Erythrodynamics in fish: recovery of the goldfishCarassius auratusfrom acute anemia

Abstract

Goldfish (Carassius auratus) were rendered anemic through immersion in phenylhydrazine∙HCl, a cohort of [3H]thymidine-labelled erythrocytes was established, and recovery followed over a 234-d period. Red blood cell (RBC), hemoglobin (Hb), and hematocrit (Hct) levels increased in biphasic fashion during recovery, rapid increases to plateau values being followed by more modest increases to levels equalling those observed prior to treatment. During the initial rapid phase of response, increased ventilatory and cardiovascular activities probably compensated for deficits in oxygen-carrying capacity but, by elevating blood O2 tension, may have suppressed erythropoiesis. Continuing slow increases in RBC, Hb, and Hct may point to some as yet unidentified alternative mechanism for stimulating red cell formation. During maturation, mean erythrocytic volume decreased, while mean erythrocytic hemoglobin level increased. Cycles of division of circulating juvenile erythrocytes occurred at roughly 56-d intervals, but did not appear to play a major role in elevating blood O2-carrying capacity. Division and karyorrhexis or cell breakdown were loosely correlated. Under the conditions employed, red cell half-life was approximately 80 d.

Life span of circulating blood cells in ginbuna crucian carp (Carassius auratus langsdorfii)

“…3 H] thymidine labelling (Houston & Murad, 1995). Although these data do not correspond completely with our data a relatively long life span of circulating erythrocytes was estimated in comparison with other peripheral blood cells.…”

“…Avery et al (1992) determined the life span of circulating rainbow trout erythrocytes to be approximately 500 days by calculating bile pigment metabolism. Using [ 3 H]thymidine-labelled erythrocytes Houston and Murad (1995) estimated the half-life of goldfish erythrocytes to be approximately 80 days. However, no reports deal with the life span of peripheral blood leukocytes in fish.…”

The effects of long‐term food deprivation on respiration and haematology of the neotropical fish Hoplias malabaricus

“…2(b)]. Increased f R was also observed for the anaemic goldfish Carassius auratus (L.) (Houston & Murad, 1995). Interestingly, traíras possess one of the lowest metabolic costs of gill ventilation measured in teleosts: 3% of the total Ṽ 2 in normoxia and 13% under severe hypoxia (Rantin et al, 1992).…”

Role of high zinc levels in the stress defense of common carp

“…The peripheral blood of fish under normal conditions usually contains a small proportion of immature red blood cells. In individuals that have previously suffered respiratory stress, such as hypoxia or experimentally induced anemia (via bleeding or phenylhydrazine treatment), the population of immature red cells may temporarily be increased [44,45]. Stress erythropoiesis is induced upon blood loss, hemolysis, or oxygen deprivation; in mice, the glucocorticoid receptor is required for stress erythropoiesis [46].…”