Erregerspektrum und Resistenzsituation bei rein anaeroben odontogenen Infektionen

Eckert, Alexander W.; Höhne, C; Schubert, Johannes

doi:10.1007/s100060050188

Cited by 16 publications

(10 citation statements)

References 24 publications

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“…Odontogenic infections are usually polymicrobial endogenous infections that are generally well amenable to empirical antibiotic therapy. However, as in other fields of medicine, resistance to antibiotics has been increasingly observed in the recent past, which can occasionally complicate such empirical antibiotic therapy [ 7 , 8 ]. Particularly in extensive infections, cultural pathogen diagnostics are performed to enable targeted antibiotic treatment according to an antibiotic susceptibility profile, especially if the initial therapeutic interventions are not optimally effective [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Numerous authors have shown that odontogenic infections are mainly caused by anaerobic bacteria [ 7 , 9 , 10 ]. However, these are very difficult to detect culturally [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Numerous authors have shown that odontogenic infections are mainly caused by anaerobic bacteria [ 7 , 9 , 10 ]. However, these are very difficult to detect culturally [ 7 ]. Therefore, high demands must be placed on pre-analytic considerations such as sampling, the transport medium, and the entire time sequence until pathogen diagnostics in the laboratory.…”

Section: Introductionmentioning

confidence: 99%

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Clinical Relevance of the Microbiome in Odontogenic Abscesses

Böttger

Zechel-Gran

Schmermund

et al. 2021

Biology

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Odontogenic abscesses are usually caused by bacteria of the oral microbiome. However, the diagnostic culture of these bacteria is often prone to errors and sometimes fails completely due to the fastidiousness of the relevant bacterial species. The question arises whether additional pathogen diagnostics using molecular methods provide additional benefits for diagnostics and therapy. Experimental 16S rRNA gene analysis with next-generation sequencing (NGS) and bioinformatics was used to identify the microbiome of the pus in patients with severe odontogenic infections and was compared to the result of standard diagnostic culture. The pus microbiome was determined in 48 hospitalized patients with a severe odontogenic abscess in addition to standard cultural pathogen detection. Cultural detection was possible in 41 (85.42%) of 48 patients, while a pus-microbiome could be determined in all cases. The microbiomes showed polymicrobial infections in 46 (95.83%) cases, while the picture of a mono-infection occurred only twice (4.17%). In most cases, a predominantly anaerobic spectrum with an abundance of bacteria was found in the pus-microbiome, while culture detected mainly Streptococcus, Staphylococcus, and Prevotella spp. The determination of the microbiome of odontogenic abscesses clearly shows a higher number of bacteria and a significantly higher proportion of anaerobes than classical cultural methods. The 16S rRNA gene analysis detects considerably more bacteria than conventional cultural methods, even in culture-negative samples. Molecular methods should be implemented as standards in medical microbiology diagnostics, particularly for the detection of polymicrobial infections with a predominance of anaerobic bacteria.

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Section: Introductionmentioning

confidence: 99%

“…Numerous authors have shown that odontogenic infections are mainly caused by anaerobic bacteria [ 7 , 9 , 10 ]. However, these are very difficult to detect culturally [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clinical Relevance of the Microbiome in Odontogenic Abscesses

Böttger

Zechel-Gran

Schmermund

et al. 2021

Biology

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“…Антибактериальная терапия остается неотъемлемой составляющей стратегии лечения инфекционной патологии [3,12]. Однако нерациональное применение антибиотиков является причиной появления резистентных штаммов микроорганизмов, устойчивых к их действию [8,9].…”

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Rational antimicrobial therapy in patients with purulent inflammatory diseases of maxillofacial area

Bogatov

Denis

Koliadov

2015

Stomat.

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The aim of the study was to reveal optimal antimicrobial agents in patients with purulent inflammatory diseases of maxillofacial area according to bacteriological study results. One hundred twenty-one culture samples from 32 patients aged from 21 to 68 years admitted to Nurnberg Maxillofacial Surgery Clinic. Pathogenic flora was mostly mixed with 66.9% of anaerobic germs in the majority of cases irresponsible to ampicillin (1.3%) and clindamycin (0.7%). The drugs of choice are ampicillin/sulbactam and amoxicillin/clavulanate. Bacteriological study should be included as a useful tool in treatment protocols of patients with purulent inflammatory diseases of maxillofacial area.

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“…), or Prevotella (spp. ), we observed that almost 56% of the DNA originated from the genera Alcanivorax , Delftia [ 9 , 29 , 30 ], and Porphyromonas . According to our research, no human pathogenicity has yet been demonstrated for Alcanivorax while numerous diseases have been linked to Delftia , especially to Delftia acidovorans .…”

Section: Discussionmentioning

confidence: 99%

A New Type of Chronic Wound Infection after Wisdom Tooth Extraction: A Diagnostic Approach with 16S-rRNA Gene Analysis, Next-Generation Sequencing, and Bioinformatics

et al. 2020

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Delayed-onset infections are rare postoperative complications of lower third molar extractions. This article presents a case of a chronic combined hard and soft tissue infection after the extraction of a third molar, where the causative organisms could only be elucidated by molecular methods. Experimental 16S-rRNA gene analysis with next-generation sequencing and bioinformatics was used to identify the bacterial spectrum of the infection. 16S-rRNA gene analysis delivered the microbiome of the abscessing inflammation while standard culture and laboratory examinations were all sterile. The microbiome showed a mixed bacterial infection with a dominance of Delftia and Alcanivorax (spp.) besides other bacteria of the normal oral flora. Using 16S-rRNA-gene analysis, next-generation sequencing, and bioinformatics, a new type of chronic wound infection after wisdom tooth extraction was found. The property of Delftia and Alcanivorax (spp.) as water-affine environmental bacteria raises suspicion of infection from contaminated water from a dental unit. Thus, osteotomies of teeth should only be done with sterile cooling water. The 16S-rRNA gene analysis should become a part of the routine diagnostics in medical microbiology.

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Erregerspektrum und Resistenzsituation bei rein anaeroben odontogenen Infektionen

Cited by 16 publications

References 24 publications

Clinical Relevance of the Microbiome in Odontogenic Abscesses

Clinical Relevance of the Microbiome in Odontogenic Abscesses

Rational antimicrobial therapy in patients with purulent inflammatory diseases of maxillofacial area

A New Type of Chronic Wound Infection after Wisdom Tooth Extraction: A Diagnostic Approach with 16S-rRNA Gene Analysis, Next-Generation Sequencing, and Bioinformatics

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