Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı ve Cryptosporidium Türlerinin Moleküler Karekterizasyonu

Güven, Esin; Avcıoğlu, Hamza; Balkaya, İbrahim; Hayırlı, A.; Kar, Sırrı; Karaer, Zafer

doi:10.9775/kvfd.2013.9187

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…Today, in parallel with the modern developments in statistical calculations, some progress has been made in the Bootstrap method and its use in applied statistics has become widespread. The use of the Bootstrap method has increased highly especially in the areas of molecular genetics [7][8][9] , population genetics [10] , phylogenetics [11] and virology [12] .…”

Section: Introductionmentioning

confidence: 99%

Hayvancılık Alanında Bootstrap Tekniğinin Bir Uygulaması: Yumurta Sarı Rengi Örneği

Narinç¹,

Aygün²,

Küçükönder³

et al. 2015

Kafkas Univ Vet Fak Derg

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In this study, it was aimed to introduce the Bootstrap technique and to reveal the relationship between measurements of yolk color fan grades and digital colorimeter that is used for determining the yellow color of egg by utilizing this technique. For this purpose, a total of 1350 samples of 15 color grades of Roche yolk color fan and L* (lightness), a* (redness), b* (yellowness) values in the same samples were compared. The means, standard errors and confidence intervals for each color parameters of fan grades have been demonstrated by the Bootstrap technique. The grades of Roche yolk color fan in terms of L* values have been divided into 10 groups (P<0.01), while only divided into 9 groups in terms of b* values (P<0.01). According to the means of Redness (a*), all of the Roche yolk color fan grades (15 grades) have been determined as independent from each other (P<0.01). With the Bootstrap method, the standard error values of means were decreased by 42.03%, 35.38% and 30.24%, respectively, and the confidence intervals were narrowed by the ratio of 42.03%, 35.38% and 30.24%, respectively. The results of the study were compared with the results of the study that was conducted by using Roche yolk color fan which is cheaper but less reliable and by using digital colorimeter method which is expensive but reliable. Keywords: Bootstrapping, Resampling, Egg yolk color fan, Digital colorimeter Hayvancılık Alanında Bootstrap Tekniğinin Bir Uygulaması: Yumurta Sarı Rengi Örneği ÖzetBu çalışmada Bootstrap tekniğinin tanıtılması ve bu metottan faydalanarak yumurta sarı renginin belirlenmesinde kullanılan yumurta sarı renk yelpazesi ve dijital renk ölçüm cihazı ölçümleri arasındaki ilişkinin ortaya konulması amaçlanmıştır. Bu amaçla Roche sarı renk yelpazesinin 15 renk sınıfına ait toplam 1350 örnek ile aynı örneklerde ölçülen L* (parlaklık), a* (kırmızılık), b* (sarılık) değerleri karşılaştırılmıştır. Yelpaze sınıflarının her renk parametresine ait ortalamaları, standart hataları ve güven aralıkları Bootstrap tekniğiyle ortaya konulmuştur. L* değerleri bakımından Roche sarı renk yelpazesi 10 gruba ayrılırken (P<0.01), b* değerleri bakımından sınıflar sadece 9 gruba ayrılmıştır (P<0.01). Kırmızılık (a*) ortalamalarına göre ise Roche sarı renk yelpaze sınıflarının tümü (15 sınıf ) birbirinden bağımsız olarak tanımlanabilmiştir (P<0.01). Bootstrap yöntemi ile L*, a* ve b* ortalamalarının standart hata değerleri sırasıyla 42.03%, 35.38% ve 30.24% azalmış, güven aralıkları 48.10%, 55.43%, 34.47% oranlarında daralmıştır. Araştırmadan elde edilen sonuçlar kullanılarak mevcut literatürde daha ucuz ama güvenilirliği düşük olan Roche sarı renk yelpazesi ile yapılan çalışmalar ve pahalı ama güvenilir yöntem olan dijital renk ölçüm cihazı kullanılarak gerçekleştirilen çalışma sonuçları karşılaştırılmıştır.

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Section: Introductionmentioning

confidence: 99%

Hayvancılık Alanında Bootstrap Tekniğinin Bir Uygulaması: Yumurta Sarı Rengi Örneği

Narinç¹,

Aygün²,

Küçükönder³

et al. 2015

Kafkas Univ Vet Fak Derg

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Cryptosporidium spp. in Dogs - Prevalence and Genotype Distribution

Çelik,

Akın Koçhan,

Burçak Aslan Çelik

et al. 2023

Acta Scientiae Vet.

