Epithelial and Neutrophil Interactions and Coordinated Response to<i>Shigella</i>in a Human Intestinal Enteroid-Neutrophil Coculture Model

Lemme-Dumit, Jose M.; Doucet, Michèle; Zachos, Nicholas C.; Pasetti, Marcela F.

doi:10.1128/mbio.00944-22

Cited by 14 publications

(14 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, investigations using advanced imaging have allowed scientists to visualize the nanoparticle pathway leading to and after internalization. Here, we summarize some of the main model systems used in the field and refer readers to several complete review articles that cover various models of the intestine, including animal and ex vivo, − 2D and 3D in vitro, ,, human enteroid, and microfluidic models …”

Section: Experimental Models To Study Nanoparticles’ Transport Mechan...mentioning

confidence: 99%

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

2023

View full text Add to dashboard Cite

Oral drug administration has been a popular choice due to patient compliance and limited clinical resources. Orally delivered drugs must circumvent the harsh gastrointestinal (GI) environment to effectively enter the systemic circulation.The GI tract has a number of structural and physiological barriers that limit drug bioavailability including mucus, the tightly regulated epithelial layer, immune cells, and associated vasculature. Nanoparticles have been used to enhance oral bioavailability of drugs, as they can act as a shield to the harsh GI environment and prevent early degradation while also increasing uptake and transport of drugs across the intestinal epithelium. Evidence suggests that different nanoparticle formulations may be transported via different intracellular mechanisms to cross the intestinal epithelium. Despite the existence of a significant body of work on intestinal transport of nanoparticles, many key questions remain: What causes the poor bioavailability of the oral drugs? What factors contribute to the ability of a nanoparticle to cross different intestinal barriers? Do nanoparticle properties such as size and charge influence the type of endocytic pathways taken? In this Review, we summarize the different components of intestinal barriers and the types of nanoparticles developed for oral delivery. In particular, we focus on the various intracellular pathways used in nanoparticle internalization and nanoparticle or cargo translocation across the epithelium. Understanding the gut barrier, nanoparticle characteristics, and transport pathways may lead to the development of more therapeutically useful nanoparticles as drug carriers.

show abstract

Section: Experimental Models To Study Nanoparticles’ Transport Mechan...mentioning

confidence: 99%

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

2023

View full text Add to dashboard Cite

show abstract

“…2D human intestinal enteroids (HIE) from primary tissue were used as an infection model, confirming preferential infection from M cells and the basolateral compartment ( Koestler et al., 2019 ; Llanos-Chea et al., 2019 ; Ranganathan et al., 2019 ). HIE can be co-cultured with PMNs to study the interplay of Shigella with respect to PMN-epithelial cell interaction ( Lemme-Dumit et al., 2022 ). Finally, 3D epithelial organoids derived from primary human tissue could be an interesting model for Shigella infection, especially apical-out organoids with reversed polarity would represent another useful model to investigate invasion on the apical vs. the basolateral side of the epithelium ( Co et al., 2021 ).…”

Section: Adhesion/invasion Inhibition Assaysmentioning

confidence: 99%

Functional assays to evaluate antibody-mediated responses against Shigella: a review

Boero

Vezzani

Micoli

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Shigella is a major global pathogen and the etiological agent of shigellosis, a diarrheal disease that primarily affects low- and middle-income countries. Shigellosis is characterized by a complex, multistep pathogenesis during which bacteria use multiple invasion proteins to manipulate and invade the intestinal epithelium. Antibodies, especially against the O-antigen and some invasion proteins, play a protective role as titres against specific antigens inversely correlate with disease severity; however, the context of antibody action during pathogenesis remains to be elucidated, especially with Shigella being mostly an intracellular pathogen. In the absence of a correlate of protection, functional assays rebuilding salient moments of Shigella pathogenesis can improve our understanding of the role of protective antibodies in blocking infection and disease. In vitro assays are important tools to build correlates of protection. Only recently animal models to recapitulate human pathogenesis, often not in full, have been established. This review aims to discuss in vitro assays to evaluate the functionality of anti-Shigella antibodies in polyclonal sera in light of the multistep and multifaced Shigella infection process. Indeed, measurement of antibody level alone may limit the evaluation of full vaccine potential. Serum bactericidal assay (SBA), and other functional assays such as opsonophagocytic killing assays (OPKA), and adhesion/invasion inhibition assays (AIA), are instead physiologically relevant and may provide important information regarding the role played by these effector mechanisms in protective immunity. Ultimately, the review aims at providing scientists in the field with new points of view regarding the significance of functional assays of choice which may be more representative of immune-mediated protection mechanisms.

show abstract

“…[129] Primary Immune Cells: Many intestinal in vitro models incorporate primary immune cell subpopulations, including lymphocytes, [130][131][132][133][134] monocytes/macrophages, [135,136] dendritic cells, [137] and neutrophils. [138,139] Tissue-resident immune cells can be harvested from the intestinal lamina propria and show tissue-specific characteristics and functions, being phenotypically different from the ones found in other tissues or in circulation and are thus more physiologically relevant. [67,132,137] Although scarce, resected tissue pieces remain the preferred option since biopsies often fail to provide sufficient cell numbers.…”

Section: Primary Cellsmentioning

confidence: 99%

“…This bidirectional relationship can be studied by coculturing immune cells with the epithelium. In this perspective, and due to performing distinct and specific functions in inflammation, different subsets of immune cells have been used in combination with organoids models: monocytes, [137] macrophages, [136,212] polymorphonuclear leukocytes, [138,139] lymphocytes, [130] and DCs. [141] Another drawback of organoids lies in their heterogeneous structures with a closed sphere-like spatial arrangement that prevents direct access to the apical side of the epithelium.…”

Section: Organoidsmentioning

confidence: 99%

Recent Advances in Cell‐Based In Vitro Models to Recreate Human Intestinal Inflammation

Macedo,

Dias Neto,

Pastrana

et al. 2023

Advanced Science

View full text Add to dashboard Cite

Inflammatory bowel disease causes a major burden to patients and healthcare systems, raising the need to develop effective therapies. Technological advances in cell culture, allied with ethical issues, have propelled in vitro models as essential tools to study disease aetiology, its progression, and possible therapies. Several cell‐based in vitro models of intestinal inflammation have been used, varying in their complexity and methodology to induce inflammation. Immortalized cell lines are extensively used due to their long‐term survival, in contrast to primary cultures that are short‐lived but patient‐specific. Recently, organoids and organ‐chips have demonstrated great potential by being physiologically more relevant. This review aims to shed light on the intricate nature of intestinal inflammation and cover recent works that report cell‐based in vitro models of human intestinal inflammation, encompassing diverse approaches and outcomes.

show abstract

Epithelial and Neutrophil Interactions and Coordinated Response toShigellain a Human Intestinal Enteroid-Neutrophil Coculture Model

Cited by 14 publications

References 78 publications

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

Functional assays to evaluate antibody-mediated responses against Shigella: a review

Recent Advances in Cell‐Based In Vitro Models to Recreate Human Intestinal Inflammation

Contact Info

Product

Resources

About