Epithelium and Stroma in Alkali-Burned Corneas

Effect of 0.1% dexamethasone on epithelial healing in experimental corneal alkali wounds: morphological changes during the repair process

“…In agreement with Matsuda and Smelser [20], we observed multiple electron-dense particles which were mostly dispersed into the anterior stroma. These electrondense particles were scattered in the area just below the empty epithelial cell space that might have originated from the ruptured basal cell.…”

“…An intact basement membrane, which provides a firm attachment for basal epithelial cells to the stroma, might be crucial for maintenance of the corneal integrity in alkali wound healing [14,15,18,29]. Despite many therapeutic trials, the fate of basement membrane following alkali damage has not been detailed [20,24].…”

Stem cells and corneal epithelial regeneration

“…II I . [114][115][116] In contrast to minor injuries in which a loss of corneal epithelium is combined with minor limbal damage, larger defects of the limbal cir cumference cannot heal by sliding of the adj acent healthy limbal epithelium.lo In these cases, and after injuries in which severe inflammation resulted in regulatory dys function of the limbal epithelium, a localised loss of the limbal barrier occurs with consecutive invasion of con junctival epithelium. 10 , 1 5 .…”

Type IV Collagen in the Basement Membrane of the Corneal Epithelium after Alkali Burns in Guinea Pigs

“…After burning of the central cornea with alkali, the BM is degraded by matrix metallo proteinases secreted by the regenerated cor neal cells [1][2][3][4][5][6]. We previously reported that type IV collagen immunoreactivity tran siently develops during the reconstruction of the BM after such injury [5],…”