Erythrocyte Binding Preference of Avian Influenza H5N1 Viruses

Louisirirotchanakul, Suda; Lerdsamran, Hatairat; Wiriyarat, Witthawat; Sangsiriwut, Kantima; Chaichoune, Kridsada; Pooruk, Phisanu; Songserm, Taweesak; Kitphati, Rungrueng; Sawanpanyalert, Pathom; Komoltri, Chulaluk; Auewarakul, Prasert; Puthavathana, Pilaipan

doi:10.1128/jcm.00921-07

Cited by 28 publications

(22 citation statements)

References 6 publications

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“…Our finding provides an alternative choice of erythrocytes that can be accessed more easily for Asian countries. We previously reported this finding in a smaller number of blood samples (5).…”

Section: Resultssupporting

confidence: 51%

“…The protocol as described previously employed ei- ther 1% horse or 0.5% goose erythrocytes (5,8). Even though horse erythrocytes have been recommended by previous investigators and WHO (8,11) for detection of antibody to AI virus by HI assay, we did not see a marked difference in the antibody titers obtained when either kind of erythrocytes was used (Table 2).…”

Section: Resultsmentioning

confidence: 61%

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Kinetics and Longevity of Antibody Response to Influenza A H5N1 Virus Infection in Humans

Kitphati

Pooruk

Lerdsamran

et al. 2009

Clin Vaccine Immunol

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Anti-H5N1 antibody was determined by microneutralization, hemagglutination inhibition, and Western blotting assays in serial blood samples collected from eight Thai patients, including four fatal cases and four survivors. The antibody was detected as early as 5 days and, typically, with an increase in titer in paired blood at about 15 days after disease onset. The anti-H5 antibody response was long-lasting, for almost 5 years in cases which can be followed that far. In addition, cross-neutralizing activity to related clade 1 viruses was observed.

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Section: Resultssupporting

confidence: 51%

Section: Resultsmentioning

confidence: 61%

Kinetics and Longevity of Antibody Response to Influenza A H5N1 Virus Infection in Humans

Kitphati

Pooruk

Lerdsamran

et al. 2009

Clin Vaccine Immunol

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“…In earlier epidemics, the H3N2 virus was found to agglutinate both chicken and guinea pig erythrocytes. However, the viral strain that became prominent during the 1992/1993 flu season lacked the chicken hemagglutination [Ch(-) virus] (8,10,11). In our study, the trypsin-or chymotrypsin-passaged Ch(-) virus produced lines with differing hemagglutination properties.…”

mentioning

confidence: 78%

Protease-Dependent Hemagglutinin Cleavage Contributes to Alteration in Chicken Hemagglutination by the H3N2 Influenza A Virus

et al. 2013

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SUMMARY:The human influenza A virus (H3N2) has been the predominant influenza strain since 1992, and one property of this virus is non-agglutination of chicken erythrocytes [Ch(-) virus]. The Ch(-) virus in our study was able to acquire chicken hemagglutination [Ch(＋)] by trypsin passage but not by chymotrypsin passage. Moreover, the trypsin-passaged Ch(＋) viruses reacquired the Ch(-) property after a further chymotrypsin passage. In particular, genetic analysis showed no evidence of mutations in the hemagglutinin (HA) gene during either trypsin or chymotrypsin passages: the only differences found were in the HA cleavage sites between the trypsin-passaged virus and the chymotrypsin-passaged virus as determined by the N-terminal amino acid sequence. These results suggested that protease-dependent differences at the viral HA cleavage site, rather than genetic mutations, are likely to have a significant effect on the viral ability to produce chicken hemagglutination.

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“…This receptor is also found on the RBC of several species of animals. Turkeys, guinea pigs, type O-human blood, and chicken RBC are traditionally used for HI tests (48)(49)(50). Due to the presentation of sialic acid receptor on RBC, IAVs can agglutinate RBC (34).…”

Section: Discussionmentioning

confidence: 99%

Neutralizing DNA Aptamers against Swine Influenza H3N2 Viruses

et al. 2013

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e Triple reassortant influenza A viruses (IAVs) of swine, particularly the North American H3N2 subtype, circulate in swine herds and may reassort and result in the emergence of novel zoonotic strains. Current diagnostic tools rely on isolation of the viruses, followed by serotyping by hemagglutination or genome sequencing, both of which can be expensive and time-consuming. Thus, novel subtype-specific ligands and methods are needed for rapid testing and subtyping of IAVs in the field. To address this need, we selected DNA aptamers against the recombinant HA protein from swine IAV H3 cluster IV using systematic evolution of ligands by exponential enrichment (SELEX). Four candidate aptamers (HA68, HA7, HA2a, and HA2b) were identified and characterized. The dissociation constants (K d ) of aptamers HA68, HA7, HA2a, and HA2b against recombinant H3 protein were 7.1, 22.3, 16.0, and 3.7 nM, respectively. The binding site of HA68 to H3 was identified to be between nucleotide residues 8 and 40. All aptamers inhibited H3 hemagglutination. HA68 was highly specific to all four lineages within the North American H3N2 subtype. Further, the other three aptamers specifically identified live viruses belonging to the phylogenetic clusters I, II/III, and IV especially the virus that closely related to the recent H3N2 variant (H3N2v). Aptamer HA68 was also able to bind and detect H3N2v isolated from recent human cases. In conclusion, we provide subtype-specific aptamers against H3N2 IAVs of swine that can now be used in rapid detection and typing protocols for field applications.

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Erythrocyte Binding Preference of Avian Influenza H5N1 Viruses

Cited by 28 publications

References 6 publications

Kinetics and Longevity of Antibody Response to Influenza A H5N1 Virus Infection in Humans

Kinetics and Longevity of Antibody Response to Influenza A H5N1 Virus Infection in Humans

Protease-Dependent Hemagglutinin Cleavage Contributes to Alteration in Chicken Hemagglutination by the H3N2 Influenza A Virus

Neutralizing DNA Aptamers against Swine Influenza H3N2 Viruses

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