Epithelium regeneration on collagen (IV) grafted polycaprolactone for esophageal tissue engineering

Zhu, Yabin; Ong, Wey Feng

doi:10.1016/j.msec.2008.09.004

Cited by 27 publications

(28 citation statements)

References 18 publications

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“…Esophagus consists of four layers that comprise three different kinds of cells -ECs, FBs and SMCs. [24] The innermost layer of esophagus is the mucosal layer having ECs, which protects the esophagus from the mechanical stress caused by the food bolus through the secretion of mucus. [32] Therefore ECs could be important for esophageal functions.…”

Section: Discussionmentioning

confidence: 99%

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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-based nanofibrous scaffolds to support functional esophageal epithelial cells towards engineering the esophagus

Kuppan

Sethuraman

Krishnan

2014

Journal of Biomaterials Science, Polymer Edition

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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and PHBV-gelatin were electrospun to obtain defect-free nanofibers by optimizing various process and solution parameters. Tensile strength, Young's modulus, and wettability of PHBV-gelatin nanofibrous scaffold were determined and compared with PHBV nanofibrous scaffold. Our results demonstrate that PHBV-gelatin nanofibers exhibited higher tensile strength and Young's modulus than the PHBV nanofibers. Human esophageal epithelial cells (HEEpiC) were cultured on PHBV and PHBV-gelatin nanofiber showed better cell proliferation in PHBV nanofibrous scaffold than the PHBV-gelatin scaffold after 7 days of culture. HEEpiC cultured on PHBV and PHBV-gelatin nanofibrous scaffold exhibited characteristic epithelial cobblestone morphology after 3 days of culture. Further, the HEEpiC extracellular matrix (ECM) proteins (collagen type IV and laminin) and phenotypic marker proteins (cytokeratin-4 and 14) expressions were significantly higher in PHBV-gelatin nanofibrous scaffold than the PHBV nanofiber scaffold. However, the long-term stability and functional state of the cells on the PHBV scaffold give it an edge over the blend scaffolds. Thus, PHBV-based nanofibrous scaffolds could be explored further as ECM substitutes for the regeneration of esophageal tissue.

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Section: Discussionmentioning

confidence: 99%

“…Esophagus is a complex organ that consists of multiple layers of ECs, fibroblast cells (FBs) and smooth muscle cells (SMCs). [24] Tissue engineered scaffold should support the growth of both epithelial and SMCs to support the native functions of esophagus. Research work on the decellularized ECM of esophagus, urinary bladder matrices etc.…”

Section: Introductionmentioning

confidence: 99%

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-based nanofibrous scaffolds to support functional esophageal epithelial cells towards engineering the esophagus

Kuppan

Sethuraman

Krishnan

2014

Journal of Biomaterials Science, Polymer Edition

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“…The PCL-collagen composite accelerated the regeneration of the epithelium, cell spreading and interconnection between cells. Immunostaining studies confirmed the epithelial phenotype of the cells [36]. …”

Section: - Tissue Engineering Of the Gi Tract: Esophagus Stomach Smentioning

confidence: 79%

Tissue engineering and regenerative medicine as applied to the gastrointestinal tract

Bitar

Zakhem

2013

Current Opinion in Biotechnology

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The gastrointestinal (GI) tract is a complex system characterized by multiple cell types with a determined architectural arrangement. Tissue engineering of the GI tract aims to reinstate the architecture and function of all structural layers. The key point for successful tissue regeneration includes the use of cells/biomaterials that elucidate minimal immune response after implantation. Different biomaterial choices and cell sources have been proposed to engineer the GI tract. This review summarizes the recent advances in bioengineering the GI tract with emphasis on cell sources and scaffolding biomaterials.

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“…58 Electrospun poly(l-lactide-co-caprolactone) (PLLC) scaffolds were immobilized with fibronectin as an attempt to enhance cell attachment. Synthetic materials display strong biocompatibility and biodegradability properties.…”

Section: Synthetic Materialsmentioning

confidence: 99%

Tissue Engineering and Regenerative Medicine

Zakhem¹,

Bitar²

2016

Translating Regenerative Medicine to the Clinic

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Epithelium regeneration on collagen (IV) grafted polycaprolactone for esophageal tissue engineering

Cited by 27 publications

References 18 publications

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-based nanofibrous scaffolds to support functional esophageal epithelial cells towards engineering the esophagus

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-based nanofibrous scaffolds to support functional esophageal epithelial cells towards engineering the esophagus

Tissue engineering and regenerative medicine as applied to the gastrointestinal tract

Tissue Engineering and Regenerative Medicine

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