Erratum: Multitarget super-resolution microscopy with high-density labeling by exchangeable probes

Abstract

In the version of this article initially published, the sequence reported for the dBrainbow construct was incorrect. The blue fluorescent protein was reported as EBFP2; it is mTFP1. The mKO2 protein is tagged with a V5 epitope and not a Myc epitope, and the EGFP protein is tagged with HSV, not with V5, as detailed in a previous correction to this paper. The red channel reflects endogenous mKO2 fluorescence, not anti-Myc staining. The mKO2 protein is produced by a codon-optimized sequence. These errors have been corrected in the HTML and PDF versions of the article, and the correct sequence is provided.Erratum: Rational design of a high-affinity, fast, red calcium indicator R-CaMP2 In the version of this article initially published, GenBank/EMBL/DDBJ accession codes for R-GECO2L and R-CaMP2 were omitted. These accession codes are KR149061 and KR149062, respectively. The error has been corrected in the HTML and PDF versions of the article.Erratum: Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy-treated macaques In the PDF version of this article initially published, the Acknowledgments section erroneously listed the Uehara Memorial Foundation as a funder twice. The error has been corrected in the PDF version of this article.Erratum: Power and sample size In the version of this article initially published, the terms "sensitivity" and "specificity" and the related descriptors "sensitive" and "specific" were mistakenly switched in three instances. The errors have been corrected in the HTML and PDF versions of the article.

When Weak Is Strong: A Plea for Low‐Affinity Binders for Optical Microscopy

“…[13] A second group are molecules that consist of a lowaffinity target-binding unit conjugated to a fluorophore. This group comprises, among others, conjugates of the DNA minor groove binder Hoechst with fluorophores, which were used for both PAINT [14] and STED microscopy; [2] fluorophore-peptide labels, such as the Lifeact peptide that targets cellular actin [15] and fluorophore-aptamer conjugates. [16] An interesting perspective is the use of physiological weak…”