Erythrocyte membrane lipid peroxidation in iron deficiency anemia

Ramachandran, M.; Iyer, G. Y. N.

doi:10.1007/bf01963584

Cited by 16 publications

(16 citation statements)

References 18 publications

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“…They suggested that increased antioxidant capacity was a compensatory factor for increased oxidant stress (16,18). In this study, total antioxidant capacity in patients with IDA was lower than controls.…”

Section: Discussionmentioning

confidence: 90%

Oxidative status in iron‐deficiency anemia

Yoo

Maeng

Sun

et al. 2009

Clinical Laboratory Analysis

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Oxidative stress is an imbalance between free radicals and antioxidant molecules that can play an important role in the pathogenesis of iron-deficiency anemia (IDA). The aim of this study was to investigate oxidative status in patients with IDA and alteration of oxidative status after iron treatment. Thirty-three female patients with IDA and 25 healthy controls were included in this study. Oxidant and total antioxidant capacity were determined using free oxygen radicals test and free oxygen radicals defence (Form CR 3000, Callegari, Parma, Italy). Catalase activity was measured by spectrophotometer using a commercially available kit (Bioxytech Catalase-520, OxisResearch, Portland, OR). Oxidant activity in patients with IDA was significantly higher than controls (P<0.05), while total antioxidant and catalase activity were significantly lower (P<0.05). After treatment, oxidant, antioxidant, and catalase activity reached the levels of the control group, and no significant differences were observed among groups (P>0.05). In conclusion, our data indicate that blood reactive oxygen species was lower and total antioxidant and catalase activity were higher after rather than before treatment in patients with IDA. The results of our study support the higher oxidative stress hypothesis in IDA; however, due to the limited number of cases included, more studies may be required to confirm the results.

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Section: Discussionmentioning

confidence: 90%

Oxidative status in iron‐deficiency anemia

Yoo

Maeng

Sun

et al. 2009

Clinical Laboratory Analysis

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“…The majority of studies on oxidative stress in IDA are based on lipid peroxidation measurements, and their results are contradictory. It has been determined that red blood cell susceptibility to in vitro oxidation is increased in IDA patients [4,32]; however, some authors have suggested that there is no evidence of increased susceptibility of red blood cells to lipid peroxidation [1,23]. Biomolecular targets of free radicals extend beyond lipids, and DNA oxidation may have more severe consequences.…”

Section: Discussionmentioning

confidence: 96%

Leukocyte DNA damage in children with iron deficiency anemia: effect of iron supplementation

et al. 2010

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Iron deficiency is frequently associated with anemia. Iron is a transition-metal ion, and it can induce free radical formation, which leads to formation of various lesions in DNA, proteins, and lipids. The aim of this study was to investigate baseline oxidative DNA damage and to clarify the role of the administration of a therapeutic dose of iron on DNA oxidation in children with iron deficiency anemia (IDA). Twenty-seven children with IDA and 20 healthy children were enrolled in the study. Leukocyte DNA damage (strand breaks and Fpg-sensitive sites) was assessed using comet assay before and after 12 weeks of daily iron administration. Before the iron administration, the frequency of DNA strand breaks in the children with IDA was found to be lower than those in the control group (P < 0.05), but there was not a significant difference for frequency of Fpg-sensitive sites. After 12 weeks of iron administration, the frequency of both DNA strand breaks and Fpg-sensitive sites were found to be increased (P < 0.01). No significant association was determined between DNA damage parameters and hemoglobin, hematocrit, serum iron, total iron binding capacity, and ferritin. In conclusion, basal level of DNA strand breaks is at a low level in children with IDA. After iron administration, DNA strand breaks and Fpg-sensitive sites, which represent oxidatively damaged DNA, increased. However, this increase was unrelated to serum level of iron and ferritin.

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“…Although conflicting results have been reported [8,10], it is generally accepted that oxidative stress is increased through increase in oxidant levels and/or decrease in antioxidant enzyme capacities in IDA. Generally, decreased GSH-Px activity along with normal or increased SOD and CAT activity are the main findings in IDA [10,11].…”

Section: Discussionmentioning

confidence: 99%

“…Oxidant levels and antioxidant enzyme activities such as SOD, GSH-Px and CAT have been evaluated in several studies [8][9][10][11][12][13][14]. Although conflicting results have been reported [8,10], it is generally accepted that oxidative stress is increased through increase in oxidant levels and/or decrease in antioxidant enzyme capacities in IDA.…”

Section: Discussionmentioning

confidence: 99%

“…Increased oxidative stress in IDA subjects was reported in several studies, in which the oxidative status was evaluated using measurement of oxidants, individual antioxidants, or both [8][9][10][11][12][13][14]. In these studies, it has been reported that oxidants were increased and antioxidants were decreased, and as a result, the oxidative/anti-oxidative balance shifted toward the oxidative side in patients with IDA.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of oxidative status in iron deficiency anemia through total antioxidant capacity measured using an automated method

Aslan¹,

Horoz²,

Çelik³

2011

Turk J Hematol

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Objective: Oxidative stress, an increase in oxidants and/or a decrease in antioxidant capacity, is one of the potential biochemical mechanisms involved in the pathogenesis of iron deficiency anemia. The objective of this study was to evaluate the oxidative status and to determine whether there is any relationship between oxidative status and the severity of anemia in patients with iron deficiency anemia using an automated method. Materials and Methods: Twenty-six subjects with iron deficiency anemia and 20 healthy controls were enrolled in the present study. Serum total antioxidant capacity, serum total peroxide level and oxidative stress index were determined in all study subjects. Results: Serum total antioxidant capacity was significantly lower in patients with iron deficiency anemia than controls (p<0.05), while serum total peroxide level and oxidative stress index were significantly higher (both p<0.05). There was a significant correlation between hemoglobin level and serum total peroxide level, oxidative stress index and total antioxidant capacity (r=-0.504, p<0.05; r=-0.503, p<0.05; r=0.417, p<0.05, respectively). Conclusion: Increased oxidative stress may play a role in the pathogenesis of iron deficiency anemia. Supplementation of antioxidant vitamins in conjunction with iron replacement therapy may offer better responses and provide early resolution of symptoms related to iron deficiency anemia. The automated assay is a reliable and easily applied method for measurement of serum total antioxidant capacity in iron deficiency anemia. (Turk J Hematol 2011; 28: 42-6)

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Erythrocyte membrane lipid peroxidation in iron deficiency anemia

Cited by 16 publications

References 18 publications

Oxidative status in iron‐deficiency anemia

Oxidative status in iron‐deficiency anemia

Leukocyte DNA damage in children with iron deficiency anemia: effect of iron supplementation

Evaluation of oxidative status in iron deficiency anemia through total antioxidant capacity measured using an automated method

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