Stormwater runoff from highways is a source of pollution to surface water bodies and groundwater. Excess loadings of phosphorus in stormwater discharged to surface water bodies can result in eutrophication. Treatment of stormwater for phosphorus is necessary in order to sustain ecological and economical benefits related to aquatic resources. If phosphorus is removed, the water can be sustained for other uses, such as irrigation and industrial applications. The data presented in this paper is used to evaluate the treatment performance of a roadside biosorption activated media system with regards to the removal of total phosphorus and soluble reactive phosphorus from highway runoff. The evaluation also compares removal efficiencies and effluent concentrations using biosorption activated media (BAM) to that with sandy soil commonly found in the Florida area. The results presented in this paper indicate that BAM bio-filtration systems are a feasible treatment method for removing phosphorus from highway runoff. A discussion concerning the additional treatment and reuse of water by harvesting, as part of a Bio-filtration & Harvesting Swale System, is also presented in the paper.