Erythrocyte (Ca⁺²+Mg⁺²)‐ATPase activity: Increased sensitivity to oxidative stress in glucose‐6‐phosphate dehydrogenase deficiency

Abstract

The effect of the thiol-oxidizing agent diamide on erythrocyte (Ca+2 + Mg+2)-ATPase activity was measured in normal and glucose-6-phosphate-dehydrogenase-deficient (G6PD-) cells. Although the enzyme activity before the oxidative stress was similar in both groups, diamide induced a markedly greater inhibition in the enzyme activity in the G6PD- cells than in the normal controls. These data indicate dependence of erythrocyte (Ca+2 + Mg+2)-ATPase, in part, on the redox status of the cell. The increased vulnerability of (Ca+2 + Mg+2)-ATPase to oxidative stress in G6PD- may be of pathophysiological relevance to their premature destruction in oxidant-induced hemolysis.

Low-Grade Haemolysis and Assessment of Iron Status during the Steady State in G6PD-Deficient Subjects

“…Red blood cells with G6PD deficiency are unable to reduce NADP to NADPH at a normal rate; their capacity for regeneration of GSH is limited; and their pro teolytic systems are damaged [3][4][5].…”