC was investigated via Tumor Immune Estimation Resource (TIMER) database. The association between PTPRO mRNA expression and its methylation in RCC was analyzed using the Cancer Cell Line Encyclopedia (CCLE) dataset, GEO dataset, and cBioPortal database.The impact of PTPRO methylation on overall survival was estimated by the MethSurv database.Results: We showed that the expression of PTPRO was significantly lower in human RCC. Moreover, the lower expression of PTPRO was associated with worse overall survival in ccRCC, particularly in the advanced stage patients. Multivariate Cox regression analysis revealed the expression of PTPRO as an independent prognostic predictor for overall survival of ccRCC. Of note, PTPRO was found to be associated with the activation of immune signaling and immune cell infiltration. Furthermore, methylation of PTPRO was prevalently observed in ccRCC, and methylation of PTPRO predicted the poor outcome of ccRCC.Conclusions: Our findings suggested that PTPRO at both RNA and DNA methylation levels had the potential as a prognostic biomarker for predicting prognosis, and PTPRO expression was closely associated with immune infiltration in ccRCC patients.

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Section: Methylation Of Ptpro Was Associated With Its Expression and mentioning

confidence: 96%

PTPRO predicts patient prognosis and correlates with immune infiltrates in human clear cell renal cell carcinoma

Gan¹,

Zhang²

2020

Transl Cancer Res TCR

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“…Similarly, expression of PTPRO truncated isoform (PTPROt) is increased in liver macrophages with the increasing degree of nonalcoholic steatohepatitis [21]. In contrast, PTPRO is down-regulated in injured podocytes and lung squamous cell carcinoma compared with healthy tissues [22,23]. PTPRO may exhibit different expression alterations in different organs or diseases.…”

Section: Discussionmentioning

confidence: 99%

PTPRO knockdown protects against inflammation in hemorrhage shock-induced acute lung injury involving the NF-κB signaling pathway

HUAN¹,

Tang

XU³

et al. 2022

Preprint

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Background Hemorrhage shock (HS) is characterized by decreased tissue oxygenation and organ damage due to severe blood loss. Acute lung injury (ALI) is a common complication of HS. Protein tyrosine phosphatase receptor type O (PTPRO) is abnormally up-regulated in the rat lungs after trauma/HS. Methods To elucidate the regulatory mechanism of PTPRO in lung inflammation following HS, we established a rat model of HS via withdrawing blood by a catheter inserted into the femoral artery followed by resuscitation. The rats were infected with lentiviral encoding PTPRO short hairpin RNA (shRNA) by intratracheal instillation for PTPRO knockdown. Results PTPRO was significantly up-regulated in rat lungs after HS. PTPRO knockdown enhanced epithelial integrity and reduced capillary leakage by up-regulating tight junction proteins zonula occludens-1 (ZO-1) and occludin (OCC). Besides, decreased myeloperoxidase expression and activity in the lungs indicated that HS-induced neutrophil infiltration into the lungs was mitigated by PTPRO knockdown. Meanwhile, expression of inflammatory cytokines/chemokines TNF-α, IL-6, MIP-2, MCP-2, and KC in the lungs or bronchoalveolar lavage fluid was regressed after PTPRO knockdown. The nuclear factor kappa B (NF-κB) pathway was related to inflammation in organ injury. PTPRO down-regulation had an inhibitory effect on the NF-κB pathway activation by suppressing the phosphorylation of NF-κB and its translocation from the cytoplasm into the nucleus in HS. Conclusion Taken together, we demonstrated that PTPRO knockdown may contribute to attenuating inflammation in HS-induced acute lung injury via inhibiting NF-κB pathway activation.

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“…Protein tyrosine phosphatase receptor-type O (PTPRO), a protein tyrosine phosphatase receptor, has been reported to be involved in the pathogenesis and progression of lung squamous cell carcinoma ( 4 ), breast cancer ( 5 ), colorectal cancer ( 6 ) and hepatocellular carcinoma ( 7 ). However, few studies have focused on the association between PTPRO and ACS.…”

Section: Introductionmentioning

confidence: 99%

Protein tyrosine phosphatase receptor‑type O expression as a prognostic marker in patients with acute coronary syndrome undergoing percutaneous coronary intervention: A prospective study

2021

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Optimal clinical indicators are crucial for evaluating the prognosis of patients with acute coronary syndrome (ACS). In the present study, the potential prognostic value of protein tyrosine phosphatase receptor-type O (PTPRO) expression in the peripheral blood mononuclear cells of patients with ACS undergoing percutaneous coronary intervention (PCI) was investigated. Patients diagnosed with ACS were prospectively recruited, and PTPRO expression in mononuclear cells separated from peripheral blood was assessed by western blotting. The prognosis was judged by the occurrence of major adverse cardiovascular events. Cox regression analyses were performed to assess the association between PTPRO expression and prognosis. In the enrolled 185 patients with ACS, PTPRO expression was lower after PCI compared with that before PCI (P<0.05). Although the pre-PCI PTPRO expression did not differ significantly between the good and poor prognosis groups, PTPRO expression after PCI was significantly lower in the good prognosis group compared with the poor prognosis group (P<0.05). The area under the receiver operating characteristic curve for the prognostic value of post-PCI PTPRO expression was significantly greater than that for pre-PCI PTPRO expression (P<0.05). Cox regression analysis identified high post-PCI PTPRO expression as an independent risk factor for poor prognosis in patients with ACS (P<0.05), and further analysis indicated that the post-PCI PTPRO expression level was associated with the prognosis of patients with ACS (P<0.05). PTPRO expression in peripheral blood mononuclear cells after PCI is associated with the prognosis of patients with ACS, with high PTPRO expression indicating a high risk of poor prognosis in patients with ACS.

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Epigenetically silenced PTPRO functions as a prognostic marker and tumor suppressor in human lung squamous cell carcinoma

Cited by 15 publications

References 33 publications

PTPRO predicts patient prognosis and correlates with immune infiltrates in human clear cell renal cell carcinoma

PTPRO predicts patient prognosis and correlates with immune infiltrates in human clear cell renal cell carcinoma

PTPRO knockdown protects against inflammation in hemorrhage shock-induced acute lung injury involving the NF-κB signaling pathway

Protein tyrosine phosphatase receptor‑type O expression as a prognostic marker in patients with acute coronary syndrome undergoing percutaneous coronary intervention: A prospective study

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