Equipment and growth inhibition of thermophilic bacteria

Sonnleitner, Bernhard; Cometta, S.; Fiechter, Armin

doi:10.1002/bit.260241123

Cited by 17 publications

(4 citation statements)

References 3 publications

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“…A significant change in behavior was observed when ES4 was incubated in a stainless steel vessel compared with the same vessel subsequently lined with glass. Although there were no apparent effects of the steel vessel in previous high-pressure experiments with the deep-sea methanogen Methanococcus jannaschii (20), stainless steel inhibits the growth of some thermophilic aerobes (28), and corrosion of steel vessels by H2, sulfide, and seawater is common even at moderate temperatures (31). Potentially inhibitory metal ions that can leach from corroding steel include Fe, Co, Ni, Mn, and Cr (28).…”

Section: Discussionmentioning

confidence: 99%

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Effects of Hyperbaric Pressure on a Deep-Sea Archaebacterium in Stainless Steel and Glass-Lined Vessels

Nelson

Clark

1991

Appl Environ Microbiol

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The effects of hyperbaric helium pressures on the growth and metabolism of the deep-sea isolate ES4 were investigated. In a stainless steel reactor, cell growth was completely inhibited but metabolic gas production was observed. From 85 to 100°C, CO2 production proceeded two to three times faster at 500 atm (1 atm = 101.29 kPa) than at 8 atm. At 105°C, no CO2 was produced until the pressure was increased to 500 atm. Hydrogen and H2S were also produced biotically but were not quantifiable at pressures above 8 atm because of the high concentration of helium. In a glass-lined vessel, growth occurred but the growth rate was not accelerated by pressure. In most cases at temperatures below 100°C, the growth rate was lower at elevated pressures; at 100°C, the growth rates at 8, 250, and 500 atm were nearly identical. Unlike in the stainless steel vessel, CO2 production was exponential during growth and continued for only a short time after growth. In addition, relatively little H2 was produced in the glass-lined vessel, and there was no growth or gas production at 105°C at any pressure. The behavior of ES4 as a function of temperature and pressure was thus very sensitive to the experimental conditions.

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Section: Discussionmentioning

confidence: 99%

“…Passivation with nitric acid has been shown to reduce the growth inhibition by stainless steel of some aerobic thermophiles (28). However, with ES4 the inhibitory effect was not reversed by pretreating the steel vessel with HNO3.…”

Section: Discussionmentioning

confidence: 99%

Effects of Hyperbaric Pressure on a Deep-Sea Archaebacterium in Stainless Steel and Glass-Lined Vessels

Nelson

Clark

1991

Appl Environ Microbiol

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“…in a 20 1 'Intensor b-20'fermenter (Giovanola Freres). Before each fermentation run the fermenter pot was treated with 25% (v/v) nitric acid for 16 h in order to passivate its steel surface (Sonnleitner et al, 1982). The cells were harvested by centrifugation.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of an extremely thermostable D-xylose isomerase from Thermus aquaticus HB 8

Lehmacher

Bisswanger

1990

Journal of General Microbiology

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The extremely &hemophilic organism Thermus quaticus possesses high activities of enzymes catalysing the degradation of xyians and metabolizing ~x y l o s e via the pntose phosphate pathway. The D-xylsse isomerase (Dxylsse ketoll-isomerase, EC 5 o 3 o I . 51, an important enzyme of this process, is efficiently induced by its substrate Dxylose, and, 40 a lesser extent, by related pentoses and some derivatives of 1p-xylose. The ~-xyPose isomerase from T. qrsatkus has been g u~e d by anion-exchange chrmmtography, chromatography on ID-xylkose agarose and gcE filtration. A single band migrating according to an Mr of 5 0 0 was obtained by SDS-PAGE. An M,. oh 194800 for the native enzyme, determined by gel filtration and ultracentrifugation in a glycerol gradient, suggested that the Dxylsw isomerase is a homomeric tetramer. Amhenius plots of the enzyme activity of the D-xylose isomerase weref linear up to a temperature of $5 OC. At 90 "C the enzyme was inactivated in the absence of divalent c;~PPoRs, with a haIf4ife of 4 d, while in the presence of Mn2+ or Co2+ it remained fully active for at least 1 mo~ith. The enzyme $ilia2 an isoelectric point at 4.4 and showed a broad optimum in the pH range from 5-5 to 8-5. NQ significant difkepemces in the pH and temperature behaviour could be observed when ~x y l o s e was compared with D -~~U C Q W as substrate. Digearen& methods of immobilization of the enzyme to solid supports as well as inclusion into nyhm beads w m studied. Attachment of the enzyme to epoxy-activated agarose and its m-aggregation with bovine serum albumin gave immobilized preparations with the same stability as free enzyme supplemente i t h hh2+ QP cQ*+e

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“…Conventional steels and plastics undergo deterioration under these extreme conditions [63] and alternative materials such as advanced ceramics will be required for any future industrial applications. The economics of metal processing under such conditions will be dictated by the value of the material and the enhancement or uniqueness of microbial processing compared to chemical or physical techniques.…”

Section: Current Nbs Activities In Inorganic Materials Bioprocessingmentioning

confidence: 99%

Inorganic Materials Biotechnology: A New Industrial Measurement Challenge

Olson¹,

Brinckman²

1986

J. RES. NAT. BUR. STAN.

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Biotechnological processing of inorganic, heavy elements has only begun to emerge as we start to understand microbial strategies and mechanisms of heavy element transformations. Chemical speciation of key, diagnostic intermediates and products of bioprocessing in gas, liquid. and cellular phases, and on surfaces, is required to understand and optimize important reactions. Recent discoveries of microorganisms in metalenriched thermal environments, and further investigations into production of exocellular metal transforming metabolites, offer exciting prospects for development of new technologies for strategic and precious materials recovery and processing.

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Equipment and growth inhibition of thermophilic bacteria

Cited by 17 publications

References 3 publications

Effects of Hyperbaric Pressure on a Deep-Sea Archaebacterium in Stainless Steel and Glass-Lined Vessels

Effects of Hyperbaric Pressure on a Deep-Sea Archaebacterium in Stainless Steel and Glass-Lined Vessels

Isolation and characterization of an extremely thermostable D-xylose isomerase from Thermus aquaticus HB 8

Inorganic Materials Biotechnology: A New Industrial Measurement Challenge

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