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Background: Cryptosporidium spp. is a zoonotic protozoan parasite that affects the gastrointestinal tract of humans and animals. The disease can cause acute and chronic diarrhoea and even death in both humans and animals. In this study, it was aimed to determine the prevalence and genotype distribution of Cryptosporidiosis in shelter dogs in Diyarbakır province located in the Southeastern Anatolia Region of Turkey. Materials, Methods & Results: The animal material of the study consisted of 100 dogs of different breeds and sexes. Faecal samples were collected from the rectum with disposable latex gloves and placed in individual sample containers. All of the samples were examined for Cryptosporidium spp. by Kinyoun Acid Fast and Nested PCR methods. In the Kinyoun Acid Fast staining method, firstly, smear preparations were prepared from fresh faecal samples, fixed in pure methanol for 1 min and allowed to dry. The slides were kept in Kinyoun Carbol-Fuxin for 5 min, dipped in 50% ethyl alcohol, shaken, washed in tap water, kept in 1% sulphuric acid for 2 min and washed in tap water. The slides were kept in methylene blue for 1 min, washed in tap water and allowed to dry. After drying, immersion oil was dripped and examined under a microscope at 100 magnification. DNA extraction was performed from all samples using GeneMATRIX Stool DNA Purification Kit according to the manufacturer's protocol. After Nested PCR analysis was performed. In the PCR step, primers 5'-TTCTAGAGCTAATACATGCG-3' and 5'- CCCATTTCCTTCCTTCGAAACAGGA-3' were used to amplify the 1325 bp gene region. In the nested PCR step, primers 5'- GGAAGGGTTGTATTTATTTATTAGATAAAG-3' and 5'-AAGGAGTAAGGAACAACCTCCA-3' were used to amplify the 826-864 bp gene region. As a result of both methods, a prevalence of 3% was determined. The infection rate was higher in males (3.57%) than females (2.27%) and in younger than 1 year (5.56%) than in older than 1 year (1.56%). The DNA sequences obtained from the sequence analysis of 3 positive PCR samples were analysed in BioEdit software. A phylogenetic tree was constructed with the data set created by using the 18s rRNA gene sequences obtained from the NCBI genbank database and the DNA sequences obtained as a result of the study, and it was shown which Cryptosporidium species the study samples were related to. Today, many Cryptosporidium species have been identified and most of these species have host adaptation. Although C. canis is the most common species in dogs, C. muris, C. meleagridis, and C. parvum have also been detected. Among these species, C. parvum is recognized as a zoonotic species infecting a wide range of mammals. In this study, DNA sequencing of nested PCR positive samples revealed that 3 samples were zoonotic C. parvum. Discussion: This suggests that dogs may be a reservoir for zoonotic transmission of Cryptosporidium. Consequently, it is recommended that people should be informed about the potential for transmission of this protozoan to humans and animals and that control programmes should be implemented, including the prevention of free entry of stray dogs into public places and homes. Keywords: Cryptosporidium parvum, molecular analysis, canine, Diyarbakır, Turkey.

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ELISA–based Point Prevalence of enteropathogens in diarrheic calves in Central Anatolia Region of Turkey

Işik–Uslu,

Derinbay–Ekici,

Avci

2023

RC FCV-LUZ

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This study reports potential causes of diarrhea in neonatal calves, leading to calf mortality, from the selected population of the three Provinces of Turkey. A total of 300 fecal samples were collected purposively from diarrheic neonatal calves distributed to the three age groups (1–14 days, 15–29 days, and 30–90 days), from Konya, Karaman, and Aksaray Provinces of Turkey. The fecal specimens were examined for the existence of Cryptosporidium spp., rotavirus, coronavirus, and Escherichia coli by commercially available capture direct enzyme linked immunosorbent assay (ELISA) kit. The oocysts and coproantigens of Cryptosporidium were identified in 109 (36.3%) and 156 (52%) of the 300 calves, respectively. While, rotavirus, E. coli and coronavirus antigens were detected (P<0.05) in 57 (19%), 17 (5.6%) and 6 (2%) calves, respectively. Mixed infection of the study pathogens has also been found in this report. These results provide a baseline information on the frequent causes of neonatal calf diarrhea in the studied Provinces which can be used to develop a prophylaxis plan.

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Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı ve Cryptosporidium Türlerinin Moleküler Karekterizasyonu

Cited by 4 publications

References 25 publications

Hayvancılık Alanında Bootstrap Tekniğinin Bir Uygulaması: Yumurta Sarı Rengi Örneği

Hayvancılık Alanında Bootstrap Tekniğinin Bir Uygulaması: Yumurta Sarı Rengi Örneği

Cryptosporidium spp. in Dogs - Prevalence and Genotype Distribution

ELISA–based Point Prevalence of enteropathogens in diarrheic calves in Central Anatolia Region of Turkey

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