Equine autologous platelet concentrates: A comparative study between different available systems

Abstract

Haematological and biochemical characteristics varied markedly among 5 techniques used in the field to produce APCs in horses. These discrepancies could have an impact on clinical outcomes, and further studies are needed to determine their influence on the quality of tissue regeneration. Clinicians should not rely on the manufacturers' data relating to human patients to select the most appropriate method for horses.

Effects of Autologous Conditioned Plasma® (ACP) on the healing of surgically induced core lesions in equine superficial digital flexor tendon

“…Nevertheless, as conventional plasma and ACP samples were treated identically, there is no doubt about the highly significant increase in PDGF-BB concentration in ACP samples. Even though there was a significant difference in the PDGF-BB concentration, these results were significantly lower when compared to previously reported values (Hessel et al 2014, Kissich et al 2012. Interestingly, a recent study suggests that ACP is apparently not as efficient concentrating PDGF-BB and TGF-b as it was previously reported (Hessel et al 2014), this might suggest that the growth factor differences found in these 3 studies could have been caused by a low repeatability of the ACP kit when using horse blood.…”

“…Even though there was a significant difference in the PDGF-BB concentration, these results were significantly lower when compared to previously reported values (Hessel et al 2014, Kissich et al 2012. Interestingly, a recent study suggests that ACP is apparently not as efficient concentrating PDGF-BB and TGF-b as it was previously reported (Hessel et al 2014), this might suggest that the growth factor differences found in these 3 studies could have been caused by a low repeatability of the ACP kit when using horse blood. This is especially true when comparing the values reported by Hessel and others (2014) and Kissich and other (2012), where ACD-A and a very similar processing protocol were used.…”

“…Interestingly, the PDGF-BB concentrations achieved in this study were lower and the TGF-b were higher (but not significantly higher than conventional plasma) than the reported values using anticoagulant citrate dextrose solution A (ACD-A) (Hessel et al 2014, Kissich et al 2012. Several studies have !…”

“…This hypothesis is also supported by the fact that the PDGF-BB values were significantly increased in the ACP samples, which can only be explained by concentration of the platelets. On the other hand, recent studies suggest that the ACP kit might not be as efficient concentrating platelets as it was previously reported (Hessel et al 2014). This might be an easier explanation for the low platelet counts, but would not explained the increase in PDGF-BB concentration.…”

Qualitative comparison between equine bone marrow- and blood-derived autologous conditioned serum

“…Anschließend wurde der Überstand (KM-ACS) in eine 12 ml Polypropylen Spritze aufgezogen. (Giraldo et al 2013, Hessel et al 2015, Ionita et al 2016. Dies bestätigt, dass es sich bei Orthobiologika nicht um standardisierte Therapeutika handelt, was die Etablierung von Behandlungsprotokollen erschwert und somit keine zuverlässige individuelle Aussage über die enthaltenen Inhaltsstoffe bzw.…”