Erwinia chrysanthemi: description of two new biovars (bv 8 and bv 9) isolated from kalanchoe and maize host plants

Ngwira, N.; Samson, R.

doi:10.1051/agro:19900408

Cited by 32 publications

(36 citation statements)

References 9 publications

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“…Weak utilisation of β-gentiobiose, was not a distinguishing feature since biovar 3 strains are variable with respect to utilisation of this substrate. Results from the biochemical assays on reference strains, conducted in microtiter plates, agreed largely with those expected according to Ngwira and Samson (1990) and PalacioBielsa et al (2006). For biovar 1 and 7 strains, however, no assimilation of inulin was observed.…”

Section: Discussionsupporting

confidence: 86%

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Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe

Slawiak

Beckhoven²,

Speksnijder³

et al. 2009

Eur J Plant Pathol

188

185

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Sixty-five potato strains of the soft rotcausing plant pathogenic bacterium Dickeya spp., and two strains from hyacinth, were characterised using biochemical assays, REP-PCR genomic finger printing, 16S rDNA and dnaX sequence analysis. These methods were compared with nineteen strains representing six Dickeya species which included the type strains. A group of twenty-two potato strains isolated between 2005-2007 in the Netherlands, Poland, Finland and Israel were characterised as belonging to biovar 3. They were 100% identical in REP-PCR, dnaX and 16S rDNA sequence analysis. In a polyphasic analysis they formed a new clade different from the six Dickeya species previously described, and may therefore constitute a new species. The strains were very similar to a Dutch strain from hyacinth. On the basis of dnaX sequences and biochemical assays, all other potato strains isolated in Europe between 1979 and 1994 were identified as D. dianthicola (biovar 1 and 7), with the exception of two German strains classified as D. dieffenbachia (biovar 2) and D. dadantii (biovar 3), respectively. Potato strains from Peru were classified as D. dadantii, from Australia as D. zeae and from Taiwan as D. chrysanthemi bv. parthenii, indicating that different Dickeya species are found in association with potato.

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Section: Discussionsupporting

confidence: 86%

“…This classification is partly correlated with the former distribution in biochemically distinct groups (biovars) described by Ngwira and Samson (1990). Studies in the past indicated that European potato strains often belonged to biovars 1 and 7, which coincide with D. dianthicola, a species adapted to cool regions (Janse and Ruissen 1988).…”

Section: Introductionmentioning

confidence: 69%

Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe

Slawiak

Beckhoven²,

Speksnijder³

et al. 2009

Eur J Plant Pathol

188

185

View full text Add to dashboard Cite

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“…Dickeya strains were characterised by biochemical tests according to Samson et al (1987) and Ngwira and Samson (1990). Briefly, the bacteria were grown in King's B medium for 24-48 h, collected by centrifugation and resuspended in sterile water.…”

Section: Biovar Determinationmentioning

confidence: 99%

Symptoms and yield reduction caused by Dickeya spp. strains isolated from potato and river water in Finland

et al. 2009

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Biochemical characterisation of Dickeya strains isolated from potato plants and river water samples in Finland showed that the majority of the strains were biovar 3. They thus resembled the strains recently isolated from potato in the Netherlands, Poland and Israel and form a new clade within the Dickeya genus. About half of the Finnish isolates resembling strains within this new clade were virulent and caused wilting, necrotic lesions and rotting of leaves and stems. Similar symptoms were caused by D. dianthicola strains isolated from one potato sample and from several river water samples. Frequently, the rotting caused by the Dickeya strains was visible in the upper parts of the stem, while the stem base was necrotic from the pith but hard and green on the outside, resulting in symptoms quite different from the blackleg caused by Pectobacterium atrosepticum. The presence of Dickeya in the symptomatic plants in the field assay was verified with a conventional PCR and with a real-time PCR test developed for the purpose. The virulent Dickeya strains reduced the yield of individual plants by up to 50% and caused rotting of the daughter tubers in the field and in storage. Management of Dickeya spp. in the potato production chain requires awareness of the symptoms and extensive knowledge about the epidemiology of the disease.

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“…Erwinia chrysanthemi) has been subdivided into biovars using 10 biochemical and physiological tests (Samson et al, 1987 ;Ngwira & Samson, 1990). Five of these tests (growth at 39 mC, acid production from -arabinose, melibiose and raffinose, and assimilation of 5-keto--gluconate) were confirmed to be pertinent for distinguishing reference strains of Pectobacterium chrysanthemi biovars (Table 2).…”

Section: Numerical Analysis Of Phenotypic Characteristicsmentioning

confidence: 99%

“…Hydrolysis of gelatin, lecithin and casein was tested on gelatin agar, on egg-yolk agar and on skimmed-milk agar, respectively (Smibert & Krieg, 1994). Acid production from inulin in phenol red peptone water, production of reducing substances from sucrose, malonate utilization, indole production from tryptophan, anaerobic degradation of arginine (Moeller medium) and utilization of citrate as sole carbon source (Simmons' medium) were studied using previously described methods (Ngwira & Samson, 1990 ;Gallois et al, 1992). Liquefaction of calcium polygalacturonate was tested in Sutton 's medium modified after Bonnet (1973).…”

Section: Introductionmentioning

confidence: 99%

Samsonia erythrinae gen. nov., sp. nov., isolated from bark necrotic lesions of Erythrina sp., and discrimination of plant-pathogenic Enterobacteriaceae by phenotypic features.

Sutra¹,

Christen²,

Bollet³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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Bacterial strains isolated from diseased erythrina (Erythrina sp.) trees in Martinique (French West Indies) were studied using phenotypic tests, 16S rDNA sequence analysis and DNA-DNA hybridization. Numerical analysis of phenotypic characteristics showed that these strains formed an homogeneous phenon among plant-pathogenic Enterobacteriaceae, and gave useful and updated information for the identification of these bacteria. Results of DNA-DNA hybridization indicated that strains from erythrina belonged to a discrete genomospecies (89-100 % hybridization) and had low levels of DNA relatedness (2-33 % hybridization) with reference strains of phytopathogenic Erwinia, Brenneria, Pectobacterium, Pantoea and Enterobacter species. 16S rDNA sequence analysis using three different methods revealed that the position of strain CFBP 5236 T isolated from erythrina was variable in the different trees, so that strains from erythrina could not be assigned to any recognized genus. It is proposed that these strains are included in a new genus, Samsonia. The name Samsonia erythrinae is proposed for the new species. The GMC content of the DNA of the type strain, CFBP 5236 T (l ICMP 13937 T ), is 570 mol %.

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Erwinia chrysanthemi: description of two new biovars (bv 8 and bv 9) isolated from kalanchoe and maize host plants

Cited by 32 publications

References 9 publications

Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe

Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe

Symptoms and yield reduction caused by Dickeya spp. strains isolated from potato and river water in Finland

Samsonia erythrinae gen. nov., sp. nov., isolated from bark necrotic lesions of Erythrina sp., and discrimination of plant-pathogenic Enterobacteriaceae by phenotypic features.

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