As older adults face increasing mobility challenges due to diminished driving ability, they encounter heightened risks of social exclusion, impacting their mental, social, and physical well-being. This study investigates spatial gaps in the availability of sustainable transportation options—including public transit, paratransit, and specialized senior services—and examines their alignment with the distribution of older adults segmented by age group (65–74, 75–84, and 85+). Using Florida as a case study, Geographic Information System (GIS) was employed to conduct a geospatial analysis, identifying statistically significant clusters of low transportation availability alongside high concentrations of older adults. The primary contribution of this research lies in its innovative methodology, which integrates precise transportation service boundaries with age-segmented demographic data, offering a nuanced assessment of transportation equity as a crucial aspect of sustainability. Findings provide a comprehensive framework for policymakers, enabling targeted resource allocation and planning that enhance mobility, accessibility, and quality of life for older adults. This study contributes to advancing sustainable development goals by addressing transportation disparities, supporting equitable, age-sensitive transportation solutions, and informing broader discussions on sustainable urban planning.