Erythrocyte reduced/oxidized glutathione and serum thiol/disulfide homeostasis in patients with rheumatoid arthritis

Alışık, Murat; Alışık, Tuğba; Nacır, Barış; Neşeli̇oğlu, Salim; Genc-Isik, Irem; Koyuncu, Pinar; Erel, Özcan

doi:10.1016/j.clinbiochem.2021.04.023

Cited by 13 publications

(10 citation statements)

References 33 publications

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“…It is indeed expectable that the severity of RA disease could be associated to a decreased TAC. The increased systemic oxidative stress condition, such as that occurring in tissues of RA patients ( Kundu et al, 2012 ; Veselinovic et al, 2014 ; Quiñonez-Flores et al, 2016 ), could per se favor a RBCs pro-oxidant leading to the alteration of intracellular redox state and to the decrease of the intracellular antioxidant defense, such as glutathione ( Alisi et al, 2021 ). Similarly, the negative correlation between ER-α expression and TAC indirectly links the increase of oxidative stress and the RBCs activation mediated by ER-α pathway which could further contribute to the establishment and promotion of the systemic pro-oxidant status of RBCs confirming their role as risk factors for CVD ( Vona et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Estrogen receptors, ERK1/2 phosphorylation and reactive oxidizing species in red blood cells from patients with rheumatoid arthritis

Franco

Vona²,

Gambardella³

et al. 2022

Front. Physiol.

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Red blood cells (RBCs) are recognized to be important pathogenetic determinants in several human cardiovascular diseases (CVD). Undergoing to functional alterations when submitted to risk factors, RBCs modify their own intracellular signaling and the redox balance, shift their status from antioxidant defense to pro-oxidant agents, become a potent atherogenic stimulus playing a key role in the dysregulation of the vascular homeostasis favoring the developing and progression of CVD. Rheumatoid arthritis (RA) is a chronic autoimmune disease associated with a significantly increased risk of cardiovascular mortality with a prevalence from two to five more likely in woman, mainly attributed to accelerated atherosclerosis. The purpose of this study was to correlate the RA disease activity and the RBCs functional characteristics. Thirty-two women (aged more than 18 years) with RA, and 25 age-matched healthy women were included in this study. The disease activity, measured as the number of swollen and painful joints (DAS-28), was correlated with 1) the expression of RBCs estrogen receptors, which modulate the RBC intracellular signaling, 2) the activation of the estrogen-linked kinase ERK½, which is a key regulator of RBC adhesion and survival, and 3) the levels of inflammatory- and oxidative stress-related biomarkers, such as the acute-phase reactants, the antioxidant capacity of plasma, the reactive oxidizing species formation and 3-nitrotyrosine. All the biomarkers were evaluated in RA patients at baseline and 6 months after treatment with disease-modifying anti-rheumatic drugs (DMARDs). We found, for the first times, that in RA patients 1) the DAS-28 correlated with RBC ER-α expression, and did not correlate with total antioxidant capacity of plasma; 2) the RBC ER-α expression correlated with systemic inflammatory biomarkers and oxidative stress parameters, as well as ERK½ phosphorylation; and 3) the DMARDs treatments improved the clinical condition measured by DAS-28 score decrease, although the RBCs appeared to be more prone to pro-oxidant status associated to the expression of survival molecules. These findings represent an important advance in the study of RA determinants favoring the developing of CVD, because strongly suggest that RBCs could also participate in the vascular homeostasis through fine modulation of an intracellular signal linked to the ER-α.

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Section: Discussionmentioning

confidence: 99%

Estrogen receptors, ERK1/2 phosphorylation and reactive oxidizing species in red blood cells from patients with rheumatoid arthritis

Franco

Vona²,

Gambardella³

et al. 2022

Front. Physiol.

