Epitope-Based Peptide Vaccine Against Fructose-Bisphosphate Aldolase of Madurella mycetomatis Using Immunoinformatics Approaches

Abstract

Background:Mycetoma is a distinct body tissue destructive and neglected tropical disease. It is endemic in many tropical and subtropical countries. Mycetoma is caused by bacterial infections (actinomycetoma) such as Streptomyces somaliensis and Nocardiae or true fungi (eumycetoma) such as Madurella mycetomatis. To date, treatments fail to cure the infection and the available marketed drugs are expensive and toxic upon prolonged usage. Moreover, no vaccine was prepared yet against mycetoma.Aim:The aim of this study is to predict effective epitope-based vaccine against fructose-bisphosphate aldolase enzymes of M. mycetomatis using immunoinformatics approaches.Methods and materials:Fructose-bisphosphate aldolase of M. mycetomatis sequence was retrieved from NCBI. Different prediction tools were used to analyze the nominee’s epitopes in Immune Epitope Database for B-cell, T-cell MHC class II and class I. Then the proposed peptides were docked using Autodock 4.0 software program.Results and conclusions:The proposed and promising peptides KYLQ show a potent binding affinity to B-cell, FEYARKHAF with a very strong binding affinity to MHC I alleles and FFKEHGVPL that shows a very strong binding affinity to MHC II and MHC I alleles. This indicates a strong potential to formulate a new vaccine, especially with the peptide FFKEHGVPL which is likely to be the first proposed epitope-based vaccine against fructose-bisphosphate aldolase of M. mycetomatis. This study recommends an in vivo assessment for the most promising peptides especially FFKEHGVPL.

Epitope-Based Peptide Vaccine against Glycoprotein G of Nipah Henipavirus Using Immunoinformatics Approaches

“…However, novel vaccine approaches such as DNA-and epitope-based immunizations may possibly conquer obstructions for this type of immunization approaches, making them increasingly successful, explicit, and longlasting in vulnerable reactions with insignificant structures and without any undesired impacts [46]. Moreover, many peptide-based vaccines have been effectively proposed through utilizing in silico approaches against Madurella mycetomatis, Mokola rabies virus, Lagos rabies virus, and others [47][48][49][50][51][52]. Such investigations, in regard to those viruses, have built up immunoinformatics in the computational analysis field.…”

Epitope - based peptide vaccine against glycoprotein G of Nipah henipavirus using immunoinformatics approaches

“…However, novel vaccines approach as epitope-based peptide vaccines can possibly conquer these obstructions for these immunizations can make increasingly successful, specific and long lasting impregnable reaction and without all the adverse effects of the classical vaccines. Moreover, based on our previous studies peptide-based vaccines have been effectively proposed by utilizing in silico approach against Madurella mycetomatis, Mokola Rabies Virus, Lagos Rabies Virus and others [49,50,51] . Such investigations in regards to those viruses built up immunoinformatics considered as a computational analysis.…”

Design of Epitope Based Peptide Vaccine Against Pseudomonas Aeruginosa Fructose Bisphosphate Aldolase Protein using Immunoinformatics

“…Many studies had predicted peptide vaccines for different microorganisms such as, Rubella, Ebola, Dengue, Zika, HPV, Lagos rabies virus, and mycetoma using immunoinformatics tools. [44][45][46][47][48][49][50][51][52][53] Limitations include the exclusion of certain HLA alleles for the MHC II. We hope that the whole world will benefit from this epitope-based vaccine and recommend invivo and invitro studies to prove it's effectiveness.…”