Erken Osteojenik Farklılaşma (In Vitro) Süresince Wistar Sıçanlarda (Rattus novergicus) Gingival Medicinal Signaling Hücrelere Post Trombositten Zengin Fibrin Uygulamasının Agrekan Ekspresyonuna Etkisi

Nugraha, Alexander Patera; Narmada, Ida Bagus; Ernawati, Diah Savitri; Dinaryanti, Aristika; Hendrianto, Eryk; Ihsan, Igo Syaiful; Riawan, Wibi; Rantam, Fedik Abdul

doi:10.9775/kvfd.2018.21174

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…The increase in collagen gene expression induced by PRF was reported in several studies [27,69,75,78,79], typically around the 14th to the 21st day of exposure. Zhao et al [88] observed slight upregulation by PRF in a dose-dependent manner after 14 or 21 days of culture (p < 0.01).…”

Section: The Molecular Effects Of Prf On Differentiation and Minerali...mentioning

confidence: 63%

“…The study by Nugraha et al [67] investigated the effects of PRF on the osteogenic differentiation of GSPCs, identifying a role of the Cbfa1/Sox9 expression ratio in this process, as it positively correlated with the osteogenic markers and the mineralization of GSPCs. Later, another study by this group [69] showed that PRF also increased the expression of aggrecan, a chondrogenic differentiation marker that has a significant role in the early stage of osteogenic differentiation of gingival stem cells, which may contribute to accelerating bone remodeling with an increased expression of alkaline phosphatase and osteocalcin [67,68].…”

Section: Cell-type Related Effects Of Prfmentioning

confidence: 99%

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The Effects of Platelet-Rich Fibrin in the Behavior of Mineralizing Cells Related to Bone Tissue Regeneration—A Scoping Review of In Vitro Evidence

de Lima Barbosa,

Stellet Lourenço,

de Azevedo dos Santos

et al. 2023

JFB

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Platelet-rich fibrin (PRF) is a second-generation blood concentrate that serves as an autologous approach for both soft and hard tissue regeneration. It provides a scaffold for cell interaction and promotes the local release of growth factors. PRF has been investigated as an alternative to bone tissue therapy, with the potential to expedite wound healing and bone regeneration, though the mechanisms involved are not yet fully understood. This review aims to explore the in vitro evidence of PRF’s effects on the behavior of mineralizing cells related to bone tissue regeneration. A systematic electronic search was conducted up to August 2023, utilizing three databases: PubMed, Web of Science, and Scopus. A total of 76 studies were selected, which presented in vitro evidence of PRF’s usefulness, either alone or in conjunction with other biomaterials, for bone tissue treatment. PRF membranes’ influence on the proliferation, differentiation, and mineralization of bone cells is linked to the constant release of growth factors, resulting in changes in crucial markers of bone cell metabolism and behavior. This further reinforces their therapeutic potential in wound healing and bone regeneration. While there are some notable differences among the studies, the overall results suggest a positive effect of PRF on cell proliferation, differentiation, mineralization, and a reduction in inflammation. This points to its therapeutic potential in the field of regenerative medicine. Collectively, these findings may help enhance our understanding of how PRF impacts basic physiological processes in bone and mineralized tissue.

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Section: The Molecular Effects Of Prf On Differentiation and Minerali...mentioning

confidence: 63%

Section: Cell-type Related Effects Of Prfmentioning

confidence: 99%

The Effects of Platelet-Rich Fibrin in the Behavior of Mineralizing Cells Related to Bone Tissue Regeneration—A Scoping Review of In Vitro Evidence

de Lima Barbosa,

Stellet Lourenço,

de Azevedo dos Santos

et al. 2023

JFB

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show abstract

“…This shows that the collagen in gourami fish scales, when used as a scaffold, can bind and store growth factors (Zhang et al, 2011). Growth factors are known to stimulate and increase the expression of SOX9 from day 7 of osteogenic differentiation, which is thus useful for accelerating bone remodeling and cartilage formation (Nugraha et al, 2019).…”

Section: Groupsmentioning

confidence: 98%

Expression of Osterix and SOX9 after Administration of Gourami Fish Scale Collagen in Wistar Rats

