The aims of this study were to determine the concentration of particulate matter, analyze the percentage share of four particulate matter subfractions (PM1, PM2.5, PM4, PM10) in TSP (total mass of particulate matter (PM)) in a typical Polish sports hall at different day periods during heating and non-heating seasons, and compare the average daily doses of respirable dust (PM4) for three groups of the sports hall users (pupils, teachers, and athletes). Gravimetric measurements of PM4 and TSP concentrations and optical measurements of the concentrations of five PM fractions (PM1, PM2.5, PM4, PM10, PM100) were conducted for 8 hours a day, simultaneously inside and outside the hall, for 20 days each in summer and winter. During training, PM mass was concentrated mainly in coarse particles (PM2.5–100) (summer—55%, winter—35%). Without activity, the main part of PM mass was from fine particles (PM2.5, summer—59%, winter—75%). In summer, PM inside the hall originated mainly from internal sources. In winter, the fine PM concentration was affected by outdoor sources. The daily doses of PM4 for different groups of sports hall users indicate that the health exposure of sports practitioners to PM may be greater than for non-practitioners staying in the same conditions.