Erythropoiesis and thrombopoiesis in the head-kidney of the sea bass (Dicentrarchus labrax L.): An ultrastructural study.

Esteban, M. Ángeles; Meseguer, José; Ayala, Alfonsa Garcı́a; Agulleiro, B.

doi:10.1679/aohc.52.407

Cited by 60 publications

(54 citation statements)

References 47 publications

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“…According to Lay and Baldwin [20], this observation is due to the insufficient oxygen in the control cage, as small erythrocytes enhance oxygen delivery. Esteban et al [34] stated that the erythrocyte maturation process in sea bass mainly involves a decrease in nuclear and cellular size, condensation of the chromatin and a progressive increase in the amount of haemoglobin. According to Lay and Baldwin [20], the haemoglobin concentration is negatively related to the erythrocyte volume and, therefore, small erythrocytes enhance oxygen delivery.…”

Section: Discussionmentioning

confidence: 99%

Improving aeration for efficient oxygenation in sea bass sea cages. Blood, brain and gill histology

et al. 2016

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An air diffusion based system (Airx) was developed to control the dissolved oxygen levels in aquaculture sea cages. The system was introduced and then tested for 37 days in a sea bass sea cage (aerated cage). A second sea bass sea cage, without the AirX, was used as a control. Oxygen levels were measured in both cages at the start of the trial, before the AirX system was introduced, and during the working period of the AirX system. Fish samples were collected 15 days after the AirX system was introduced and at the end of the experiment. Blood smears were prepared and examined microscopically. Erythrocyte major axis, minor axis and area of fish erythrocytes were measured. Leucocyte differentiation was also examined. In the control cage, the fish had significantly larger red blood cells when compared with the red blood cells of the fish in the aerated cage. Histological examination of the gills and brain revealed no morphological differences or alterations between the two groups of fish. This study demonstrated that an air diffuser system could improve the water quality of fish farmed in sea cages and enhance sea bass physiological performance, especially if DO levels fall below 60% oxygen saturation.

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Section: Discussionmentioning

confidence: 99%

Improving aeration for efficient oxygenation in sea bass sea cages. Blood, brain and gill histology

et al. 2016

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“…In agreement with Scapigliati et al [14], in the present study, we have observed in fish reared with 13 mg/L of oxygen, an increment in B lymphocyte percentages in PBL, head kidney, and spleen. The latter organs/tissue are normally more enriched with B-cells than T-cells [17,24]. In particular, the head kidney and spleen represent the main organs where are placed the lymphopoietic foci of B-cells [16,24].…”

Section: Discussionmentioning

confidence: 99%

“…The latter organs/tissue are normally more enriched with B-cells than T-cells [17,24]. In particular, the head kidney and spleen represent the main organs where are placed the lymphopoietic foci of B-cells [16,24]. Contrarily, in our analysis by flow cytometry and immuno-histochemistry, the B-cells seemed to diminish in thymus (just a trend, not statistically significant) and gut (p < 0.05) in the HO group as compared to the NO group.…”

Section: Discussionmentioning

confidence: 99%

Water Oxygen Content Affects Distribution of T and B Lymphocytes in Lymphoid Tissues of Farmed Sea Bass (Dicentrarchus Labrax)

Romano

Scapigliati

Abelli

2017

Fishes

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Abstract:Intensive aquaculture systems have stimulated proper management in order to limit stressful stocking conditions and environmental impact. This should improve fish wellness and counteract adverse water characteristics such as high concentrations of carbon. The oxygen can be considered a key factor since critical conditions leading to chronic rise of blood catecholamines can depress the number and Oxygen-carrying capacity of erythrocytes. In this research, we evaluated the effects of variation in water oxygen (from 6 ± 1 to 13 ± 1 mg/L) on lymphocytes density in sea bass by using flow cytometry and immunohistochemistry. Gut, gill, and thymus T-cells were significantly enhanced in percentage in hyperoxygenated fish compared with normo-oxygenated group, while Peripheral Blood Leukocytes (PBL), head kidney, and spleen T-cells were not significantly different. Contrarily, in PBL, head kidney and spleen hyper-oxygenation treatment provoked an enhancement of B lymphocyte percentages. The distribution of positive T-cells in hyper-oxygenated fish varied in the organs and followed the relative increments/decrements observed in flow cytometry. This study remarks the sensitivity of sea bass adaptive immunocytes to oxygen concentration, evidenced by the modification of parameters. Sea bass culture under high-flow water recycling and at around 12-13 mg/L oxygen concentrations is therefore suggested to enhance the immune response capacity.

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“…siderado o principal órgão formador de células do sangue de teleósteos (Bielek 1981) sendo composto por células hematopoiéticas em diferentes estágios de maturação ocupando o espaço extravascular (Esteban et al 1989). Este órgão mostrou-se como uma massa celular dividida em uma região medular e uma cortical e, na região de transição com o rim apresentou túbulos contorcidos proximais e distais, o que também foi relatado por Zuasti & Ferrer (1988), Fergunson (1995) e Powell (2000 em peixes teleósteos.…”

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Histologia e ultraestrutura do rim e rim cefálico do pacu

Costa

Lima

et al. 2012

Pesq. Vet. Bras.

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The Pacu, Piaractus mesopotamicus, is a teleostei of the Characidae family, intensively cultivated in Brazil due to its rusticity, fast growth and easy adaptation. Morphological knowledge of the body systems including the lymphoid organs is necessary to improve ϐish production and supply subsidies for the maintenance of stocks. This study aimed to describe morphologically kidney and head kidney of Piaractus mesopotamicus, analyzing the cellular proϐile of each organ with the use of light microscopy and electronic transmission microscopy. The result of the macroscopic analysis showed that the localization of the kidney and head kidney is the same as found in the majority of the teleostei. The kidney presented a form in "H", where the medium region is expanded on the natatorium bladder. The head kidney presented a dilatation in the cranial region of the kidney. In the transmission electronic microscopy we found ultrastructural similarities with other teleostei ϐish. It can be concluded that the lymphoid organs (kidney and head kidney) of Piaractus mesopotamicus are histologically and ultra-structurally similar to the other teleostei. sentou uma forma em "H", onde a região média se expandia sobre a bexiga natatória. O rim cefálico se apresentou como uma dilatação na região cranial do rim, mostrando-se bem visível. Na microscopia eletrônica de transmissão também foram observadas similaridades ultraestruturais com outros teleósteos. Observando nossos resultados concluímos que histologicamente e ultraestruturalmente, os órgãos linfóides rim e rim cefálico de Piaractus mesopotamicus são similares aos de outros teleósteos.

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Erythropoiesis and thrombopoiesis in the head-kidney of the sea bass (Dicentrarchus labrax L.): An ultrastructural study.

Cited by 60 publications

References 47 publications

Improving aeration for efficient oxygenation in sea bass sea cages. Blood, brain and gill histology

Improving aeration for efficient oxygenation in sea bass sea cages. Blood, brain and gill histology

Water Oxygen Content Affects Distribution of T and B Lymphocytes in Lymphoid Tissues of Farmed Sea Bass (Dicentrarchus Labrax)

Histologia e ultraestrutura do rim e rim cefálico do pacu

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