Equine sperm–oocyte interaction: results after intraoviductal and intrauterine inseminations of recipients for oocyte transfer

Carnevale, E.M.; Maclellan, L.J.; Silva, M.A. Coutinho da; Checura, Celina M.; Scoggin, C.F.; Squires, E.L.

doi:10.1016/s0378-4320(01)00167-1

Cited by 20 publications

(10 citation statements)

References 11 publications

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“…Conventional in vitro fertilization has not been repeatably successful, although recent findings on the induction of hyperactivated motility to achieve fertilization may provide a mechanism for this in the future (McPartlin et al, 2009). Because of this, methods for achieving in vivo fertilization after transfer of oocytes to the oviducts of inseminated recipient mares have been developed, and these have been used to explore the physiology of fertilization in this species (Carnevale and Ginther, 1995; Carnevale et al, 2001; Maclellan et al, 2002).…”

Section: Developmental Competencementioning

confidence: 99%

The equine oocyte: Factors affecting meiotic and developmental competence

Hinrichs

2010

Molecular Reproduction Devel

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SUMMARYThere is currently much interest in assisted reproduction techniques in the horse, however, many aspects of oocyte maturation, fertilization, and embryo development in the horse differ from those in other species. Because of the close attachment of the equine oocyte to the follicle wall, scraping of the follicle is the most effective method for oocyte recovery. A notable feature of equine oocytes is that those with expanded cumuli (Ex oocytes), which originate from atretic follicles, have higher meiotic competence (ability to mature to metaphase II in vitro) than do oocytes with compact cumuli (Cp oocytes). Cp oocytes originate in viable follicles but are largely juvenile. Recovery and culture of equine oocytes immediately after slaughter yields a higher maturation rate than that obtained from oocytes after ovary storage; this is related to damage to chromatin in Cp oocytes during storage. In contrast, developmental competence (rate of blastocyst development in vitro) is higher in oocytes recovered from the ovary after a delay. The optimum duration of maturation varies based on cumulus morphology and time of recovery from the ovary, but there is no difference in developmental competence between Ex and Cp oocytes. Because standard in vitro fertilization is not repeatable in the horse, oocyte transfer (surgical transfer of oocytes to the oviducts of inseminated mares) has been developed to allow fertilization of isolated oocytes. Fertilization in vitro may be achieved using intracytoplasmic sperm injection; culture of injected oocytes in a medium with high glucose can yield over 30% blastocyst development.Mol. Reprod. Dev. 77: 651-661,

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Section: Developmental Competencementioning

confidence: 99%

The equine oocyte: Factors affecting meiotic and developmental competence

Hinrichs

2010

Molecular Reproduction Devel

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“…We used a deep‐horn technique to inseminate the mares that may have accounted for the maintenance of fertility using the lower dose. In this regard, the semen deposition at the tip of uterine horn permits a greater fraction of viable spermatozoa to colonise within the oviduct and hence fewer spermatozoa is needed to achieve the same fertility than with conventional AI at the uterine body (Carnevale et al ., ). In addition, the use of the present proposed freezing technique using 150–300 million progressively motile spermatozoa per insemination enabled a higher fertility (> 75% of pregnant mares after a single insemination) if compared to pregnancy rate obtained in other studies (30–60%; Müller, ; Samper, ; Barbacini et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Impact of using a fast-freezing technique and different thawing protocols on viability and fertility of frozen equine spermatozoa

2013

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The effects of freezing technique and thawing protocol on thawed semen viability and fertility were studied. Ejaculates from 5 stallions (n = 25) were frozen by conventional or a fast-freezing technique. Frozen semen was thawed by two thawing protocols (37 °C 30 s(-1) or 75 °C 7 s(-1) ). Thawed semen was evaluated by progressive motility, vigour, morphology and plasma membrane integrity. Mares (n = 25) were inseminated with 300 (n = 11) or 150 (n = 14) million spermatozoa. A greater (P < 0.05) vigour and progressively motile spermatozoa were detected, respectively, at thawing and after 20 min post-thawing in the fast-freezing technique than in the conventional one. Plasma membrane integrity was also greater (P < 0.05) in semen frozen with the fast-freezing technique. Semen viability was not affected by thawing protocol. Pregnancy rate using the fast-freezing technique was 76% (19/25), and did not differ (P > 0.05) between insemination doses. We concluded that the 150 million progressively motile spermatozoa per dose using a deep-horn insemination maximises the use of equine semen. The fast-freezing technique, as compared to the conventional one, efficiently preserves the viability and fertilising capacity of spermatozoa, indicating a new method to improve the fertility of frozen equine semen.

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“…Carnevale and colleagues [58] achieved conception rates of 67% (12 of 18 mares) after GIFT of an oocyte with 2 to 5 Â 10 5 fresh spermatozoa. The success of GIFT demonstrates that complete capacitation can occur in the oviduct in the absence of a uterine phase of sperm transport.…”

Section: Oviductal Inseminationmentioning

confidence: 98%

Advanced Insemination Techniques in Mares

Morris

2006

Veterinary Clinics of North America: Equine Practice

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Equine sperm–oocyte interaction: results after intraoviductal and intrauterine inseminations of recipients for oocyte transfer

Cited by 20 publications

References 11 publications

The equine oocyte: Factors affecting meiotic and developmental competence

The equine oocyte: Factors affecting meiotic and developmental competence

Impact of using a fast-freezing technique and different thawing protocols on viability and fertility of frozen equine spermatozoa

Advanced Insemination Techniques in Mares

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