Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-based nanofibrous scaffolds to support functional esophageal epithelial cells towards engineering the esophagus

Abstract

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and PHBV-gelatin were electrospun to obtain defect-free nanofibers by optimizing various process and solution parameters. Tensile strength, Young's modulus, and wettability of PHBV-gelatin nanofibrous scaffold were determined and compared with PHBV nanofibrous scaffold. Our results demonstrate that PHBV-gelatin nanofibers exhibited higher tensile strength and Young's modulus than the PHBV nanofibers. Human esophageal epithelial cells (HEEpiC) were cultured on PHBV and PHBV-gelatin nanofiber showed better cell proliferation in PHBV nanofibrous scaffold than the PHBV-gelatin scaffold after 7 days of culture. HEEpiC cultured on PHBV and PHBV-gelatin nanofibrous scaffold exhibited characteristic epithelial cobblestone morphology after 3 days of culture. Further, the HEEpiC extracellular matrix (ECM) proteins (collagen type IV and laminin) and phenotypic marker proteins (cytokeratin-4 and 14) expressions were significantly higher in PHBV-gelatin nanofibrous scaffold than the PHBV nanofiber scaffold. However, the long-term stability and functional state of the cells on the PHBV scaffold give it an edge over the blend scaffolds. Thus, PHBV-based nanofibrous scaffolds could be explored further as ECM substitutes for the regeneration of esophageal tissue.

Osteogenic differentiation of stem cells on mesoporous silica nanofibers

“…The proliferation of cells was determined aer 1, 3 & 10 days using MTS assay (CellTiter 96 AQueous one solution, Promega, USA). 25 At the end of each time point, the samples were washed with D-PBS solution to remove the non-adherent cells. Serumfree media (1 mL) and 200 mL of MTS reagent were added to each well and incubated at 37 C for 2 hours.…”

Fish scale/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofibrous composite scaffolds for bone regeneration

“…32 Therefore, PHBV can be used in combination with other polymers such as PLLA, gelatin, collagen, chitosan, and silk to enhance degradability, 26,30 hydrophilicity, and biocompatibility. 26,30,33–36…”

Effect of different solvent systems on PHBV/PEO electrospun fibers

“…Meng et al 16 dissolved PHBV in hexauoroisopropanol (HFIP), the ber diameter were 600 nm, and the cell culture experiments indicated that the PHBV nanobrous scaffold accelerated the adhesion and growth of NIH3T3 cells, thus making the former a good scaffold for tissue engineering. Kuppan et al 11,14 dissolved PHBV in a mixture of DCM and DMF (9 : 1) to form a 15% (w/v) solution. The average diameter of the PHBV bers was 724 nm.…”