Setiawan¹,

Prahasanti²,

Tionardus³

et al. 2023

NLSC

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The current goal of periodontal treatments is to regenerate periodontal tissue by adding bone grafts. Various materials are being explored as bone substitutes, including type 1 collagen. Gourami fish scales are known to be an alternative source of type 1 collagen and have potential as a substitute material for bone grafts. The objective of this study was to determine whether there is an increase in the expression of Osterix (OSX) and SOX9 after the administration of gourami (Osphronemus goramy) fish scale collagen in extracted socket Wistar rats (Rattus norvegicus). The research was conducted on 32 male Wistar rats that were classified into four groups: control groups on day 7 (K7) and day 14 (K14); and treatment groups on day 7 (P7) and day 14 (P14). The expression of OSX and SOX9 was visualized by immunohistochemical staining of bone tissue preparations by anti-OSX and SOX9 monoclonal antibodies. The ANOVA results for OSX expression had a significant difference in OSX expression between that in the control and treatment groups on day 7 and day 14. The ANOVA results for SOX9 expression on day 7 had a significant difference in SOX9 expression between that in the control and treatment groups. ANOVA results for SOX9 expression on day 14 had no significant difference in the expression of SOX9 between the treatment and control groups. The conclusion of this study was administration of Gourami scale collagen can increase the expression of OSX and SOX9 in socket’s Wistar rats. Keywords: Bone grafts, Type 1 collagen, Gourami fish scales, Osterix, SOX9

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The distribution pattern and growth factor level in platelet-rich fibrin incorporated skin-derived mesenchymal stem cells: An in vitro study

et al. 2020

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Background and Aim: A skin wound in an animal must be cared for to prevent further health issues. Platelet-rich fibrin (PRF) and skin-derived mesenchymal stem cells (SMSCs) have been reported to have potential in increasing the rate of wound healing. This study aimed to analyze the distribution patterns and levels of platelet-derived growth factor (PDGF), insulin-like growth factor (IGF), vascular endothelial growth factor (VEGF), and transforming growth factor-β (TGF-β) in PRF incorporated with SMSCs. Materials and Methods: This study employed a true experiment (in vitro) design with post-test only performed in the control group alone. PRF and SMSCs were extracted from the blood and skin of 16 rabbits. SMSCs were characterized using immunocytochemistry to examine clusters of differentiation for 45, 73, 90, and 105. PRF was incorporated into the SMSCs and then divided into four groups (N=32/n=8): Group A (PRF only), Group B (PRF+SMSCs, incubated for 1 day), Group C (PRF+SMSCs, incubated for 3 days), and Group D (PRF+SMSCs, incubated for 5 days). Scanning electron microscopy was used to examine the distribution pattern of SMSCs between groups. The supernatant serum (Group A) and supernatant medium culture (Group D) were collected for the measurement of PDGF, IGF, VEGF, and TGF-β using an enzyme-linked immunosorbent assay sandwich kit. An unpaired t-test was conducted to analyze the differences between Groups A and D (p<0.01). Results: Group D had the most morphologically visible SMSCs attached to the PRF, with elongated and pseudopodia cells. There was a significant difference between the levels of growth factor in Groups A and D (p=0.0001; p<0.01). Conclusion: SMSCs were able to adhere to and distribute evenly on the surface of PRF after 5 days of incubation. The PRF incorporated SMSCs contained high levels of PDGF, IGF, VEGF, and TGF- β, which may prove to have potential in enhancing wound healing.

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Erken Osteojenik Farklılaşma (In Vitro) Süresince Wistar Sıçanlarda (Rattus novergicus) Gingival Medicinal Signaling Hücrelere Post Trombositten Zengin Fibrin Uygulamasının Agrekan Ekspresyonuna Etkisi

Cited by 6 publications

References 13 publications

The Effects of Platelet-Rich Fibrin in the Behavior of Mineralizing Cells Related to Bone Tissue Regeneration—A Scoping Review of In Vitro Evidence

The Effects of Platelet-Rich Fibrin in the Behavior of Mineralizing Cells Related to Bone Tissue Regeneration—A Scoping Review of In Vitro Evidence

Expression of Osterix and SOX9 after Administration of Gourami Fish Scale Collagen in Wistar Rats

The distribution pattern and growth factor level in platelet-rich fibrin incorporated skin-derived mesenchymal stem cells: An in vitro study

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