EPS production by
            <i>Propionibacterium freudenreichii</i>
            facilitates its immobilization for propionic acid production

Belgrano, Fabricio S.; Verçoza, Brunno Renato Farias; Rodrigues, Juliany Cola Fernandes; Hatti‐Kaul, Rajni; Pereira, Nei

doi:10.1111/jam.13895

Cited by 13 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Free cell yield decreased throughout the fermentation cycles. These results suggest that P. acidipropionici might produce an exopolysaccharide (EPS), similar to P. freudenreichii, which was recently reported as an EPS-producing strain for improving cell immobilization in PA production [37].…”

Section: Figsupporting

confidence: 71%

Improving propionic acid production from a hemicellulosic hydrolysate of sorghum bagasse by means of cell immobilization and sequential batch operation

Castro

Maeda

Rocha

et al. 2020

Biotechnology and Applied Biochemistry

Self Cite

View full text Add to dashboard Cite

Propionic acid (PA) is an important organic compound with extensive application in different industrial sectors and is currently produced by petrochemical processes. The production of PA by large-scale fermentation processes presents a bottleneck, particularly due to low volumetric productivity. In this context, the present work aimed to produce PA by a biochemical route from a hemicellulosic hydrolysate of sorghum bagasse using the strain Propionibacterium acidipropionici CIP 53164. Conditions were optimized to increase volumetric productivity and process efficiency. Initially, in simple batch fermentation, a final concentration of PA of 17.5 gL −1 was obtained. Next, fed batch operation with free cells was adopted to minimize substrate inhibition. Although a higher concentration of PA was achieved (38.0 gL −1 ), the response variables (Y P/S = 0.409 gg −1 and Q P = 0.198 gL −1 H −1 ) were close to those of the simple batch experiment. Finally, the fermentability of the hemicellulosic hydrolysate was investigated in a sequential batch with immobilized cells. The PA concentration achieved a maximum of 35.3 gL −1 in the third cycle; moreover, the volumetric productivity was almost sixfold higher (1.17 gL −1 H −1 ) in sequential batch than in simple batch fermentation. The results are highly promising, providing preliminary data for studies on scaling up the production of this organic acid.

show abstract

Section: Figsupporting

confidence: 71%

Improving propionic acid production from a hemicellulosic hydrolysate of sorghum bagasse by means of cell immobilization and sequential batch operation

Castro

Maeda

Rocha

et al. 2020

Biotechnology and Applied Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Adenosylcobalamin is coproduced by P. freudenreichii together with the main product propionic acid (PA). Many studies attempted to increase the production of PA and VB 12 in Propionibacterium (Belgrano et al, 2018;Suwannakham & Yang, 2005). Several studies had attempted to increase VB 12 production by adding some precursors such as cobalt ions (Quesada-Chanto et al, 1994;Seidametova et al, 2004;Yongsmith et al, 1982) and DMBI (Marwaha & Sethi, 1984).…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling analysis of the vitamin B₁₂ producer Propionibacterium freudenreichii

Liu

et al. 2021

MicrobiologyOpen

View full text Add to dashboard Cite

Vitamin B 12 (VB 12 ) is an indispensable cofactor of metabolic enzymes and has been widely used in the food and pharmaceutical industries. In this study, the effects of medium composition on VB 12 production by Propionibacterium freudenreichii were evaluated and optimized based on statistical experiments. The results showed that glucose, yeast extract, KH 2 PO 4 , and glycine have significant effects on VB 12 production. The final titer of VB 12 reached 8.32 ± 0.02 mg/L, representing a 120% increase over the non‐optimized culture medium. We employed a metabolomics approach to analyze the differences of metabolite concentrations in P . freudenreichii cells cultivated in the original medium and optimized fermentation medium. Using multivariate data analysis, we identified a range of correlated metabolites, illustrating how metabolomics can be used to explain VB 12 production changes by corresponding differences in the overall cellular metabolism. The concentrations of many metabolic intermediates of glycolysis, the Wood–Werkman cycle, the TCA cycle, and amino acid metabolism were increased, which contributed to the synthesis of propionic acid and VB 12 due to an improved supply of energy and precursors.

show abstract

“…The viscosity of the growth medium has been speculated to be associated with EPS (Cerning, 1990). Recently, it was found with P. freundreichii that EPS production was affected by medium composition and fermentation conditions (Belgrano et al, 2018). P. freudenreichii subsp.…”

Section: Propionibacterium Ruminifibrarum: Its Potential Role In Fibrmentioning

confidence: 99%

“…P. freudenreichii subsp. shermanii strain JS, which belongs to the dairy group of propionibacteria, also produces large quantities of EPS (Belgrano et al, 2018).…”

Section: Propionibacterium Ruminifibrarum: Its Potential Role In Fibrmentioning

confidence: 99%

Assessing rumen microbial composition and fibre attachment in dairy cows

Vaidya¹

View full text Add to dashboard Cite

Other natural sources 199 (104-297) Other land sources 185 (99-272) Freshwaters 122 (60-180) Geological (onshore) 40 (30-56) Wild animals 10 (5-15) Termites 9 (3-15) Wildfires 3 (1-5) Permafrost soils (direct) 1 (0-1) Oceanic sources 14 (5-25) Geological (offshore) 12 (5-20) Total anthropogenic sources Agriculture and waste 195 (178-206) Enteric fermentation and manure 106 (97-111) Landfills and waste 59 (52-63) Rice cultivation 30 (24-36) Fossil fuels 121 (114-133) Coal mining 41 (26-50) Gas, oil, and industry 79 (69-88) Biomass and biofuel burning 30 (27-35)

show abstract

EPS production by Propionibacterium freudenreichii facilitates its immobilization for propionic acid production

Abstract: This study demonstrates the EPS-producing microbe to be easily immobilized on a solid matrix and to be used in a bioprocess. Such a system could be optimized for achieving high cell density in fermentations without the need for functionalization of the matrix.

Cited by 13 publications

References 36 publications

Improving propionic acid production from a hemicellulosic hydrolysate of sorghum bagasse by means of cell immobilization and sequential batch operation

Improving propionic acid production from a hemicellulosic hydrolysate of sorghum bagasse by means of cell immobilization and sequential batch operation

Metabolic profiling analysis of the vitamin B₁₂ producer Propionibacterium freudenreichii

Assessing rumen microbial composition and fibre attachment in dairy cows

Contact Info

Product

Resources

About

EPS production by Propionibacterium freudenreichii facilitates its immobilization for propionic acid production

Abstract: This study demonstrates the EPS-producing microbe to be easily immobilized on a solid matrix and to be used in a bioprocess. Such a system could be optimized for achieving high cell density in fermentations without the need for functionalization of the matrix.

Cited by 13 publications

References 36 publications

Improving propionic acid production from a hemicellulosic hydrolysate of sorghum bagasse by means of cell immobilization and sequential batch operation

Improving propionic acid production from a hemicellulosic hydrolysate of sorghum bagasse by means of cell immobilization and sequential batch operation

Metabolic profiling analysis of the vitamin B12 producer Propionibacterium freudenreichii

Assessing rumen microbial composition and fibre attachment in dairy cows

Contact Info

Product

Resources

About

Metabolic profiling analysis of the vitamin B₁₂ producer Propionibacterium freudenreichii