EROD activity in liver and gills of polar cod (Boreogadus saida) exposed to waterborne and dietary crude oil

Nahrgang, Jasmine; Jönsson, Martina; Camus, Lionel

doi:10.1016/j.marenvres.2010.02.003

Cited by 20 publications

(6 citation statements)

References 18 publications

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“…In the North Sea, it is expected that demersal fish experience water-borne exposures in addition to oral intake while bottom-feeding. The smaller 2-and 3-ring PAHs like those in PW treatment are more soluble in water, while the heavier PAHs in Oil and PAH are more likely to be adsorbed to organic material and taken in as food [43]. From our studies, we saw levels of DNA adducts that are comparable with what has been observed for wild fish, despite only using oral doses in the experimental design.…”

Section: Dna Adducts and Health Effectssupporting

confidence: 73%

DNA damage and health effects in juvenile haddock (Melanogrammus aeglefinus) exposed to PAHs associated with oil-polluted sediment or produced water

et al. 2020

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The research objective was to study the presence of DNA damages in haddock exposed to petrogenic or pyrogenic polyaromatic hydrocarbons (PAHs) from different sources: 1) extracts of oil produced water (PW), dominated by 2-ring PAHs; 2) distillation fractions of crude oil (representing oil-based drilling mud), dominated by 3-ring PAHs; 3) heavy pyrogenic PAHs, mixture of 4/5/6-ring PAHs. The biological effect of the different PAH sources was studied by feeding juvenile haddock with low doses of PAHs (0.3-0.7 mg PAH/kg fish/ day) for two months, followed by a two-months recovery. In addition to the oral exposure, a group of fish was exposed to 12 single compounds of PAHs (4/5/6-ring) via intraperitoneal injection. The main endpoint was the analysis of hepatic and intestinal DNA adducts. In addition, PAH burden in liver, bile metabolites, gene and protein expression of CYP1A, GST activity, lipid peroxidation, skeletal deformities and histopathology of livers were evaluated. Juvenile haddock responded quickly to both intraperitoneal injection and oral exposure of 4/ 5/6-ring PAHs. High levels of DNA adducts were detected in livers three days after the dose of the single compound exposure. Fish had also high levels of DNA adducts in liver after being fed with extracts dominated by 2-ring PAHs (a PW exposure scenario) and 3-ring PAHs (simulating an oil exposure scenario). Elevated levels of DNA adducts were observed in the liver of all exposed groups after the 2 months of recovery. High levels of DNA adduct were found also in the intestines of individuals exposed to oil or heavy PAHs, but not in the PW or control groups. This suggests that the intestinal barrier is very important for detoxification of orally exposures of PAHs.

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Section: Dna Adducts and Health Effectssupporting

confidence: 73%

DNA damage and health effects in juvenile haddock (Melanogrammus aeglefinus) exposed to PAHs associated with oil-polluted sediment or produced water

et al. 2020

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“…There are numerous previous studies on EROD activity in liver but few studies have been conducted in gills (Mdegela et al, 2006;Ortiz-Delgado et al, 2008;Nahrgang et al, 2010) and only a few of them in relation with heavy metals (Mondon et al, 2001;Jönsson et al, 2004).…”

Section: Gill Erod Activitymentioning

confidence: 99%

EROD activity and cytochrome P4501A induction in liver and gills of Senegal sole Solea senegalensis from a polluted Huelva Estuary (SW Spain)

Oliva

Gravato

Guilhermino

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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EROD activity and induction cytochrome P4501A in liver and gills of Senegal sole, Solea senegalensis, from a heavy metal and PAH polluted estuary, was studied. Liver and gill CYP1A catalytic activity was assessed at the enzyme activity level-measured as 7-ethoxyresorufin-O-deethylase and cellular localization of CYP1A in the liver was studied using immunohistochemistry. Liver EROD was correlated with phenanthrene-type metabolites in liver and copper concentrations in water. Strong CYP1A occurrence was observed in acinar pancreatic cells, pancreatic duct epithelium and vascular system endothelium and negative/rare induction were observed in hepatocytes and sinusoidal endothelium. In gills, EROD activity showed a significant correlation with different fractions of heavy metals in sediment but no correlation was observed between EROD activity and PAHs. Strongly positive CYP1A associated staining of the vascular system endothelia and primary filament cells and a moderate staining of pillar cells in gills were observed. The results substantiated the utility of EROD activity and CYP1A induction measurement as biomarkers for use by aquatic toxicologists and indicate that catalytic assays and immunohistochemical assays appear to be sensitive to different kinds of pollutants being the use of both methods recommended for monitoring programs.

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“…The gills, primary routes of PAH uptake (Levine and Oris, 1999), have received less attention although they were proposed as more sensitive than the liver when comparing the induction of EROD activity, CYP1A protein or CYP1A transcripts in response to certain PAH (Abrahamson et al, 2007;Jönsson et al, 2006Jönsson et al, , 2010. Most recent findings have shown that gills EROD activity can provide a good indication of exposure to petroleum hydrocarbons in marine fishes, such as Atlantic cod Gadus morhua (Abrahamson et al, 2008;Holth et al, 2014) and polar cod Boreoga dussaida (Nahrgang et al, 2010), indicating that, probably, gills CYP1A metabolizes part of the absorbed PAH before it reaches the kidney and liver (Jönsson et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Gills CYP1A of Oncorhynchus mykiss as a sensitive biomarker of crude oil pollution in freshwater environments

Leggieri

Anna

Cárcamo

et al. 2019

Environmental Toxicology and Pharmacology

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The induction of CYP1A activity (EROD) and protein expression was compared in liver and gills of rainbow trout from a stream polluted with crude oil, and through laboratory exposures to 1% and 5% of water accommodated fraction of the crude oil (WAF) for 1 and 4 days. Gills EROD increased 1.6-2.7-fold in fish from the polluted stream and during experiments, while liver EROD was induced only by 1% WAF at day 1 (1.5-fold). Contrastingly, crude oil pollution strongly induced both liver and gills CYP1A protein expression in the field (14-36-fold) and in experiments (4-25fold). This highlights that crude oil induced CYP1A activity markedly in gills but only slightly or not 2 at all in the liver, suggesting that differences between organ EROD activities are related to the modulation of CYP1A enzyme activity but not to the regulation at transcriptional or translational levels.

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EROD activity in liver and gills of polar cod (Boreogadus saida) exposed to waterborne and dietary crude oil

Cited by 20 publications

References 18 publications

DNA damage and health effects in juvenile haddock (Melanogrammus aeglefinus) exposed to PAHs associated with oil-polluted sediment or produced water

DNA damage and health effects in juvenile haddock (Melanogrammus aeglefinus) exposed to PAHs associated with oil-polluted sediment or produced water

EROD activity and cytochrome P4501A induction in liver and gills of Senegal sole Solea senegalensis from a polluted Huelva Estuary (SW Spain)

Gills CYP1A of Oncorhynchus mykiss as a sensitive biomarker of crude oil pollution in freshwater environments

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