Epimedium koreanum Nakai Displays Broad Spectrum of Antiviral Activity in Vitro and in Vivo by Inducing Cellular Antiviral State

Cho, Won‐Kyung; Weeratunga, Prasanna; Lee, Byeong‐Hoon; Park, Jun-Seol; Kim, Chul-Joong; Yeul, Jin; Lee, Jong-Soo

doi:10.3390/v7010352

Cited by 58 publications

(30 citation statements)

References 63 publications

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“…Dried EF was purchased from Yeongcheon Oriental Herbal Market (Yeongcheon, South Korea) and stored in the KM-Application Center herbarium (registration number, #354) Korea Institute of Oriental Medicine(KIOM), after verification of identity by Prof. Ki Hwan Bae (Chungnam National University, Daejeon, Korea). The WEF was filtered through standard sieves (150 μm, Retsch, Haan, Germany), concentrated by lyophilization, and stored at −20°C until use (Cho et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

“…Madin-Darby Canine Kidney (MDCK, ATCC CCL-34, NBL-2), African green monkey kidney (Vero, ATCC CCL-81), and RAW264.7 (murine macrophage, ATCC TIB-71) cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; Lonza, Walkersville, MD) containing 10% fetal bovine serum (FBS; Cellgro, Manassas, VA, USA) and 1% antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin) at 37°C with 5% CO 2 as described previously (Cho et al, 2015 ). Influenza A/PuertoRico/8/34 H1N1, green fluorescent protein (GFP)-tagged Influenza A/PuertoRico/8/34 H1N1 (PR8-GFP), and NDV-GFP were propagated in the allantoic fluid of 10-day-old chicken embryos.…”

Section: Methodsmentioning

confidence: 99%

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Eupatorium fortunei and Its Components Increase Antiviral Immune Responses against RNA Viruses

et al. 2017

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Eupatorium fortunei (EF) has long been used as herbal medicine in Korea, China, and Asian countries to treat a variety of diseases. Recent studies have reported that EF has anti-metastatic, anti-angiogenic, anti-bacterial, and anti-oxidant activities, as well as activities against malignant metastatic human cancers. The effect of EF and its components on viruses has not been reported. In the present study, the antiviral activity and mechanism of action of an aqueous extract of EF (WEF) and its components were evaluated in vitro. We found that pretreatment with WEF markedly reduced viral replication, as evaluated using a green fluorescent protein (GFP)-tagged virus (influenza A virus, Newcastle disease virus, and vesicular stomatitis virus) in murine RAW 264.7 macrophage cells. We demonstrated that WEF induces the production of type I IFN including pro-inflammatory cytokines. Additionally, we identified the active anti-viral components of WEF as quercetin, psoralen, and quercitrin. Thus, WEF and its active components are immunomodulators of the innate immune response in murine macrophages, a finding that is potentially useful to developing prophylactic or therapeutic treatments against a range of viruses.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Eupatorium fortunei and Its Components Increase Antiviral Immune Responses against RNA Viruses

et al. 2017

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“…Quercetin also demonstrated a dose-dependent antiviral activity against poliovirus type 1, HSV-1, HSV-2, and respiratory syncytial virus (RSV) in cell cultures [60,83]. Epimedium koreanum Nakai, which contains quercetin as the major active component, has been shown to induce secretion of type I IFN, reducing the replication of HSV, Newcastle disease virus (NDV), vesicular stomatitis virus (VSV) in vitro, as well as influenza A subtypes (H1N1, H5N2, H7N3 and H9N2) in vivo [18]. Hung et al [51] have suggested possible mechanisms whereby quercetin may exert its anti-HSV activity.…”

Section: Antiviral Activity Of Flavonolsmentioning

confidence: 99%

Flavonoids: promising natural compounds against viral infections

et al. 2017

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Flavonoids are widely distributed as secondary metabolites produced by plants and play important roles in plant physiology, having a variety of potential biological benefits such as antioxidant, anti-inflammatory, anticancer, antibacterial, antifungal and antiviral activity. Different flavonoids have been investigated for their potential antiviral activities and several of them exhibited significant antiviral properties in in vitro and even in vivo studies. This review summarizes the evidence for antiviral activity of different flavonoids, highlighting, where investigated, the cellular and molecular mechanisms of action on viruses. We also present future perspectives on therapeutic applications of flavonoids against viral infections.

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“…Interestingly, one of the many biological effects of flavonoids is their antiviral activity. For example, apigenin extracted from Ocimum basilicum showed a potent antiviral activity against adenoviruses and hepatitis B virus (Chiang et al, 2005), whereas quercetin demonstrated a dose-dependent antiviral activity against herpes simplex virus (Lyu et al, 2005), Japanese encephalitis virus (Johari et al, 2012), hepatitis C virus in vitro (Rojas et al, 2016); and influenza A subtypes in vivo (Cho et al, 2015). Similarly, genistein was shown to inhibit HIV infection in resting CD4 T cells through interference with HIV-mediated actin dynamics (Guo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Genistein inhibits African swine fever virus replication in vitro by disrupting viral DNA synthesis

et al. 2018

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African swine fever virus (ASFV) is the causal agent of a highly-contagious and fatal disease of domestic pigs, leading to serious socio-economic consequences in affected countries. Once, neither an anti-viral drug nor an effective vaccines are available, studies on new anti-ASFV molecules are urgently need. Recently, it has been shown that ASFV type II topoisomerase (ASFV-topo II) is inhibited by several fluoroquinolones (bacterial DNA topoisomerase inhibitors), raising the idea that this viral enzyme can be a potential target for drug development against ASFV. Here, we report that genistein hampers ASFV infection at non-cytotoxic concentrations in Vero cells and porcine macrophages. Interestingly, the antiviral activity of this isoflavone, previously described as a topo II poison in eukaryotes, is maximal when it is added to cells at middle-phase of infection (8 hpi), disrupting viral DNA replication, blocking the transcription of late viral genes as well as the synthesis of late viral proteins, reducing viral progeny. Further, the single cell electrophoresis analysis revealed the presence of fragmented ASFV genomes in cells exposed to genistein, suggesting that this molecule also acts as an ASFV-topo II poison and not as a reversible inhibitor. No antiviral effects were detected when genistein was added before or at entry phase of ASFV infection. Molecular docking studies demonstrated that genistein may interact with four residues of the ATP-binding site of ASFV-topo II (Asn-144, Val-146, Gly-147 and Leu-148), showing more binding affinity (-4.62 kcal/mol) than ATP (-3.02 kcal/mol), emphasizing the idea that this viral enzyme has an essential role during viral genome replication and can be a good target for drug development against ASFV.

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Epimedium koreanum Nakai Displays Broad Spectrum of Antiviral Activity in Vitro and in Vivo by Inducing Cellular Antiviral State

Cited by 58 publications

References 63 publications

Eupatorium fortunei and Its Components Increase Antiviral Immune Responses against RNA Viruses

Eupatorium fortunei and Its Components Increase Antiviral Immune Responses against RNA Viruses

Flavonoids: promising natural compounds against viral infections

Genistein inhibits African swine fever virus replication in vitro by disrupting viral DNA synthesis

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