Fish scale/poly(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate) nanofibrous composite scaffolds for bone regeneration

Kara, Aylin; Güneş, Oylum Çolpankan; Albayrak, Aylin Ziylan; Bilici, Gökçen; Erbil, Güven; Havıtçıoğlu, Hasan

doi:10.1177/0885328220901987

Cited by 12 publications

(10 citation statements)

References 46 publications

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“…In a typical experiment, fish scales from tilapia were first decellularized with Tris-HCl buffer containing 0.1% SDS for 3 days [ 18 , 19 ]. After decellularization, fish scales were decalcified with formic acid for 3 days, and then DDFSs were obtained.…”

Section: Resultsmentioning

confidence: 99%

Self-curling 3D oriented scaffolds from fish scales for skeletal muscle regeneration

Shi

Zhang

et al. 2022

Biomater Res

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Background Volumetric muscle loss (VML) due to various reasons may cause motor dysfunction and tissue engineering has been proposed for muscle regeneration. However, developing three-dimensional (3D) tissue-engineered scaffolds that can mimic oriented cell growth of muscle tissues are challenging for regeneration medicine. Herein, we propose a novel self-curling 3D oriented scaffold (SCOS) composed of fish derived gelatin methacrylate (GelMA) and fish scales for repairing skeletal muscles. Methods Fish scales of tilapia were decellularized and decalcified. Then, SCOSs were constructed by ultraviolet-coating methylated fish gelatin on the back of fish scales. C2C12 myoblasts were cultured on SCOSs, and after induction of myogenic differentiation, SCOS/C2C12 transplants were prepared for in vivo experiments. Results Decellularized and decalcified fish scales (DDFSs) became soft and retained the original oriented microgroove surface structure that could induce oriented cell growth. SCOSs could self-curl into 3D structures when immersing in culture medium due to different swelling properties of fish GelMA and DDFSs. Cell experiments demonstrated that SCOSs enhanced the oriented growth and myogenic differentiation of C2C12 myoblasts. By integrating SCOSs and myogenic differentiated C2C12 myoblasts, the resultant SCOS/C2C12 transplants promoted de novo muscle regeneration and functional restoration of muscle activity in the mouse model of VML. Conclusions Our results suggest that SCOSs loaded with myogenic differentiated C2C12 myoblasts can promote muscle regeneration in mice with skeletal muscle injuries, indicating application prospects of such scaffolds in muscle tissue engineering and other related fields.

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Section: Resultsmentioning

confidence: 99%

Self-curling 3D oriented scaffolds from fish scales for skeletal muscle regeneration

Shi

Zhang

et al. 2022

Biomater Res

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“…Influence of the environment on the biological cells of the fish scales was revealed The structures of the constituent fibers were revealed Soleidae flatfish [44] XRD, DSC, polarized light microscopy, flow measurement Biochemical and biophysical properties were analyzed Sparus aurata [19,[46][47][48] FTIR, TG-DTA analysis, SDS-PAGE analysis, polarized light microscopy…”

Section: Fish Experimental Methods Key Discoveriesmentioning

confidence: 99%

“…This study, like a similar earlier study [45], was concerned with the biomedical application of fish scales from an amino acid perspective. Further studies have also been carried out focusing on the biocompatibility and potential bone regenerative properties of fish scales, such as those of Sparus aurata [19,[46][47][48]. Similarly, fish scales and decalcified scaffolds collected from crisp flesh grass carp were tested using SEM, FTIR, energy-dispersive x-ray spectroscopy (EDX or EDS) and tensile testing.…”

Section: Mechanical Characterization Of Natural Fish Scalesmentioning

confidence: 99%

Fish scale inspired structures—a review of materials, manufacturing and models

Hossain¹,

Ebrahimi²,

Ghosh³

2022

Bioinspir. Biomim.

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Fish scales inspired materials platform can provide advanced mechanical properties and functionalities. These materials, inspired from fish scales take the form of either composite materials or multi-material discrete exoskeleton type structures. Over the last decade, they have been under intense scrutiny for generating tailorable and tunable stiffness, penetration and fracture resistance, buckling prevention, nonlinear damping, hydrodynamic and camouflaging functions. Such programmable behavior emerges from leveraging their unique morphology and structure-property relationships. Several advanced tools of characterization, manufacturing, modeling and computation have been employed to understand and discover their behavior. With the rapid proliferation of additive manufacturing (AM) techniques, and advancing envelope of modeling and computational methods, this field is seeing renewed efforts to realize even more ambitious designs. We present a review and recapitulation of the state-of-the art in fish scale inspired materials in this paper.

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“…2020, produced 3D nanofibrous fish scale/poly (3-hydroxybutyrate-co-3hydroxyvalerate) (PHBV) (Mw: 80.000 Da) 3 % w/v, including an organic soluble salt BTEAC 0.2 % w/v dissolved in chloroform, collected in ethanol-water (9:1 v: v) as coagulant. The incorporation of fish scales into the PHBV scaffold increased biomineralization the deposition of Ca-P minerals, as well as the compressive modulus and higher bioactivity, MG-63 cell viability, alkaline phosphatase activity, and type I collagen production [57]. Dalgic et.…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

Wet Electrospinning and its Applications: A Review

Suaza

Henao

2022

TecnoL.

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In wet electrospinning, a natural or synthetic polymer solution is deposited on a non-solvent liquid coagulant used as collector. This technique can create 3D nanofiber scaffolds with better properties (e.g., porosity and high surface area) than those of traditional 2D scaffolds produced by standard electrospinning. Thanks to these characteristics, wet electrospinning can be employed in a wide range of tissue engineering and industrial applications. This review aims to broaden the panorama of this technique, its possible fields of action, and its range of common materials. Moreover, we also discuss its future trends. In this study, we review papers on this method published between 2017 and 2021 to establish the state of the art of wet electrospinning and its most important applications in cardiac, cartilage, hepatic, wound dressing, skin, neural, bone, and skeletal muscle tissue engineering. Additionally, we examine its industrial applications in water purification, air filters, energy, biomedical sensors, and textiles. The main results of this review indicate that 3D scaffolds for tissue engineering applications are biocompatible; mimic the extracellular matrix (ECM); allow stem cell viability and differentiation; and have high porosity, which provides greater cell infiltration compared to 2D scaffolds. Finally, we found that, in industrial applications of wet electrospinning: (1) additives improve the performance of pure polymers; (2) the concentration of the solution influences porosity and fiber packing; (3) flow rate, voltage, and distance modify fiber morphology; (4) the surface tension of the non-solvent coagulant on which the fibers are deposited has an effect on their porosity, compaction, and mechanical properties; and (5) deposition time defines scaffold thickness.

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Fish scale/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofibrous composite scaffolds for bone regeneration

Cited by 12 publications

References 46 publications

Self-curling 3D oriented scaffolds from fish scales for skeletal muscle regeneration

Self-curling 3D oriented scaffolds from fish scales for skeletal muscle regeneration

Fish scale inspired structures—a review of materials, manufacturing and models

Wet Electrospinning and its Applications: A Review

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