Epstein-Barr virus <i>mir-bart1-5p</i> detection via nasopharyngeal brush sampling is effective for diagnosing nasopharyngeal carcinoma

Zheng, Xiaojing; Lu, Li Xia; Cui, Cui; Chen, Ming Yuan; Li, Xi Zhao; Jia, Wei Hua

doi:10.18632/oncotarget.6649

Cited by 36 publications

(38 citation statements)

References 22 publications

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“…EBV miR-BART1-5p and BART1-3p are dramatically increased in the N2-3 stages or advanced stages (III-IV) of NPC (Cai et al, 2015;Zheng et al, 2016). The levels of both miR-BART7 and miR-BART13 in NPC plasma are found to be present at distinct levels among patients with different stages of NPC, with elevated levels among patients with advanced disease.…”

Section: Ebv-encoded and Ebv-dysregulated Mirnas Are Potential Biomarmentioning

confidence: 95%

An update: Epstein-Barr virus and immune evasion via microRNA regulation

Zuo

Yue

et al. 2017

Virol. Sin.

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Epstein-Barr virus (EBV) is an oncogenic virus that ubiquitously establishes life-long persistence in humans.To ensure its survival and maintain its B cell transformation function, EBV has developed powerful strategies to evade host immune responses. Emerging evidence has shown that microRNAs (miRNAs) are powerful regulators of the maintenance of cellular homeostasis. In this review, we summarize current progress on how EBV utilizes miRNAs for immune evasion. EBV encodes miRNAs targeting both viral and host genes involved in the immune response. The miRNAs are found in two gene clusters, and recent studies have demonstrated that lack of these clusters increases the CD4 + and CD8 + T cell response of infected cells. These reports strongly indicate that EBV miRNAs are critical for immune evasion. In addition, EBV is able to dysregulate the expression of a variety of host miRNAs, which influence multiple immune-related molecules and signaling pathways. The transport via exosomes of EBV-regulated miRNAs and viral proteins contributes to the construction and modification of the inflammatory tumor microenvironment. During EBV immune evasion, viral proteins, immune cells, chemokines, pro-inflammatory cytokines, and pro-apoptosis molecules are involved. Our increasing knowledge of the role of miRNAs in immune evasion will improve the understanding of EBV persistence and help to develop new treatments for EBV-associated cancers and other diseases.

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Section: Ebv-encoded and Ebv-dysregulated Mirnas Are Potential Biomarmentioning

confidence: 95%

An update: Epstein-Barr virus and immune evasion via microRNA regulation

Zuo

Yue

et al. 2017

Virol. Sin.

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“…Nasopharyngeal brush sampling has been described in our previous studies . Briefly, it was conducted under the guidance of endoscopy by experienced specialists.…”

Section: Methodsmentioning

confidence: 99%

“…A final elution volume of 150 μL was obtained after the whole extraction procedure. As described in our previous study, total RNA from biopsy tissue and NP brush samples was extracted using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions. DNA and RNA concentration was quantified using a NanoDrop 1000 Spectrophotometer (NanoDrop Technologies).…”

Section: Methodsmentioning

confidence: 99%

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Detection of methylation status of Epstein‐Barr virus DNA C promoter in the diagnosis of nasopharyngeal carcinoma

Zheng

Wang

et al. 2020

Cancer Science

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The detection of Epstein-Barr virus (EBV) DNA load in nasopharyngeal (NP) brushing samples for diagnosis of nasopharyngeal carcinoma (NPC) has attracted great attention. Further improvements that eliminate the need for clinical settings will greatly extend its application. A total of 250 participants were recruited to obtain NP brushing samples. Brush sampling both with and without the guide of endoscopy was conducted in 38 NPC patients. EBV DNA load, EBV RNA transcript and EBV DNA C promoter methylation status were, respectively, evaluated. Typical latency II transcripts were observed in brushing samples from NPC patients but not controls. Unlike in tissues, multiple lytic gene transcripts were observed not only in NPC patients but also in controls. Apart from EBV RNA transcript, samples from NPC patients also showed higher levels of EBV DNA load and C promoter methylation degree than their controls. Qualitative analysis further showed that EBV DNA C promoter was methylated in all NPC patients but in only 18.4% of the control group. Combined analysis of EBV DNA methylated degree and EBV DNA load increased the sensitivity to 100% in the detection of NPC. Using qualitative methylated type as the criteria, up to 89.5% of samples collected via blind brushing showed consistent results with samples collected via endoscopy-guided brushing from NPC patients. Detection of the methylation status of EBV DNA C promoter in NP brushing samples shows great potential in diagnosing NPC and may provide an appealing alternative for the non-invasive detection and screening of NPC without the need for clinical settings. K E Y W O R D Sdiagnosis, Epstein-Barr virus DNA load, Epstein-Barr virus DNA methylation, nasopharyngeal brushing, nasopharyngeal carcinoma 1 | INTRODUC TI ON Nasopharyngeal carcinoma (NPC) is a highly invasive and metastatic cancer that is widely prevalent in southern China. Although the overall survival rate is approximately 90% in patients diagnosed with early clinical stage disease, unfortunately, most patients are diagnosed with advanced stage diseased at their first visit to the hospital, and the survival rate decreases to <50%. 1 Previous studies have S U PP O RTI N G I N FO R M ATI O NAdditional supporting information may be found online in the Supporting Information section.

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“…Besides plasma antibody and DNA, a circulating EBV miR-BART7 and miR-BART13 [58] were shown to serve as potentially new serological biomarkers for diagnosis and prediction of treatment efficacy of NPC. In a very recent study, Zheng et al demonstrated EBVencoded mir-bart1-5p detection via a less invasive nasopharyngeal brush sampling can diagnose early-stage NPC with 93.5% sensitivity and 100% specificity [59]. However, all these miRNA detections need large patient cohorts across multiple centers for confirmation.…”

Section: Ebv-based Npc Screening and Diagnosismentioning

confidence: 99%

The other side of the coin: Leveraging Epstein–Barr virus in research and therapy

Tan

et al. 2016

Oral Oncology

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Epstein-Barr virus is (EBV) a ubiquitous virus prevalent in 90% of the human population. Transmitted through infected saliva, EBV is the causative agent of infectious mononucleosis (IM) and is further implicated in malignancies of lymphoid and epithelial origins. In the past few decades, research efforts primarily focused on dissecting the mechanism of EBV-induced oncogenesis. Here, we present an alternate facet of the oncovirus EBV, on its applications in research and therapy. Finally, discussions on the prospective utilization of EBV in nasopharyngeal carcinoma (NPC) diagnosis and therapy will also be presented.

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Epstein-Barr virus mir-bart1-5p detection via nasopharyngeal brush sampling is effective for diagnosing nasopharyngeal carcinoma

Cited by 36 publications

References 22 publications

An update: Epstein-Barr virus and immune evasion via microRNA regulation

An update: Epstein-Barr virus and immune evasion via microRNA regulation

Detection of methylation status of Epstein‐Barr virus DNA C promoter in the diagnosis of nasopharyngeal carcinoma

The other side of the coin: Leveraging Epstein–Barr virus in research and therapy

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