Erythrothrombocytapheresis and Plasmathrombocytapheresis with Storage in T-sol of Platelets Collected by the New Amicus Cell Separator

Abstract

The Amicus cell separator is the latest apparatus introduced into the international market for high-yield, low WBC contamination and short-procedure time thrombocytapheresis. In its original configuration the apparatus collects platelets for subsequent resuspension in plasma and no collection of PRBC can be carried out along with thrombocytapheresis. In this paper we present the results of plasma-thrombocytapheresis and erythro-thrombocytapheresis after the adaptation of the Amicus to the collection and storage of platelets in a non-plasma medium and the concurrent collection of PRBC. From our study it is concluded that PRBC collection doesn't modify the quality of the platelet product obtained from random donors (platelets pre-count 263×10e3/μl) in terms of yield which is 4.6×10e11 platelets, WBC contamination (1.3×10e5) or procedure time (63±26 min.). The quality of the platelet products is satisfactory too, as measured by aggregation induced by collagen and ristocetin or by the substantial stability of membrane glycoproteins (CD62 - 63 - 51 - 36 - 42B). The concentrate had an average content of 58.8 g of hemoglobin per bag, a final volume of 376 ± 13 ml, (after the addition of 100 ml of SAG-M) and a normal mechanic and osmotic fragility.

Hyperconcentrated platelets stored in additive solution: aspects on productivity and in vitro quality

“…The CEs of our DP collections comply well with published CEs for double PLT collections, ranging between 44 and 73% for different apheresis devices [23–31]. In the two studies reporting CEs for DP collections using the Dideco Excel apparatus and Baxter's AMICUS, the CEs were 52 and 69%, respectively [7,8]. Therefore, our results for TRIMA V4 fit well within this range.…”

“…In all storage media, P‐selectin expression on day 0 was similarly high, at 34–37%. Other authors collecting DPs at lower PLT concentrations (2500–4000 × 10 3 PLTs/µl) using TRIMA, the Dideco Excel apparatus, Baxter's AMICUS, or Baxter's CS3000‐Plus, reported lower P‐selectin expression rates on day 0, ranging between 4·5 and 7·8%[6–9]. However, as the type of measurement of P‐selectin expression can be very different, comparison between the results of different studies may be of limited value.…”

“…To date, little data exists on the production of DPs using widely used apheresis devices [3–9]. Additionally, there is still a lack of data on the storage of apheresis‐derived DPs in PASs.…”

Harvesting of CD34 antigen‐expressing cells with a new programme for the collection of mononuclear cells with use of the Amicus (Baxter) blood cell separator

“…The Amicus cell separator was built to replace the CS 3000 device, which has been used over years for PBSC harvesting (Lasky et al ., 1989; Rosenfeld et al ., 1994; Lin et al ., 1995). First, the PLT collection was developed for the Amicus machine, and it showed a high PLT yield and collection efficiency (Burgstaler et al ., 1997; Valbonesi et al ., 1997; Yockey et al ., 1998; Moog & Müller, 1999). Now, a programme for mononuclear cell harvesting was developed, and the aim of the present study was to evaluate this programme for the collection of autologous and allogeneic PBSCs.…”

Erythroplateletapheresis with the Amicus cell separator

“…This last limitation is of particular relevance with the Amicus that, for its high collection efficiency, may be routinely employed for double plateletapheresis. We have adapted the Amicus for resuspension of the PC in a nonplasma medium, T-Sol, and for collection of red blood cells (RBC) [2]. Seventy-five donors (Group A) (35 men and 40 women) were enrolled for plasmaplateletapheresis, and 45 donors (Group B) (30 men and 15 women) for erythrothrombocytapheresis.…”