ERIC-PCR Genotyping of SomeCampylobacter jejuniIsolates of Chicken and Human Origin in Egypt

Abstract

The public health importance of the genus Campylobacter is attributed to several species causing diarrhea in consumers. Poultry and their meat are considered the most important sources of human campylobacteriosis. In this study, 287 samples from chicken (131 cloacal swabs, 39 chicken skin, 78 chicken meat, and 39 cecal parts) obtained from retail outlets as well as 246 stool swabs from gastroenteritis patients were examined. A representative number of the biochemically identified Campylobacter jejuni isolates were identified by real-time PCR, confirming the identification of the isolates as C. jejuni. Genotyping of the examined isolates (n = 31) by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) revealed a high discriminatory index of ERIC-PCR (D = 0.948), dividing C. jejuni isolates of chicken and human origins into 18 profiles and four clusters. The 18 profiles obtained indicated the heterogeneity of C. jejuni. Dendrogram analysis showed that four clusters were generated; all human isolates fell into clusters I and III. These observations further support the existence of a genetic relationship between human and poultry isolates examined in the present study. In conclusion, the results obtained support the speculation that poultry and poultry meat have an important role as sources of infection in the acquisition of Campylobacter infection in humans.

Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)

“…This result is in akin with the reports of [ 39 – 41 ], who have also reported high diversity of Campylobacter species obtained from porcine, avian, turkey, and broiler, respectively. The UPGMA dendrogram clustering image of the 36 C. jejuni isolates genotyped grouped the isolates into four clusters and this finding is in line with the report of [ 42 , 43 ]. Result obtained from C. jejuni fingerprinting is contrary to the report of [ 44 ], who in their report revealed that 15 C. jejuni genotyped were grouped into 10 genotypes with 3 clusters.…”

Molecular Characterization of Escherichia coli O157:H7 and non-O157 Shiga Toxin Producing E. coli from Retail Meat and Humans

“…Molecular typing is useful to determine the genetic relationship of pathogens isolated from different sources. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC PCR) is a simple, cost effective tool with high discriminatory power for genotyping of different bacterial pathogens [11][12][13]. In order to investigate the occurrence of STEC in Zagazig City, Egypt, minced beef and chicken meat purchased from food outlets and human samples from outpatient clinics at Sharkia Governorate were examined.…”

“…ERIC-PCR has been used previously for genotyping of different bacteria including E. coli isolates of different origins in order to determine the genetic relationship between the isolates [11,12,65]. …”

One health approach of campylobacteriosis in Egypt: An emerging zoonotic disease

“…Poultry is recognized as a primary source for Campylobacter spp. transmission to humans as strain of C. jejuni can survive in chicken droppings for up to 6 days after excretion [13]. Comprehensive studies conducted in different Egyptian governorates proved that chickens reared in farms or houses is the main source of Campylobacter infections for contact persons [14][15][16][17].…”