Epizootic Hemorrhagic Disease Virus and Bluetongue Virus Seroprevalence in Wild White-Tailed Deer (Odocoileus virginianus) in Florida, USA

Uribasterra, Maria; Orange, Jeremy P.; Dinh, Emily T. N.; Peters, C J; Peters, Rebecca; Goodfriend, Olivia; Blackburn, Jason K.

doi:10.7589/2019-10-263

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…Importantly, however, this does not mean that segments 2 and 6 of the Tunisian strain have an Australian origin as geographic sampling of these viruses is so limited, with only a few available sequences available for most years. Indeed, it is striking that the most recent sequences deposited on NCBI prior to 2021 are isolates from Florida from 2019, sampled during an outbreak in whitetail deer [35]. In addition, only one other sequence was available from Tunisia, serotype 6 from 2006, and only four sequences were available from other African countries.…”

Section: Discussionmentioning

confidence: 99%

Epizootic Haemorrhagic Disease Virus Serotype 8 in Tunisia, 2021

Sghaier¹,

Sailleau²,

Marcacci³

et al. 2022

Preprint

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Epizootic haemorrhagic disease (EHD) is a Culicoides-borne viral disease caused by epizootic haemorrhagic disease virus (EHDV) and associated with clinical manifestations in cervids and bovids. In late September 2021, EHDV was reported in cattle farms in central/western Tunisia. It rapidly spread throughout the country with more than 200 confirmed outbreaks. A combination of classical and molecular techniques was applied to characterize the causative virus as a member of EHDV-8 serotype. This is the first evidence of EHDV- 8 circulation since 1982 when the prototype EHDV-8 strain was isolated in Australia. This work highlights the urgent need for vaccines for a range of EHDV serotypes.

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Section: Discussionmentioning

confidence: 99%

Epizootic Haemorrhagic Disease Virus Serotype 8 in Tunisia, 2021

Sghaier¹,

Sailleau²,

Marcacci³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Importantly, however, this does not mean that segments 2 and 6 of the Tunisian strain have an Australian origin, as the geographic sampling of these viruses is so limited, with only a few available sequences available for most years. Indeed, it is striking that the most recent sequences deposited on NCBI prior to 2021 are isolates from Florida from 2019, sampled during an outbreak in white-tail deer [ 34 ]. In addition, only one other sequence was available from Tunisia, serotype 6 from 2006, and only four sequences were available from other African countries.…”

Section: Discussionmentioning

confidence: 99%

Epizootic Haemorrhagic Disease Virus Serotype 8 in Tunisia, 2021

Sghaier¹,

Sailleau

Marcacci

et al. 2022

Viruses

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Epizootic haemorrhagic disease (EHD) is a Culicoides-borne viral disease caused by the epizootic haemorrhagic disease virus (EHDV) associated with clinical manifestations in domestic and wild ruminants, primarily white-tailed deer (Odocoileus virginianus) and cattle (Bos taurus). In late September 2021, EHDV was reported in cattle farms in central/western Tunisia. It rapidly spread throughout the country with more than 200 confirmed outbreaks. We applied a combination of classical and molecular techniques to characterize the causative virus as a member of the serotype EHDV-8. This is the first evidence of EHDV- 8 circulation since 1982 when the prototype EHDV-8 strain was isolated in Australia. This work highlights the urgent need for vaccines for a range of EHDV serotypes.

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“…In Florida, multiple orbiviruses have been isolated from diseased WTD on farms including Big Cypress orbivirus, bluetongue virus (BTV), CHeRI orbiviruses 1–3, epizootic hemorrhagic disease virus (EHDV) serotypes 1, 2, and 6, mobuck orbivirus, and Yunnan orbivirus [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. EHDV is widely distributed among farmed WTD in Florida.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (Odocoileus virginianus) on a Florida Farm

Viadanna

Subramaniam

Hawkins

et al. 2022

Viruses

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We report an outbreak of a novel reassortant epizootic hemorrhagic disease virus serotype 6 (EHDV-6) in white-tailed deer (WTD) on a Florida farm in 2019. At necropsy, most animals exhibited hemorrhagic lesions in the lung and heart, and congestion in the lung, liver, and spleen. Histopathology revealed multi-organ hemorrhage and congestion, and renal tubular necrosis. Tissues were screened by RT-qPCR and all animals tested positive for EHDV. Tissues were processed for virus isolation and next-generation sequencing was performed on cDNA libraries generated from the RNA extracts of cultures displaying cytopathic effects. Six isolates yielded nearly identical complete genome sequences of a novel U.S. EHDV-6 strain. Genetic and phylogenetic analyses revealed the novel strain to be most closely related to a reassortant EHDV-6 strain isolated from cattle in Trinidad and both strains received segment 4 from an Australian EHDV-2 strain. The novel U.S. EHDV-6 strain is unique in that it acquired segment 8 from an Australian EHDV-8 strain. An RNAscope® in situ hybridization assay was developed against the novel U.S. EHDV-6 strain and labeling was detected within lesions of the heart, kidney, liver, and lung. These data support the novel U.S. reassortant EHDV-6 strain as the cause of disease in the farmed WTD.

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Epizootic Hemorrhagic Disease Virus and Bluetongue Virus Seroprevalence in Wild White-Tailed Deer (Odocoileus virginianus) in Florida, USA

Cited by 6 publications

References 15 publications

Epizootic Haemorrhagic Disease Virus Serotype 8 in Tunisia, 2021

Epizootic Haemorrhagic Disease Virus Serotype 8 in Tunisia, 2021

Epizootic Haemorrhagic Disease Virus Serotype 8 in Tunisia, 2021

Characterization of a Novel Reassortant Epizootic Hemorrhagic Disease Virus Serotype 6 Strain Isolated from Diseased White-Tailed Deer (Odocoileus virginianus) on a Florida Farm

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