Background With the expanding implementation of minimally invasive surgery, the operating team is confronted with challenges in the field of ergonomics. Visual feedback is derived from a monitor placed outside the operating field. This crossover trial was conducted to evaluate and compare neck posture in relation to monitor position in a dedicated minimally invasive surgery (MIS) suite and a conventional operating room. Methods Assessment of the neck was conducted for 16 surgeons, assisting surgeons, and scrub nurses performing a laparoscopic cholecystectomy in both types of operating room. Flexion and rotation of the cervical spine were measured intraoperatively using a video analysis system. A two-question visual analog scale (VAS) questionnaire was used to evaluate posture in relation to the monitor position. Results Neck rotation was significantly reduced in the MIS suite for the surgeon (p = 0.018) and the assisting surgeon (p \ 0.001). Neck flexion was significantly improved in the MIS suite for the surgeon (p \ 0.001) and the scrub nurse (p = 0.018). On the questionnaire, the operating room team scored their posture significantly higher in the MIS suite and also indicated fewer musculoskeletal complaints.Conclusions The ergonomic quality of the neck posture is significantly improved in the MIS suite for the entire operating room team.Keywords Ergonomics Á Laparoscopic cholecystectomy Á Minimally invasive surgery Á Neck Á Operating room Á Posture Minimally invasive surgery (MIS) plays a major part in modern abdominal surgery, urology, and gynecology and has become the treatment of choice for a still growing number of procedures. Most of the advantages with MIS are patient related. Less blood loss, less postoperative pain, shorter hospital admissions, quicker reintroduction into society, and a superior cosmetic result are some wellestablished MIS advantages [1][2][3][4].On the other hand, MIS confronts the surgeon and his or her team with some challenging aspects, primarily in the area of ergonomics and efficiency [5,6]. The necessity of additional equipment-including electrocautery and insufflation devices, monitors, video equipment, wiring, and tubing, usually stored outside the operating room on large heavy trolleys-has compromised operating room efficiency and prolonged turnover times [7].During the procedure, the surgeon must work with long instruments that move invertedly inside the abdomen and with a certain scaling effect, also known as the fulcrum effect [8]. The entire operating team derives the visual feedback of their actions from a monitor positioned on top of a laparoscopic trolley that stands outside the operative field and away from the patient. Due to this positioning, the