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“… 2 Abnormal levels of GSH and GSSG are associated with many clinical conditions, such as Alzheimer's disease, liver damage, and heart disease. 3,4 Therefore, GSH is widely used in the pharmaceutical, cosmetics and other industries. Based on its important biological functions, the measurement of its internal parameters is greatly important for health diagnosis.…”

Section: Instructionmentioning

confidence: 99%

“… 16,17 It has been proved that DNA bio-dots made from cytosine-rich DNA forms sp 2 carbon sample centers through the accumulation of aromatic cytosine base pairs. 3,18,19 Not only does it have a luminescent behavior similar to carbon quantum dots (CQDs), but it also owns a DNA-like structure. 18,20 These characteristics make it easier to extend to the construction of fluorescent biosensors.…”

Section: Instructionmentioning

confidence: 99%

Turn-on signal fluorescence sensor based on DNA derived bio-dots/polydopamine nanoparticles for the detection of glutathione

Chen

et al. 2022

RSC Adv.

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“…7 Thus, while SH level in the environment is an antioxidant marker, the level of SS formed is considered as an indicator of oxidative stress marker. 8 A large part of the plasma or serum thiol pool is formed by the SH groups of proteins such as albumin. 9 However, the majority of the thiol pool of the intracellular is composed of glutathione, which is the major and the most abundant antioxidant molecule in the cell and brain.…”

Section: Introductionmentioning

confidence: 99%

“…10 Serum SH-SS and intracellular oxidizedreduced glutathione (GSSG-GSH) balances are markers indicating extracellular and intracellular oxidative stress and antioxidant capacity. 8 Besides removing ROS, glutathione is renewing other antioxidant molecules, and repairing oxidized cell contents. 11 Thus, low glutathione levels are associated with decreased growth, differentiation, and increased apoptosis in neuron cells.…”

Section: Introductionmentioning

confidence: 99%

The relationship between rehabilitation outcomes and extracellular thiol-disulphide and intracellular oxidized-reduced glutathione homeostasis in patients with subacute stroke

Alışık¹,

Alışık²,

Cogalgil³

2023

NeuroAsia

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Background & Objective: To evaluate the relationship between the clinical outcome of subacute stroke patients and extracellular thiol-disulphide (SH-SS) and intracellular oxidized-reduced glutathione (GSSG-GSH) homeostasis and the effect of the rehabilitation program on these homeostasis. Methods: In this prospective observational study, outcome assessments (National Institutes of Health Stroke Scale Scores (NIHSS), modified Rankin Scale (mRS), and Barthel Daily Living Activities Index (BI)) and SH-SS and GSSG-GSH homeostasis parameters were investigated from 42 patients with subacute stroke before and after 4-week rehabilitation treatment protocol. Also, SH-SS and GSSG-GSH homeostasis parameters were measured from 35 healthy volunteers. Results: SS/SH and GSSG/GSH ratios were significantly higher in the patient group at baseline and post-rehabilitation than the control group (p<0.05). SS/SH ratio, GSSG/GSH ratio, NIHSS and mRS values significantly decreased with the rehabilitation (p<0.05). The baseline SS/SH and GSSG/GSH ratios were correlated with baseline and post-rehabilitation NIHSS, mRS and BI scores (p<0.05). Conclusions: The better outcomes of patients with higher baseline antioxidant thiol groups suggest that active thiol groups may play a role in achieving better outcomes in subacute stroke. In addition, rehabilitation treatment increased clinical scores and additionally shifted intracellular and extracellular thiol groups to the antioxidant side.

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Erythrocyte reduced/oxidized glutathione and serum thiol/disulfide homeostasis in patients with rheumatoid arthritis

Cited by 13 publications

References 33 publications

Estrogen receptors, ERK1/2 phosphorylation and reactive oxidizing species in red blood cells from patients with rheumatoid arthritis

Estrogen receptors, ERK1/2 phosphorylation and reactive oxidizing species in red blood cells from patients with rheumatoid arthritis

Turn-on signal fluorescence sensor based on DNA derived bio-dots/polydopamine nanoparticles for the detection of glutathione

The relationship between rehabilitation outcomes and extracellular thiol-disulphide and intracellular oxidized-reduced glutathione homeostasis in patients with subacute stroke

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