Errata: Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation<i>in vivo</i>

Dancik, Yuri; Favre, Amandine; Loy, Chong Jin; Zvyagin, Andrei V.; Roberts, Michael S.

doi:10.1117/1.jbo.18.2.029802

Cited by 3 publications

(3 citation statements)

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“…The application of FLIm to detect AF of tissues has been rapidly expanding from characterizing cells in monolayer culture to analysis of tissue biopsies such as skin , and engineered tissue constructs. , The main advantage of this approach is that samples can be imaged and analyzed without the need for additional labeling. FLIm is effective at identifying the unique AF signatures of collagen and collagen cross-links, which occur during tissue maturation. , Collagen expression and content are frequently used as markers for osteogenic differentiation and indicators of tissue maturation.…”

Section: Discussionmentioning

confidence: 99%

Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts

Harvestine

Sheaff

Cai

et al. 2019

ACS Biomater. Sci. Eng.

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There is a critical need to develop noninvasive, nondestructive methods for assessing the quality of engineered constructs prior to implantation. Currently, the composition and maturity of engineered tissues are assessed using destructive, costly, and time-consuming biochemical and mechanical analyses. The goal of this study was to use noninvasive, multimodal imaging to monitor osteogenic differentiation and matrix deposition by human mesenchymal stem/stromal cells (MSCs) during in vitro culture. MSCs were encapsulated in alginate hydrogels and cultured in osteogenic conditions for 4 weeks. Samples were evaluated using fluorescence lifetime imaging (FLIm) and ultrasound backscatter microscopy (UBM) prior to traditional biochemical and mechanical testing. Using linear regression analysis, we identified strong correlations between imaging parameters (e.g., fluorescence lifetime and acoustic attenuation coefficient) and destructive mechanical and biochemical tests to assess the maturation of osteogenically induced constructs. These data demonstrate the promise of nondestructive label-free imaging techniques to noninvasively ascertain the progression and maturity of tissue engineered bone grafts.

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Section: Discussionmentioning

confidence: 99%

Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts

Harvestine

Sheaff

Cai

et al. 2019

ACS Biomater. Sci. Eng.

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show abstract

“…The reader can have an insight on the in vivo work that started more than 20 years ago and at the evolution of the imaging instruments by consulting the references [2,6,8,16,. The first multiphoton/ FLIM data of human skin in vivo were obtained in the 1990s by and Masters et al with a homemade microscope on the forearm of one of the authors [2].…”

Section: Multiphoton Fluorescence Lifetime Imaging Of In Vivo Human Skinmentioning

confidence: 99%

“…The skin endogenous fluorophores present lifetimes on the order of hundreds of picoseconds (e.g. melanin, free NADH, bound FAD) to nanoseconds (see [16] and included references). The fluorescence lifetime is independent of fluorophore concentration, but depends on the local microenvironment of the molecule that is, for example, pH, binding status, conformational changes.…”

Section: Multiphoton Fluorescence Lifetime Imaging Of In Vivo Human Skinmentioning

confidence: 99%