Epirubicin-Adsorbed Nanodiamonds Kill Chemoresistant Hepatic Cancer Stem Cells

Wang, Xin; Low, Xinyi Casuarine; Hou, Weixin; Abdullah, Lissa Nurrul; Toh, Tan Boon; Rashid, Masturah Bte Mohd Abdul; Ho, Dean; Chow, Edward K.

doi:10.1021/nn503491e

Cited by 180 publications

(179 citation statements)

References 83 publications

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“…On the contrary, after exposure to the ND + C solution, the amount of citropten detected in the whole cell compartment ( Figure 2C) and in each distinct cell ( Figure 2E) was less (0.02-0.1 nM) than that detected after the previous treatment with pure C. Indeed, the major fraction of citropten was always identifiable in the culture solution ( Figure 2C), probably because the release mechanism between ND and functionalized molecule was highly influenced by the pH value and protein concentration of the cell culture medium. 33,34 In particular, we observed that the most citropten was rapidly released from the NDs in the medium ( Figure 2D). On the other hand, we observed how the level of free C in each cell cytoplasm was maintained constant during the exposure time course while its ND-coupled form increased in concentration ( Figure 2F), supporting the idea that ND, through a not-yetknown mechanism, was able to inhibit/modulate the continuous uptake of free citropten into the cells.…”

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confidence: 83%

Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization

Gismondi¹,

Nanni²,

Reina³

et al. 2016

IJN

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For the first time, we coupled reduced detonation nanodiamonds (NDs) with a plant secondary metabolite, citropten (5,7-dimethoxycoumarin), and demonstrated how this complex was able to reduce B16F10 tumor cell growth more effectively than treatment with the pure molecule. These results encouraged us to find out the specific mechanism underlying this phenomenon. Internalization kinetics and quantification of citropten in cells after treatment with its pure or ND-conjugated form were measured, and it was revealed that the coupling between NDs and citropten was essential for the biological properties of the complex. We showed that the adduct was not able to induce apoptosis, senescence, or differentiation, but it determined cell cycle arrest, morphological changes, and alteration of mRNA levels of the cytoskeletal-related genes. The identification of metaphasic nuclei and irregular disposition of β-actin in the cell cytoplasm supported the hypothesis that citropten conjugated with NDs showed antimitotic properties in B16F10 cells. This work can be considered a pioneering piece of research that could promote and support the biomedical use of plant drug-functionalized NDs in cancer therapy

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confidence: 83%

Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization

Gismondi¹,

Nanni²,

Reina³

et al. 2016

IJN

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“…[17] Nakanishi et al have summarised the biological applications, especially cell growth, sensing, and control using nanoarchitectures of nanocarbons. [18] Wang et al [19] proposed that nanodiamondÀepirubicin drug delivery complex is capable of killing chemoresistant cancer stem cells and preventing secondary tumour formation in liver cancer. It has been noticed that compound naphthalocyanine encapsulated in water soluble polymer dendrimer can be used to kill the tumour and cancer cells in ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterisation and antimicrobial activity of manganese- and iron-doped zinc oxide nanoparticles

Sharma

Jandaik

Kumar

et al. 2015

Journal of Experimental Nanoscience

111

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The present work reports study on antimicrobial activity of pure and doped ZnO nanocomposites. Polyvinyl pyrrolidone capped Mn-and Fe-doped ZnO nanocomposites were synthesised using simple chemical co-precipitation technique. The synthesised materials were characterised using transmission electron microscope (TEM), X-ray powder diffraction (XRD), energy dispersive X-ray fluorescence (EDXRF), Fourier transform infrared (FTIR) spectroscopy and ultraviolet (UV) visible spectroscopy. The XRD and TEM studies reveal that the synthesised ZnO nanocrystals have a hexagonal wurtzite structure with average crystalline size »7À14 nm. EDXRF and FTIR study confirmed the doping and the incorporation of impurity in ZnO nanostructure. The antimicrobial activities of nanoparticles (NPs) were studied against fungi, grampositive and gram-negative bacteria using the standard disc diffusion method. The photocatalytic activities of prepared NPs were evaluated by degradation of methylene blue dye in aqueous solution under UV light irradiation. Experimental results demonstrated that ZnO NPs doped with 10% of Mn and Fe ions showed maximum antimicrobial and photodegradation efficiency in contrast with that of the 1% loading. The enhancement in antimicrobial effect and photocatalytic degradation is attributed to the generation of reactive oxygen species due to the synergistic effects of Mn and Fe loading.

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“…ND particles, which are capable of mediating improved efficacy and safety by forming complexes around the active agent while preserving its innate functionality (20)(21)(22)(23)(24)(25)(26)(27). ND safety and biocompatibility have been clearly demonstrated in numerous applications ranging from oncology to wound healing (28)(29)(30)(31)(32)(33)(34)(35).…”

Section: Significancementioning

confidence: 99%

Reducing posttreatment relapse in cleft lip palatal expansion using an injectable estrogen–nanodiamond hydrogel

Hong

Song

Lee

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Patients with cleft lip and/or palate (CLP), who undergo numerous medical interventions from infancy, can suffer from lifelong debilitation caused by underdeveloped maxillae. Conventional treatment approaches use maxillary expansion techniques to develop normal speech, achieve functional occlusion for nutrition intake, and improve esthetics. However, as patients with CLP congenitally lack bone in the cleft site with diminished capacity for bone formation in the expanded palate, more than 80% of the patient population experiences significant postexpansion relapse. While such relapse has been a long-standing battle in craniofacial care of patients, currently there are no available strategies to address this pervasive problem. Estrogen, 17β-estradiol (E2), is a powerful therapeutic agent that plays a critical role in bone homeostasis. However, E2's clinical application is less appreciated due to several limitations, including its pleiotropic effects and short half-life. Here, we developed a treatment strategy using an injectable system with photo-crosslinkable hydrogel (G) and nanodiamond (ND) technology to facilitate the targeted and sustained delivery of E2 to promote bone formation. In a preclinical expansion/relapse model, this functionalized E2/ ND/G complex substantially reduced postexpansion relapse by nearly threefold through enhancements in sutural remodeling compared with unmodified E2 administration. The E2/ND/G group demonstrated greater bone volume by twofold and higher osteoblast number by threefold, compared with the control group. The E2/ND/G platform maximized the beneficial effects of E2 through its extended release with superior efficacy and safety at the local level. This broadly applicable E2 delivery platform shows promise as an adjuvant therapy in craniofacial care of patients.nanomedicine | cleft palate | craniofacial medicine | nanodiamond | drug delivery

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Epirubicin-Adsorbed Nanodiamonds Kill Chemoresistant Hepatic Cancer Stem Cells

Cited by 180 publications

References 83 publications

Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization

Nanodiamonds coupled with 5,7-dimethoxycoumarin, a plant bioactive metabolite, interfere with the mitotic process in B16F10 cells altering the actin organization

Synthesis, characterisation and antimicrobial activity of manganese- and iron-doped zinc oxide nanoparticles

Reducing posttreatment relapse in cleft lip palatal expansion using an injectable estrogen–nanodiamond hydrogel

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