Erythrocytic abnormalities in three Antarctic penguin species along the Antarctic Peninsula: biomonitoring of genomic damage

Mas, Eva De; Benzal, Jesús; Merino, Santiago; Valera, Francisco; Palacios, María José; Cuervo, José Javier; Barbosa, Andrés

doi:10.1007/s00300-015-1667-2

Cited by 23 publications

(8 citation statements)

References 46 publications

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“…Our results suggest that during embryonic development individuals are more susceptible to genotoxic effects of Hg. The ENA levels we report were relatively high, for instance higher than the ones reported for penguins exposed to heavy metals in field conditions (De Mas et al 2015). However, micronuclei frequencies were below the threshold levels reviewed in the literature (less than 2.14 micronuclei/1000 erythrocytes) as typical for healthy birds (Zúñiga‐González et al 2000, 2001; Quirós et al 2008).…”

Section: Discussioncontrasting

confidence: 79%

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Mercury Uptake Affects the Development of Larus fuscus Chicks

Santos

Sotillo

Gupta

et al. 2020

Enviro Toxic and Chemistry

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Current emission and mobilization rates of mercury (Hg) in the environment pose extensive threats to both wildlife and human health. Assessing the exposure risk and effects of Hg contamination in model species such as seabirds is essential to understand Hg risks at the population and ecosystem levels. The lesser black-backed gull (Larus fuscus), a generalist seabird species, is an excellent model species because it forages in both marine and terrestrial habitats, which in turn differ in their Hg exposure risk. To identify possible deleterious effects of Hg exposure on developing L. fuscus chicks, a dietary experiment was carried out and chicks were provided a marine, terrestrial, or mixed diet. The effects of embryonic and dietary Hg exposure on chick body condition and physiological state were assessed at different developmental stages until fledging age (30 d). Overall physiological condition was lower in chicks fed a predominantly marine diet, which coincided with higher Hg loads in blood and primary feathers. However, no effect of dietary uptake of Hg was observed on body condition or in terms of genotoxic damage. Body condition and genotoxic damage correlated instead with Hg exposure during embryonic development, which seems to indicate that embryonic exposure to Hg may result in carry-over effects on later chick development.

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Section: Discussioncontrasting

confidence: 79%

“…(Shugart et al 2003; Baos et al 2006). More recently, the frequency of ENAs has been used as a marker to detect genotoxic damage in birds (De Mas et al 2015; Santos et al 2017b). Although its genotoxic effects in birds have been scarcely addressed in the literature, Hg has been suggested to have genotoxic effects (e.g., Fenstad et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Mercury Uptake Affects the Development of Larus fuscus Chicks

Santos

Sotillo

Gupta

et al. 2020

Enviro Toxic and Chemistry

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“…Micronuclei were also analyzed separately because they are the most frequently studied erythrocyte abnormality (see De Mas et al 2015).…”

Section: Micronucleus Assay and Nuclear Abnormalitiesmentioning

confidence: 99%

Chronic Degradation of Seasonally Dry Tropical Forests Increases the Incidence of Genotoxicity in Birds

Solórzano

Ordóñez-Delgado

Espinosa

et al. 2023

Preprint

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Environmental stress as a consequence of anthropic pressures can affect the physiological condition of animals and cause damage to their DNA. Multiple studies have shown genotoxic effects of different pollutants; however, to our knowledge, the impact of environmental stress on genetic damage has been scarce explored. In some stressful ecosystems, such as seasonally dry tropical forests, the combined effects of anthropogenic activities and ongoing global changes cause increased environmental stresses that could trigger physiological and genetic effects on biodiversity. In this study, we evaluated the changes in the prevalence of genotoxic damage to birds in three states of forest degradation in the Tumbesian Region of Western Ecuador. We used the blood samples of 52 bird species to determine the frequency of micronucleus and nuclear abnormalities in the erythrocytes. Our results showed a significant effect of forest degradation in the proportion of micronucleus and nuclear abnormalities at the community level, localities with higher degradation showed higher levels of abnormalities. However, when analyzing the dominant species, we found contrasting responses. While Amazilis amazilia and Lepidocolaptes souleyetii showed a reduction in the proportion of nuclear abnormalities from the natural to shrub-dominated localities, Troglodytes aedon and Polioptila plumbea showed an increase. We concluded that the degradation process of these tropical forests increases the stress on the bird community generating genotoxic damage to the bird community. Bird responses seem species-specific, which could explain the differences in changes in bird composition reported in other studies.

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“…Interestingly, relatively little attention has been paid so far to wild bird populations in this regard. Although several molecular techniques have been successfully employed in avian studies to evaluate molecular alterations induced by various genotoxic contaminants [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ], there is a paucity of work on novel molecular techniques that would allow us to verify the genotoxic properties of lead. Microsatellite instability (MSI) could be potentially effective as such a novel method of detection of genotoxicity of various compounds present in the environment.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite Instability Assay as a Potential Approach to Evaluate Genotoxicity: Lead Exposure in a Nestling Passerine Bird at the Stage of Intensive Erythropoiesis

Markowski

Kaliński

Wawrzyniak

et al. 2023

Animals

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Although many avian studies have investigated the toxic effects of lead on important biochemical and physiological processes, organ and system function, and behavior, studies evaluating the specific genotoxic effects of exposure to lead are scarce. Nowadays, rapid technological advances can provide new molecular techniques in this regard. In this study, as a novel approach in bird studies, we used a panel of ten microsatellite loci to investigate the microsatellite instability (MSI) in response to experimental lead intoxication in a common hole-nesting species, the great tit Parus major. For this purpose, an experiment based on an intentional single supplementation of a lead (II) acetate trihydrate compound was conducted, with the use of two different doses, applied to randomly chosen great tit nestlings from randomly selected broods, being at the stage of intensive erythropoiesis. Although this preliminary study did not find any MSI in the seven microsatellite markers retained for the final comparison, it contributes to the examination of this molecular technique in field conditions as being potentially applicable in ecotoxicological bird studies. We believe that certain issues should be considered in finding an explanation for our result. First, the single doses of lead used in this study may have been too weak to induce genetic instability. Second, the panel of microsatellite markers studied may have been unsusceptible to lead genotoxicity in general. Third, the relatively short time interval (5 days) between the experimental procedure (lead exposure) and the sampling of post-exposure material (blood) for genetic analyses could have limited the effect of lead genotoxicity. Further analyzes are needed to verify these findings and to evaluate the scope of application of the MSI analysis in wild bird population studies.

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Erythrocytic abnormalities in three Antarctic penguin species along the Antarctic Peninsula: biomonitoring of genomic damage

Cited by 23 publications

References 46 publications

Mercury Uptake Affects the Development of Larus fuscus Chicks

Mercury Uptake Affects the Development of Larus fuscus Chicks

Chronic Degradation of Seasonally Dry Tropical Forests Increases the Incidence of Genotoxicity in Birds

Microsatellite Instability Assay as a Potential Approach to Evaluate Genotoxicity: Lead Exposure in a Nestling Passerine Bird at the Stage of Intensive Erythropoiesis

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