Epitope-specific immune recognition of the nontypeable<i>Haemophilus influenzae</i>outer membrane protein 26

Kunthalert, Duangkamol; Novotny, Laura A.; Massa, Helen Maureen; Ulett, Glen C.; Kyd, Jennelle M.; Cripps, Allan W.

doi:10.4161/hv.23255

Cited by 5 publications

(4 citation statements)

References 40 publications

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“…Epitope-based vaccines have for long stood out as promising vaccine candidates against diverse pathogens (Christy et al 2012;Hurtgen et al 2012;Pan et al 2012;Kunthalert et al 2013;Rojas-Caraballo et al 2014) and have forayed their way in human clinical trials as well (Iinuma et al 2014). Other than helping to focus on and thereby narrowing down the specificity of immune response, they also leave room for chimera generation including multiple epitopes from same as well as different and slightly more immunogenic antigens.…”

Section: Discussionmentioning

confidence: 97%

Identification and immunogenic potential of B cell epitopes of outer membrane protein OmpF of Aeromonas hydrophila in translational fusion with a carrier protein

Sharma

Dixit

2015

Appl Microbiol Biotechnol

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Aeromonas hydrophila, a ubiquitous and virulent bacterial pathogen, affects a variety of fishes, including Labeo rohita. Existing treatment strategies comprise antibiotic therapies and attenuated bacterial strain-based vaccines. No functional subunit vaccine has been available until now. Given their key role in determining pathogenicity, outer membrane proteins have been successfully explored as potential vaccine candidates. We have devised a direct strategy for eliminating non-specific responses by selectively aiming the immune response against specific immunodominant epitopes of the outer membrane protein F (OmpF) of A. hydrophila (AhOmpF). Five putative epitopes of AhOmpF predicted in silico were genetically conjugated with heat labile enterotoxin chain B of E. coli (LTB). Recombinant fusion proteins expressed in E. coli were purified from solubilized inclusion bodies and refolded. The fusion protein retained GM1 ganglioside receptor binding activity of LTB, indicating proper folding. Four of the five fusion proteins were found to be highly immunogenic. Of the four proteins, antisera against the fusion protein (anti-rEpiF1) harboring 66-80 amino acid residues of the OmpF gave maximum cross-reactivity with the targeted rOmpF in enzyme-linked immunosorbent assay (ELISA) and was able to recognize both fusion partners-rOmpF and rLTB-in Western blot. Antibody isotyping of the antisera and cytokine array analysis of the culture supernatants of splenocytes from sensitized mice manifested a mixed Th1/Th2 immune response with a bias toward Th2. Anti-rEpiF1 antibodies were able to bind to the cell membrane of live A. hydrophila cells and agglutinate them. Our results thus suggest that the OmpF epitope (66-80) in fusion with a carrier protein is a promising vaccine candidate against A. hydrophila.

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Section: Discussionmentioning

confidence: 97%

Identification and immunogenic potential of B cell epitopes of outer membrane protein OmpF of Aeromonas hydrophila in translational fusion with a carrier protein

Sharma

Dixit

2015

Appl Microbiol Biotechnol

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“…87 Recent work defined outer membrane protein 26 T- and B-cell epitopes, which will improve existing NTHi vaccines. 88 …”

Section: Progress In Identification Of New Vaccine Candidatesmentioning

confidence: 99%

“…87 Recent work defined outer membrane protein 26 T-and B-cell epitopes, which will improve existing NTHi vaccines. 88 Winter and coworkers examined the ability of antisera raised against purified high molecular weight (HMW) proteins HMW1/HMW2 or recombinant Hia proteins to mediate opsonophagocytic killing of a large panel of NTHi strains. 89 The 3 HMW1/HMW2 antisera mediated killing of 48 of 65 HMW1/HMW2-expressing strains, and the 2 Hia antisera mediated killing of 15 of 24 Hia-expressing strains.…”

Section: Nontypeable H Influenzaementioning

confidence: 99%

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Panel 6: Vaccines

Pettigrew

Alderson

Barenkamp

et al. 2017

Otolaryngol.--head neck surg.

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Objective To review the literature on progress regarding (1) effectiveness of vaccines for prevention of otitis media (OM) and (2) development of vaccine antigens for OM bacterial and viral pathogens. Data Sources PubMed database of the National Library of Science. Review Methods We performed literature searches in PubMed for OM pathogens and candidate vaccine antigens, and we restricted the searches to articles in English that were published between July 2011 and June 2015. Panel members reviewed literature in their area of expertise. Conclusions Pneumococcal conjugate vaccines (PCVs) are somewhat effective for the prevention of pneumococcal OM, recurrent OM, OM visits, and tympanostomy tube insertions. Widespread use of PCVs has been associated with shifts in pneumococcal serotypes and bacterial pathogens associated with OM, diminishing PCV effectiveness against AOM. The 10-valent pneumococcal vaccine containing Haemophilus influenzae protein D (PHiD-CV) is effective for pneumococcal OM, but results from studies describing the potential impact on OM due to H influenzae have been inconsistent. Progress in vaccine development for H influenzae, Moraxella catarrhalis, and OM-associated respiratory viruses has been limited. Additional research is needed to extend vaccine protection to additional pneumococcal serotypes and other otopathogens. There are likely to be licensure challenges for protein-based vaccines, and data on correlates of protection for OM vaccine antigens are urgently needed. Implications for Practice OM continues to be a significant health care burden globally. Prevention is preferable to treatment, and vaccine development remains an important goal. As a polymicrobial disease, OM poses significant but not insurmountable challenges for vaccine development.

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Development and antigenic characterization of three recombinant proteins with potential for Glässer's disease prevention

Song

et al. 2016

Vaccine

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Epitope-specific immune recognition of the nontypeableHaemophilus influenzaeouter membrane protein 26

Cited by 5 publications

References 40 publications

Identification and immunogenic potential of B cell epitopes of outer membrane protein OmpF of Aeromonas hydrophila in translational fusion with a carrier protein

Identification and immunogenic potential of B cell epitopes of outer membrane protein OmpF of Aeromonas hydrophila in translational fusion with a carrier protein

Panel 6: Vaccines

Development and antigenic characterization of three recombinant proteins with potential for Glässer's disease prevention

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