Erythropoietin secretion and physiological effect in mouse after intramuscular plasmid DNA electrotransfer

Multiple systemic expression of human interferon-β in mice can be achieved upon repeated administration of optimized pcTG90-lipoplex

“…Using our optimized lipoplex formulation, levels of human IFN-␤ found in mice serum (20-100 IU/ml or 1-5 ng/ml) were well in the range of therapeutic levels for IFN-␤, 6 and other circulating proteins such as transforming growth factor-␤1, 15 human factor VIII, 16 erythropoietin, 17 or endostatin. 18 Levels of circulating human IFN-␤ were detectable for about 3 days following lipoplex injection.…”

Optimisation of electrotransfer of plasmid into skeletal muscle by pretreatment with hyaluronidase – increased expression with reduced muscle damage

“…Hence, where plasmid gene transfer into skeletal muscle is carried out for induction of an immune response (genetic vaccination), or for systemic delivery (eg apoE, factor IX, erythropoietin), the damage caused when using current electrotransfer protocols may not be significantly detrimental. 11,[13][14][15] However, for treatment of muscle disease by gene replacement, for example, in the case of muscular dystrophies, it is essential to ensure the survival of muscle fibres while maximising the number of transfected fibres.…”

Non-viral gene transfer of murine spleen cells achieved by in vivo electroporation

“…16 Aihira et al 17 and Mir et al 18,19 simultaneously reported efficient plasmid DNA electrotransfer into muscle fibers. To use muscle as a secretory tissue was shown to be efficient also when DNA plasmid was electrotransfered 9,11,[20][21][22] and for vaccination. 12 Lastly, secreted transgenic proteins encoded by the electrotransfered DNA plasmids are able to induce physiological effects.…”