Erratum: SWELL1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis

Zhang, Yanhui; Xie, Litao; Gunasekar, Susheel K.; Tong, Dan; Mishra, Anil; Gibson, William J.; Wang, Chuansong; Fidler, Trevor P.; Marthaler, Brodie; Klingelhutz, Aloysius J.; Samuel, Isaac; Smith, Jessica; Cao, Lei; Sah, Rajan

doi:10.1038/ncb3556

Cited by 6 publications

(4 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies proposed several candidates for mediating I Cl.vol ., including the transporter P-glycoprotein, the ICln,protein phospholemman, the voltage-dependent anion channel (VDAC), the chloride channel ClC-3 and the calciumactivated chloride channel TMEM16A (Duan et al, 1997;Pedersen et al, 2015). Recent studies have demonstrated that the protein, leucine-rich repeat containing 8A (LRRC8A, also called SWELL1) mediates I Cl.vol in the human cancer cell lines HCT166 cells and HeLa cells (Voss et al, 2014), human CD4 + T lymphocytes and B cells (Qiu et al, 2014), and mouse adipocyte (Zhang et al, 2017). Functional channels are thought to result from expression of LRRC8A along with several homologous subunits in varying combinations (Gradogna et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Noradrenaline up‐regulates volume‐regulated chloride current by PKA‐independent cAMP/exchange protein activated by cAMP pathway in human atrial myocytes

Xiao

Zhang

Wang

et al. 2018

British J Pharmacology

View full text Add to dashboard Cite

These data show that I evoked by cell swelling of human atrial myocytes is up-regulated by noradrenaline via a PKA-independent cAMP/Epac pathway in human atrial myocytes. cAMP/Epac-induced I is expected to shorten action potential duration during human atrial myocytes swelling and may be involved in abnormal cardiac electrical activity during cardiac pathologies that evoke β-adrenoceptor signalling.

show abstract

Section: Discussionmentioning

confidence: 99%

Noradrenaline up‐regulates volume‐regulated chloride current by PKA‐independent cAMP/exchange protein activated by cAMP pathway in human atrial myocytes

Xiao

Zhang

Wang

et al. 2018

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…Western blotting studies were carried out with lysates prepared from MIN6 cells by using standard techniques 63 . The primary antibodies used are listed above in antibodies and ion channel modulators.…”

Section: Methodsmentioning

confidence: 99%

“…Swell1 fl/fl mice were generated as previously described 63 . Briefly, Swell1 intronic sequences were obtained from Ensembl Transcript ID ENSMUST00000139454.…”

Section: Methodsmentioning

confidence: 99%

SWELL1 is a glucose sensor regulating β-cell excitability and systemic glycaemia

et al. 2018

Self Cite

View full text Add to dashboard Cite

Insulin secretion is initiated by activation of voltage-gated Ca2+ channels (VGCC) to trigger Ca2+-mediated insulin vesicle fusion with the β-cell plasma membrane. The firing of VGCC requires β-cell membrane depolarization, which is regulated by a balance of depolarizing and hyperpolarizing ionic currents. Here, we show that SWELL1 mediates a swell-activated, depolarizing chloride current (ICl,SWELL) in both murine and human β-cells. Hypotonic and glucose-stimulated β-cell swelling activates SWELL1-mediated ICl,SWELL and this contributes to membrane depolarization and activation of VGCC-dependent intracellular calcium signaling. SWELL1 depletion in MIN6 cells and islets significantly impairs glucose-stimulated insulin secretion. Tamoxifen-inducible β-cell-targeted Swell1 KO mice have normal fasting serum glucose and insulin levels but impaired glucose-stimulated insulin secretion and glucose tolerance; and this is further exacerbated in mild obesity. Our results reveal that β-cell SWELL1 modulates insulin secretion and systemic glycaemia by linking glucose-mediated β-cell swelling to membrane depolarization and activation of VGCC-triggered calcium signaling.

show abstract

“…Spontaneous lipid accumulation in preadipocytes, occurring on the BC platform without induction of differentiation, is a lower-cost and simpler solution suitable for a wide range of purposes. Notably, the spontaneously generated adipocytes on BC had the same range of diameters as mature adipocytes found in rats (45-50 µm [31]). The adipogenic differentiation of preadipocytes on BC may be related to two factors: (a) multidimensional cell distribution within the BC and (b) special mechanical cues in the soft environment of BC.…”

Section: Discussionmentioning

confidence: 66%

In vitro adipogenesis and long-term adipocyte culture in adipose tissue-derived cell banks

Chen¹,

Wang²,

Wang³

et al. 2021

Biofabrication

View full text Add to dashboard Cite

There is a critical need to develop in vitro culture systems appropriate for the expansion of adipose tissue, in order to gain new insights into metabolic diseases and to assist in the restoration of tissue defects. Conventional two- or three-dimensional (2D or 3D) in vitro models of adipocytes require a combination of supplements to induce adipocyte maturation that greatly increases the cost of large-scale industrial production. In the present study, a microporous, perforated bacterial cellulose (BC)-assisted culture system was developed that promoted the adhesion, proliferation, and adipogenic differentiation of preadipocytes. Additionally, the system maintained the cells as mature unilocular adipocytes ex vivo in normal cell culture medium in long-term culture. All cells were derived from isolated adipose tissue without the use of expensive enzymes for tissue digestion. In contrast to culture in hard tissue culture plates, preadipocytes in the soft 3D environments formed multidimensional interlaced cell contacts, undergoing significant spontaneous lipid accumulation and could be cultured for up to threemonths in maintenance medium. More importantly, the cultured adipose tissue-derived cell bank created here was able to produce injury repair activators that promoted the proliferation of fibroblasts with little fibrosis and the functional differentiation of myoblasts, displaying the potential for use in adipose reconstruction. Thus, the present study demonstrates the potential of a mechanically flexible BC scaffold to generate volume tunable adipose constructs and provides a low-cost and user-friendly strategy for large-scale industrial production of adipose tissue.

show abstract

Erratum: SWELL1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis

Abstract: © 2 0 1 7 M a c m i l l a n P u b l i s h e r s L i m i t e d , p a r t o f S p r i n g e r N a t u r e . A l l r i g h t s r e s e r v e d .

Cited by 6 publications

References 1 publication

Noradrenaline up‐regulates volume‐regulated chloride current by PKA‐independent cAMP/exchange protein activated by cAMP pathway in human atrial myocytes

Noradrenaline up‐regulates volume‐regulated chloride current by PKA‐independent cAMP/exchange protein activated by cAMP pathway in human atrial myocytes

SWELL1 is a glucose sensor regulating β-cell excitability and systemic glycaemia

In vitro adipogenesis and long-term adipocyte culture in adipose tissue-derived cell banks

Contact Info

Product

Resources

About