A significant number of wind bracings in existing railway transom top bridges are numerically assessed deficient against the assessment nosing load recommended by the AS5100, where in almost all cases, there is no observed evidence of wind bracings being overloaded. This paper estimates the nosing load applied by various trains to a couple of random spans of an existing railway transom top bridge. Firstly, field testing of this bridge is conducted and the measured stresses at the mid-center of girders and wind bracings are collected during various normal train operations to validate the developed Finite Element (FE) models of this bridge. Then, the nosing loads due to different trains are estimated using the validated FE model through a two-staged validation approach, including automatic FE stress intensity optimization and rigorous manual FE model sensitivity analysis while transoms in various conditions are also incorporated in the FE model. Results demonstrate that the nosing load is significantly less than the required load in the AS5100 with magnitudes ranging between 8.6% to 9.4% of the maximum vertical axle load of the passed trains; suggesting that the AS5100 assessment nosing load should be revised to avoid unnecessary expensive upgrades of numerically assessed deficient wind bracings.