EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses

Collins, Carina A.; LaMontagne, Erica D; Anderson, Jeffrey C.; Ekanayake, Gayani; Clarke, Alexander S; Bond, Lauren N; Salamango, Daniel J.; Cornish, Peter V.; Peck, Scott C.; Heese, Antje

doi:10.1104/pp.19.01172

Cited by 28 publications

(22 citation statements)

References 91 publications

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“…The role of clathrin at the TGN has been a matter of much debate. Indirect evidence, especially obtained with the use of mutants in components associated with clathrin in the vesicle formation process at the TGN, argue for the involvement of CCVs either in vacuolar, or in exocytic, trafficking pathways (Park et al, 2013;Wang et al, 2014;Robinson and Pimpl, 2014;Feraru et al, 2010;Zwiewka et al, 2011;Sauer et al, 2013;Heinze et al, 2020;Collins et al, 2020). Our results from inducible downregulation of CHC expression support a function of clathrin in exocytosis of soluble and integral membrane protein cargo.…”

Section: An Involvement Of Clathrin In Exocytosissupporting

confidence: 53%

“…Additionally, evidence from loss-of-function mutants of an A. thaliana AP-3 adaptor complex, which in immunoprecipitation experiments binds clathrin, are too, indirectly indicative of a function of clathrin in the vacuolar pathway (Zwiewka et al, 2011;Feraru et al, 2010). In turn, loss of function mutants of TGN-localized monomeric clathrin adaptors MODIFIED TRANSPORT TO THE VACUOLE1 (MTV1) and EPSIN1 indicate clathrin functions in both vacuolar, and secretory, pathways (Sauer et al, 2013;Heinze et al, 2020;Collins et al, 2020). Finally, multivesicular bodies (MVBs), organelles acting as late endosomes in delivery of cargo to the vacuole, were observed to directly mature from the TGN compartment, and soluble vacuolar cargo to reach the vacuole following inhibition of clathrin function, arguing for a lack of necessity of clathrin-mediated vesicular transport for vacuolar delivery (Scheuring et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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The role of clathrin in exocytosis and the mutual regulation of endo- and exocytosis in plant cells

Adamowski

Matijević

Friml

2021

Preprint

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Within the plant endomembrane system, the vesicle coat protein clathrin localizes to the plasma membrane (PM) and the trans-Golgi Network/Early Endosome (TGN/EE). While the role of clathrin as a major component of endocytosis at the PM is well established, its function at TGN/EE, possibly in exocytosis or the vacuolar pathway, is a matter of debate. This shared function of clathrin also opens a question whether plant cells possess a homeostatic mechanisms that balance rates of opposite trafficking routes, such as endo- and exocytosis. Here we address these questions using lines inducibly silencing CLATHRIN HEAVY CHAIN (CHC). We find a relocation of exocytic soluble and integral membrane protein cargoes to the vacuole, supporting a function of clathrin in exocytosis. A comparison with lines overexpressing AUXILIN-LIKE1, where inhibition of CME precedes rerouting of secretory cargoes, does not support a homeostatic regulatory mechanism adjusting exocytosis to the rates of endocytosis. Complementary experiments reveal only minor and variably detectable reductions in the rates of CME in secretory mutants, also not indicative of a converse homeostatic mechanism adjusting rates of endocytosis to the rates of secretion.

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Section: An Involvement Of Clathrin In Exocytosissupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

The role of clathrin in exocytosis and the mutual regulation of endo- and exocytosis in plant cells

Adamowski

Matijević

Friml

2021

Preprint

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“…The assumption that Aleu-GFP sorting occurs through the Golgi apparatus thanks to the interaction with VSR1 is widely accepted. VSR1 interacts with Vti11 through the common interactors EPSIN1 [ 37 ] and VPS45 [ 17 , 38 ], while contacts with RMR1 are indirect.…”

Section: Resultsmentioning

confidence: 99%

Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER

et al. 2021

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Plants rely on both actin and microtubule cytoskeletons to fine-tune sorting and spatial targeting of membranes during cell growth and stress adaptation. Considerable advances have been made in recent years in the comprehension of the relationship between the trans-Golgi network/early endosome (TGN/EE) and cytoskeletons, but studies have mainly focused on the transport to and from the plasma membrane. We address here the relationship of the cytoskeleton with different endoplasmic reticulum (ER) export mechanisms toward vacuoles. These emergent features of the plant endomembrane traffic are explored with an in vivo approach, providing clues on the traffic regulation at different levels beyond known proteins’ functions and interactions. We show how traffic of vacuolar markers, characterized by different vacuolar sorting determinants, diverges at the export from the ER, clearly involving different components of the cytoskeleton.

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“…RNA isolation, cDNA synthesis, and RT-qPCR were performed as previously described (34). Unless stated otherwise, for each sample, three to five seedlings were elicited with the indicated concentration of flg22 peptide and placed at 22°C for the time indicated.…”

Section: Methodsmentioning

confidence: 99%

The phloem-resident OCTOPUS protein is a novel regulator of flg22-induced responses in Arabidopsis thaliana

Greenwood

King

Melena

et al. 2022

Preprint

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Phloem is a critical tissue that transports photosynthates and extracellular signals in vascular plants. Although a functional phloem is necessary for plant health, it is also an ideal environment for pathogens to access host nutrients to promote pathogenesis. Even though many vascular pathogens induce economically relevant crop damage, very little is known about the mechanism(s) by which phloem cells detect potential pathogens and signal to minimize damage. Our lab searched existing phosphoproteomic databases, mining for proteins that were phosphorylated in response to the defense-elicitor flagellin, or flg22, AND were expressed in vascular cells, and we identified Octopus (OPS). OPS is polarly associated with the plasma membrane (PM) of sieve element cells and promotes their differentiation from procambial precursor cells by inhibiting the function of BIN2 in brassinosteroid-related signaling. The observation that OPS is differentially phosphorylated in response to flg22 led us to the examine whether OPS may function in flg22-induced signaling using Arabidopsis T-DNA insertion mutants lacking a functional OPS. In wild-type (WT) seedlings, flg22 binds to the PM receptor flagellin sensing 2 (FLS2) to initiate three branches of a signaling cascade that culminates in increased expression of distinct marker genes. Ultimately these signaling pathways lead to the restriction of pathogen growth. Two independent alleles of ops were treated with 100 μM flg22 and marker genes from all three branches of FLS2 signaling exhibited higher expression than WT. We also found that in the absence of any flg22, ops mutants displayed increased flg22 signaling responses. Our results indicate that OPS may function as a negative regulator of flg22-induced signaling events and is one of very few phloem-resident proteins with a documented role in flg22 signaling. These results indicate that the phloem may be able to sense and response to the threat of bacterial pathogens in a unique way.

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EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses

Cited by 28 publications

References 91 publications

The role of clathrin in exocytosis and the mutual regulation of endo- and exocytosis in plant cells

The role of clathrin in exocytosis and the mutual regulation of endo- and exocytosis in plant cells

Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER

The phloem-resident OCTOPUS protein is a novel regulator of flg22-induced responses in Arabidopsis thaliana

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