Epiphytic Survival of Erwinia tracheiphila on Muskmelon (Cucumis melo L.)

Abstract

Saalau Rojas, E., and Gleason, M. L. 2012. Epiphytic survival of Erwinia tracheiphila on muskmelon {Cucumis melo L.). Plant Dis. 96:62-66.72 h. In the second experiment, E, tracheiphila was monitored during alternating 12-h wet and dry periods, or eonfinuous wet or dry conditions for 48 h at 20°C. Survival of E. tracheiphila on wet muskmelon leaves depended on temperature {P < 0.01), with the greatest survival at 10 and 15°C and least at 30 and 35°C. Leaf wetness also impacted survival; an initial 12-h dry period resulted in a I.(XK)-to l().t)00-fold reduction in population si/e, followed by stabilization of the surviving population. These results demonstrate that E. tracheiphila can survive on muskmelon leaves under a wide range of environmental conditions, suggesting that epiphytic populations might serve as a reservoir of inoculum for infections.

Integration of Rhizobacterial Isolates and Airone Chemical for Effective Management of Bacterial Wilt in Cucumber (Cucumis sativus)

“…Significantly, the absence of translucency in the colonies hindered the transmission of light when subjected to an illumination source. These characteristics matched those documented in the literature for E. tracheiphila colonies (Rojas and Gleason, 2012;Mitchell and Hanks, 2009;De Mackiewicz et al, 1998). Biochemical tests revealed each isolate was test Gramnegative.…”

“…The meticulous selection of colonies resembling E. tracheiphila in morphology were transferred to new NA containing Petri plates incubated at the optimal temperature. The purified bacterial colonies were then preserved in a 50% glycerol solution at -20°C for future experimentation (Rojas and Gleason, 2012). The pathogenic bacteria were identified using a comprehensive approach that included the examination of physiological properties using microscopy techniques and comparison with previously documented bacterial strains in the literature (De Mackiewicz et al, 1998).…”

Surface Survival and Internalization of Salmonella through Natural Cracks on Developing Cantaloupe Fruits, Alone or in the Presence of the Melon Wilt Pathogen Erwinia tracheiphila

“…However, our preliminary experiments had revealed that E. tracheiphila could enter the fruit after introduction to the cracked areas (data not shown) or through flower interiors [60], and that watersoaked lesions often appeared within 4–5 days or 18 days after inoculation, respectively. Furthermore, Rojas and Gleason [61] recently reported that E. tracheiphila can live as an epiphyte on muskmelon leaves under a wide range of leaf wetness levels and temperatures, and they speculated that this niche could serve as a source of E. tracheiphila inoculum for pathogen dissemination. Their findings, combined with ours, suggest that E. tracheiphila may be a common resident on cucurbit plant surfaces in nature.…”