Erythrocyte adenylate kinase isoenzyme as a marker for hemolysis

Thomas, George; Murthy, Vadiraja V.

doi:10.1002/(sici)1098-2825(1997)11:6<351::aid-jcla8>3.0.co;2-8

Cited by 5 publications

(3 citation statements)

References 4 publications

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“…Isoprene exhalation seemed to be linked to intravascular haemolysis as isoprene exhalation profiles were similar to changes in LDH serum levels during HD. Haemolysis has been known to be a complication of renal replacement therapy for a long time and is due to mechanical damage in erythrocytes produced by extracorporeal blood circulation through contact of blood with foreign particle surfaces [37][38][39]. LDH liberated from the erythrocytes can then be determined in serum.…”

Section: Discussionmentioning

confidence: 99%

Volatile breath biomarkers for patient monitoring during haemodialysis

et al. 2013

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Patients with end-stage renal disease (ESRD) are at risk for a numerous complications. This study was intended to evaluate breath analysis for monitoring and therapy initiation under haemodialysis (HD). Exhaled alveolar air from 30 ESRD patients during 4 h thrice-weekly HD was analysed by means of HS-SPME-GC-MS. Venous blood samples were taken for determination of conventional serum parameters. Exhaled concentrations of isoprene (10-589 ppbV) were dropped at initiation of HD and increased at the end of HD. Isoprene concentration changes were similar to changes of serum LDH activities. Variation of exhaled acetone concentrations (59 to 8509 ppbV) was significantly lower in diabetic patients when compared to non-diabetics. Exhaled pentane (0.3 to 12 ppbV) increased at onset of HD and returned to baseline levels afterwards. Benzene concentrations showed typical washout characteristics. Ethanol and DMS concentrations remained constant during HD. Breath analysis can be used to recognize oxidative stress, metabolic conditions and haemolysis during HD. Hence, non-invasive breath testing could be used to monitor ESRD patients under HD and prevent them from being affected by well-known detrimental side effects of renal replacement therapy.

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Section: Discussionmentioning

confidence: 99%

Volatile breath biomarkers for patient monitoring during haemodialysis

et al. 2013

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“…, 1983; Wolfe, 1985; Lerche & Merk, 1996; Shimono et al. , 1996; Thomas & Murthy, 1997; Hansen et al. , 1998).…”

Section: Discussionmentioning

confidence: 99%

Feasibility of a new in vitro approach to evaluate cellular damage following co-infusion of red blood cell concentrates and intravenous drug solutions

Bos

Wissink

Schattenberg

et al. 2003

Clinical &Amp; Laboratory Haematology

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A novel in vitro dynamic infusion system appears to represent a useful technique to calculate possible damage to RBCs resulting from co-infused drug solutions. Co-infusion of the drug solutions tested with filtered and irradiated RBC concentrates did not produce fHb levels above the levels accepted by the Dutch national guidelines. Apart from haemolysis, other parameters reflecting RBC damage should be investigated in future studies.

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“…Adenylate kinase (AK, EC 2.7.4.3) catalyzes the reaction MgATP+AMP⇔MgADP+ADP, which is essential for cell survival [3–10]. This small kinase, containing two cysteine residues and no disulfide bond, has also been considered a ‘model kinase’ in the study of structure–function relationship of kinases [11].…”

Section: Introductionmentioning

confidence: 99%

Cysteine‐25 of adenylate kinase reacts with dithiothreitol to form an adduct upon aging of the enzyme

Li¹,

Han²,

Pan³

2001

FEBS Letters

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Adenylate kinase (AK) ages in solution in the presence of DL-dithiothreitol (DTT) with a gradual activity decrease. Upon dilution with 4 M guanidine hydrochloride denatured native and aged AK, both recover to the same activity as the fresh enzyme. Mass spectroscopy and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole chloride modification kinetics studies identify that the residue cysteine-25 of the enzyme reacts with DTT to form an adduct. The formation of the unusual bridging DTT adduct of AK appears to be the result of a stable DTT^protein complex. The K M for AMP, ADP and MgATP of the DTT-modified enzyme does not differ significantly from that of the intact enzyme, whereas the secondary and tertiary structures of the enzyme change obviously. These results indicate that cysteine-25 may not be involved directly in substrate binding, but may play an important role in maintaining secondary and tertiary structures of native AK, as well as the conformation interconversion in the catalytic cycle. ß

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Erythrocyte adenylate kinase isoenzyme as a marker for hemolysis

Cited by 5 publications

References 4 publications

Volatile breath biomarkers for patient monitoring during haemodialysis

Volatile breath biomarkers for patient monitoring during haemodialysis

Feasibility of a new in vitro approach to evaluate cellular damage following co-infusion of red blood cell concentrates and intravenous drug solutions

Cysteine‐25 of adenylate kinase reacts with dithiothreitol to form an adduct upon aging of the enzyme

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